Carcinoma‐in‐situ cells in cultured human seminiferous tubules

Abstract

For the first time, early germ-cell derived tumour cells were studied in an in-vitro system of cultured seminiferous tubules. The intratubular tumour cells not only survived in culture for 7 days but were also able to multiply. Dividing tumour cells were identified in semi-thin sections and electron micrographs by morphological criteria. Additionally, mitotic activity was demonstrated by [3H]thymidine histo-autoradiography. There are numerous reports on cell lines established from solid non-seminomas, but up to now no references to seminoma cell lines or cultures of intratubular tumour cells are available. The culture of seminiferous tubules offers a tool in making carcinoma-in-situ cells accessible for experimental work.

Prevalence of Contralateral Testicular Intraepithelial Neoplasia (Carcinoma in situ) in Patients with Testicular Germ Cell Tumour

“…There are only weak clinical markers such as age below 30 years, history of bilateral cryptorchidism [3], and increased serum follicle-stimulating hormone (FSH) [4] that may identify patients at a particular high risk for a sec ond testicular cancer. After Skakkebaek's first description of carcinoma in situ of the testis (CIS) in 1972 [5], the concept of CIS being the uniform precursor of testicular GCT [6] has gained increas ing acceptance [1,[7][8][9][10][11][12], CIS has been shown to be present in a testis many years before the clinical manifestation of a tumour. It has been demonstrated that once a testicle is afflicted with CIS the majority of the seminiferous tubules are likely to contain these cells [12], Thus, a random surgi Terminology Skakkebaek, in 1972, used the term 'carcinoma in situ' when he first described atypical large spermatogonia [5] that were later shown to be precursor cells of testicular patients with condition based on total number of patients examined.…”

Hanging drop cultures of human testis and testis cancer samples: a model used to investigate activin treatment effects in a preserved niche

“…Several groups have previously established human testis tissue cultures (Steinberger, 1975; Kierszenbaum, 1994; Staub, 2001; Sofikitis et al , 2005), as well as CIS and seminoma tissue cultures (Lauke et al , 1991; Olie et al , 1995, 1996; Rajpert-De Meyts et al , 1998). However, the majority of these approaches had limited success and have not been systematically used.…”

Testicular Intraepithelial Neoplasia: The Precursor of Testicular Germ Cell Tumors

“…In most of the cases, TIN cells spread in a noninvasive manner longitudinally inside the seminiferous tu bules until they reach the rete testis and usually all spermiogenic cells are replaced in the tubules afflicted, leaving behind only TIN cells and Sertoli cells. After reaching the rete testis, it is likely that the TIN cells gain access to neighboring semini ferous tubules, though this assumption has been challenged [34,35], There is some evidence that repair mechanisms like macrophage activity eliminate a number of the TIN cells [35] and. possibly, in some cases, all TIN cells are eliminated.…”