CARD9-Associated Dectin-1 and Dectin-2 Are Required for Protective Immunity of a Multivalent Vaccine against <i>Coccidioides posadasii</i> Infection

Campuzano, Althea; Zhang, Hao; Ostroff, Gary R.; Dias, Lucas Dos Santos; Wüthrich, Marcel; Klein, Bruce S.; Yu, Jieh Juen; Lara, Humberto H; Lopez-Ribot, José L.; Hung, Chiung Yu

doi:10.4049/jimmunol.1900793

Cited by 27 publications

(49 citation statements)

References 66 publications

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“…To bridge this gap, transgenic HLA mice were applied as reliable animal models in pre-clinical investigation of vaccines (44). For example, a vaccine against Coccidioides posadasii was generated using human Th17-driven epitopes (45), and its protective effect was evaluated in humanized HLA-DR4 transgenic mice (46). Adequate investments are needed to evaluate the efficacy of PVAC in HLA class I and class II humanized transgenic mice in order to develop a human vaccine against PA in the future.…”

Section: Discussionmentioning

confidence: 99%

Development of a Chimeric Vaccine Against Pseudomonas aeruginosa Based on the Th17-Stimulating Epitopes of PcrV and AmpC

et al. 2021

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Pulmonary infection caused by Pseudomonas aeruginosa (PA) has created an urgent need for an efficient vaccine, but the protection induced by current candidates is limited, partially because of the high variability of the PA genome. Antigens targeting pulmonary Th17 responses are able to provide antibody-independent and broad-spectrum protection; however, little information about Th17-stimulating antigens in PA is available. Herein, we identified two novel PA antigens that effectively induce Th17-dependent protection, namely, PcrV (PA1706) and AmpC (PA4110). Compared to intramuscular immunization, intranasal immunization enhanced the protection of rePcrV due to activation of a Th17 response. The Th17-stimulating epitopes of PcrV and AmpC were identified, and the recombinant protein PVAC was designed and generated by combining these Th17-stimulating epitopes. PVAC was successfully produced in soluble form and elicited broad protective immunity against PA. Our results provide an alternative strategy for the development of Th17-based vaccines against PA and other pathogens.

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Section: Discussionmentioning

confidence: 99%

Development of a Chimeric Vaccine Against Pseudomonas aeruginosa Based on the Th17-Stimulating Epitopes of PcrV and AmpC

et al. 2021

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“…Arthroconidia are the infectious propagules of Coccidioides. Infection of murine models through intranasal delivery is the preferred and widely used method to understand Coccidioides pathology (Campuzano et al, 2020;Castro-Lopez & Hung, 2017;Hayden et al, 2019;Mead, Roe, et al, 2020;Viriyakosol, Kapoor, et al, 2019). Susceptibility to Coccidioides varies among mouse strains, with studies having shown that C57BL/6 and BALB/c mice are highly susceptible and that DBA/2 mice are more resistant (Kirkland & Fierer, 1983).…”

Section: Intranasal Infection Of Murine Modelsmentioning

confidence: 99%

Proper Care and Feeding of Coccidioides: A Laboratorian's Guide to Cultivating the Dimorphic Stages of C. immitis and C. posadasii

2020

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Coccidioides immitis and C. posadasii. These fungi are thermally dimorphic, cycling between mycelia and arthroconidia in the environment and converting into spherules and endospores within a host. Coccidioides can cause a broad spectrum of disease that can be difficult to treat. There has been a steady increase in disease, with an estimated 350,000 new infections per year in the United States. With the increase in disease and difficulty in treatment, there is an unmet need to increase research in basic biology and identify new treatments, diagnostics, and vaccine candidates. Here, we describe protocols required in any Coccidioides laboratory, such as growing, harvesting, and storing the different stages of this dimorphic fungal pathogen.

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“…This is then incorporated into glucan–chitin particles derived from Rhodotorula mucilaginous , which acts as the adjuvant. Preliminary studies in mice have shown protection against strains of both C. immitis and C. posadasii [ 90 ], stimulation of the Th1/Th17 pathway [ 91 ], and activation of the CARD9 dectin-1 and dectin-2 signaling pathways [ 92 ]. Finally, no significant toxicity has been seen in a human liver cell line (HepG2) (personal communication, C-Y Hung).…”

Section: Vaccinesmentioning

confidence: 99%

Coccidioidomycosis: Changing Concepts and Knowledge Gaps

Ampel

2020

JoF

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Although first described more than 120 years ago, much remains unknown about coccidioidomycosis. In this review, new information that has led to changing concepts will be reviewed and remaining gaps in our knowledge will be discussed. In particular, new ideas regarding ecology and epidemiology, problems and promises of diagnosis, controversies over management, and the possibility of a vaccine will be covered.

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CARD9-Associated Dectin-1 and Dectin-2 Are Required for Protective Immunity of a Multivalent Vaccine against Coccidioides posadasii Infection

Abstract: This information is current as Infection posadasii Coccidioides Multivalent Vaccine against Are Required for Protective Immunity of a CARD9-Associated Dectin-1 and Dectin-2 Hung

Cited by 27 publications

References 66 publications

Development of a Chimeric Vaccine Against Pseudomonas aeruginosa Based on the Th17-Stimulating Epitopes of PcrV and AmpC

Development of a Chimeric Vaccine Against Pseudomonas aeruginosa Based on the Th17-Stimulating Epitopes of PcrV and AmpC

Proper Care and Feeding of Coccidioides: A Laboratorian's Guide to Cultivating the Dimorphic Stages of C. immitis and C. posadasii

Coccidioidomycosis: Changing Concepts and Knowledge Gaps

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