Cardamonin targets KEAP1/NRF2 signaling for protection against atherosclerosis

Abstract

Atherosclerosis (AS)-induced cardiovascular disease is the leading cause of death worldwide. To date, there is still a lack of effective approaches for AS intervention. Cardamonin (CAD) is a bioactive food...

Eicosapentaenoic acid activates the P62/KEAP1/NRF2 pathway for the prevention of diabetes-associated cognitive dysfunction

“…To determine hippocampal protein levels, a western blot was performed as described in our previous study, 23 using primary and secondary antibodies as shown in Table S2 †. The images were analyzed using Image Studio Lite software (LI-COR Biosciences, Lincoln, Nebraska, USA).…”

“…qRT-PCR was performed using RNA extracted from the brain tissue and BV2 cells, as described in our previous work. 23 The primer sequences are provided in Table S3 †…”

“…Molecular docking assays were conducted to evaluate the binding potential and affinity between EPA and the protein P62, following methodologies outlined in our previous study. 23 The structures of EPA and P62 were obtained from the PubChem database (). The potential binding site on P62 was identified based on the existing literature and data.…”