Cardenolide Glycosides from Pergularia tomentosa and Their Proapoptotic Activity in Kaposi's Sarcoma Cells

Abstract

Continuing our investigations on plants belonging to the Asclepiadaceae family, three new cardenolide glycosides, 3'-O-beta-D-glucopyranosylcalactin (1), 12-dehydroxyghalakinoside (2), and 6'-dehydroxyghalakinoside (3), along with the known ghalakinoside (4) and calactin (5), were isolated from the roots of Pergularia tomentosa. The structures of these compounds were elucidated by extensive spectroscopic methods including 1D- and 2D-NMR experiments as well as ESIMS analysis. The isolated cardenolides caused apoptotic cell death of Kaposi's sarcoma cells.

Cardenolides from Gomphocarpus sinaicus and Pergularia tomentosa (Apocynaceae: Asclepiadoideae) deter the feeding of Spodoptera littoralis

“…Three of the cardenolides (1-3) isolated during this study have previously been found in G. sinaicus (El-Askary et al 1993, 1995aAbdel-Azim et al 1996;Abdel-Azim 1998) and two (4 and 6) in P. tomentosa (Gohar et al 2000;Hamed et al 2006). This is the first report of 16a-acetoxycalotropin (5) from P. tomentosa.…”

“…Cardenolides are commonly found in the Apocynaceae family (Chen et al 1987;Begum et al 1999) and its subfamily, the Asclepiadoideae (Rodriguez- Hahn and Fonseca 1991;El-Askary et al 1993, 1995aAbdel-Azim et al 1996;Hamed et al 2006). Insects feeding on cardenolidecontaining plants are either behaviourally (Helmus and Dussourd 2004) or physiologically adapted (Vaughan and Jungreis 1977;Moore and Scudder 1985;Scudder et al 1986) to deal with cardenolides and often accumulate them in their tissues as an acquired chemical defence against vertebrate predators (for example, Rothschild 1972;Martel and Malcolm 2004).…”

Antiproliferative activity of cardenolide glycosides from Asclepias subulata

“…The compound 1 was identified as 12, 16-dihydroxycalotropin and the present study constitute the first report of this cardenolide glycoside. The identities of known compounds calotropin (2), corotoxigenin 3-O-glucopyranoside (3) and desglucouzarin (4) were established by comparison of their MS, 1 H NMR, 13 C NMR, HSQC and HMBC spectroscopic data with published data (Elgamal et al, 1999;Hamed et al, 2006;Karkare et al, 2007).…”

Enzymatic and non-enzymatic antioxidant activity of Pergularia tomentosa against carbon tetra chloride induced hepatic damage in Wistar albino rats

“…The use of natural antioxidants for treating diseases and as food additives has better consumer acceptability and a trend over the use of Pergularia tomentosa L. is an evergreen perennial shrub belong to the family: Asclepiadecae and the height of this shrubby plant species is about one meter with peculiar odour (7). This plant is used in folk remedies as an antirheumatic, laxative, abortive and treatment of some skin diseases, asthma and bronchitis (8). The plant has different biological effects such as cytotoxic, antioxidant and antibacterial activities which are due to its cardenolide and flavonoid compounds (9 -11).…”