Cardenolides content in wild Sardinian<b><i>Digitalis purpurea</i></b>L. populations

Usaï, Marianna; Atzei, A.; Marchetti, Mauro

doi:10.1080/14786410701218291

Cited by 13 publications

(6 citation statements)

References 13 publications

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“…However the digitoxigenin (730 ± 180 mg 100 g -1 dw) and digoxigenin derivatives of cardenolides (490 ± 170 mg 100 g -1 dw) had prevalence in the natural population of D. ferruginea. This was also observed in 2 years old plant of D. purpurea (Usai et al 2007) having ranges of 1.13-24.0 and 0.4-17.8 mg 100 g -1 dw for digitoxigenin and gitoxigenin, respectively. According to these data, it was evident that the predominant cardenolides in D. ferruginea plants were those of the series A, which was in an agreement with previous results reported for other Digitalis species including D. obscura (Gavidia and Pérez-Bermúdez 1997).…”

Section: Natural Populationssupporting

confidence: 54%

Embryogenesis, plant regeneration and cardiac glycoside determination in Digitalis ferruginea subsp. ferruginea L

Verma

Yücesan

Şahin

et al. 2014

Plant Cell Tiss Organ Cult

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The present study reports, for the first time, an efficient in vitro plant regeneration protocol for Digitalis ferruginea subsp. ferruginea L. (rusty foxglove). We have used different concentrations of gibberellic acid (GA 3 ) on Murashige and Skoog (MS) medium to assess the germination frequency of seeds. High frequency of germination was achieved on MS medium with 1.0 mg l -1 GA 3 . 6-Benzylaminopurine (BAP) combined with a-naphtaleneacetic acid (NAA) or 2, 4-dichlorophenoxy acetic acid (2, 4-D) in the induction MS medium induced both somatic embryogensis and shoot organogenesis. The highest percentage of callus growth (85 %) was obtained when hypocotyl explants were cultured on MS medium containing 0.5 mg l -1 2, 4-D plus 1.0 mg l -1 BAP. The maximum mean number of somatic embryos (7.3 ± 1.3 embryos) or shoots (12.0 ± 1.1 shoots) per callus was obtained when medium contained 0.25 mg l -1 NAA plus 1.0 mg l -1 BAP or 0.5 mg l -1 NAA plus 2.0 mg l -1 BAP. The regenerated shoots easily rooted on MS medium. Higher amounts of lanatoside C [13.2 ± 0.5 mg 100 g -1 dry weight (dw)] and digoxin (2.93 ± 0.31 mg 100 g -1 dw) accumulation were obtained when shoots were obtained by indirect regeneration. We also investigated derivatives of cardenolides, i.e., digitoxigenin (730 ± 180 mg 100 g -1 dw), gitoxigenin (50 ± 20 mg 100 g -1 dw) and digoxigenin (490 ± 170 mg 100 g -1 dw) from natural samples.

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Section: Natural Populationssupporting

confidence: 54%

Embryogenesis, plant regeneration and cardiac glycoside determination in Digitalis ferruginea subsp. ferruginea L

Verma

Yücesan

Şahin

et al. 2014

Plant Cell Tiss Organ Cult

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“…It is worthy to note that the unigene encoding CGH was listed among the 25 most abundant unigenes (see Additional file 2 ). Low representativeness of unigenes for cardenolide biosynthesis-associated gene families in our 454 EST database and high expression of the gene involved in primary cardiac glycoside hydrolysis were consistent with overall low cardiac glycoside content in D. purpurea leaves, although relative to other organs leaves have the highest content of cardiac glycosides [ 62 ]. These data greatly enhanced our knowledge of Digitalis cardiac glycoside biosynthesis and provided candidate genes for improving the production of active medicinal compounds in D. purpurea through genetic engineering.…”

Section: Resultsmentioning

confidence: 61%

Comprehensive transcriptome analysis reveals novel genes involved in cardiac glycoside biosynthesis and mlncRNAs associated with secondary metabolism and stress response in Digitalis purpurea

Yan

et al. 2012

BMC Genomics

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BackgroundDigitalis purpurea is an important ornamental and medicinal plant. There is considerable interest in exploring its transcriptome.ResultsThrough high-throughput 454 sequencing and subsequent assembly, we obtained 23532 genes, of which 15626 encode conserved proteins. We determined 140 unigenes to be candidates involved in cardiac glycoside biosynthesis. It could be grouped into 30 families, of which 29 were identified for the first time in D. purpurea. We identified 2660 mRNA-like npcRNA (mlncRNA) candidates, an emerging class of regulators, using a computational mlncRNA identification pipeline and 13 microRNA-producing unigenes based on sequence conservation and hairpin structure-forming capability. Twenty five protein-coding unigenes were predicted to be targets of these microRNAs. Among the mlncRNA candidates, only 320 could be grouped into 140 families with at least two members in a family. The majority of D. purpurea mlncRNAs were species-specific and many of them showed tissue-specific expression and responded to cold and dehydration stresses. We identified 417 protein-coding genes with regions significantly homologous or complementary to 375 mlncRNAs. It includes five genes involved in secondary metabolism. A positive correlation was found in gene expression between protein-coding genes and the homologous mlncRNAs in response to cold and dehydration stresses, while the correlation was negative when protein-coding genes and mlncRNAs were complementary to each other.ConclusionsThrough comprehensive transcriptome analysis, we not only identified 29 novel gene families potentially involved in the biosynthesis of cardiac glycosides but also characterized a large number of mlncRNAs. Our results suggest the importance of mlncRNAs in secondary metabolism and stress response in D. purpurea.

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“…The observation that cardenolide content can differ dramatically in D. purpurea depending on the location of the plant analyzed [101] was confirmed in a more recent study with Sardinian accessions. HPLC analyses of 2-year-old plants revealed that the amounts of digitoxigenin and gitoxigenin in fresh leaves ranged between 11 and 241 mg • kg −1 and 4 and 178 mg • kg −1 , respectively [102].…”

Section: Cardiac Glycosidesmentioning

confidence: 99%

The Foxgloves (Digitalis) Revisited

Kreis

2017

Planta Med

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This review provides a renewed look at the genus Digitalis. Emphasis will be put on those issues that attracted the most attention or even went through paradigmatic changes since the turn of the millennium. PubMed and Google Scholar were used (“Digitalis” and “Foxglove” were the key words) to identify research from 2000 till 2017 containing data relevant enough to be presented here. Intriguing new results emerged from studies related to the phylogeny and taxonomy of the genus as well as to the biosynthesis and potential medicinal uses of the key active compounds, the cardiac glycosides. Several Eastern and Western Foxgloves were studied with respect to their propagation in vitro. In this context, molecular biology tools were applied and phytochemical analyses were conducted. Structure elucidation and analytical methods, which have experienced less exciting progress, will not be considered here in great detail.

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Cardenolides content in wild SardinianDigitalis purpureaL. populations

Cited by 13 publications

References 13 publications

Embryogenesis, plant regeneration and cardiac glycoside determination in Digitalis ferruginea subsp. ferruginea L

Embryogenesis, plant regeneration and cardiac glycoside determination in Digitalis ferruginea subsp. ferruginea L

Comprehensive transcriptome analysis reveals novel genes involved in cardiac glycoside biosynthesis and mlncRNAs associated with secondary metabolism and stress response in Digitalis purpurea

The Foxgloves (Digitalis) Revisited

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