Cardiac 18F-FDG Positron Emission Tomography: An Accurate Tool to Monitor In vivo Metabolic and Functional Alterations in Murine Myocardial Infarction

Fischer, Maximilian; Zacherl, Mathias J.; Weckbach, Ludwig T.; Paintmayer, Lisa; Weinberger, Tobias; Stark, Konstantin; Maßberg, Steffen; Bartenstein, Peter; Lehner, S.; Schulz, Christian; Todica, Andrei

doi:10.3389/fcvm.2021.656742

Cited by 6 publications

(3 citation statements)

References 46 publications

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“…One important and common consideration when using MI animal models to study heart failure is the ability to determine infarct size in vivo using noninvasive methods. Although cardiac magnetic resonance imaging (MRI) and positron emission tomography (PET) have been developed for small rodents (Bohl et al., 2009 ; Fischer et al., 2021 ), these methods are expensive and difficult to employ for longitudinal assessment studies. Recent advances in 4DUS echocardiography, however, have made noninvasive longitudinal assessment of infarct size in mice both feasible and reliable, even with complex morphological changes (Dann et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

4D ultrasound‐based strain assessment of cardiac dysfunction in male rats with reperfused and nonreperfused myocardial infarction

Omoto,

Earl,

Richards

et al. 2024

Physiological Reports

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Two‐dimensional ultrasound (2DUS) echocardiography is the main noninvasive method used to evaluate cardiac function in animal models of myocardial infarction (MI). However, 2DUS echocardiography does not capture regional differences in cardiac contractility since it relies on planar images to estimate left ventricular (LV) geometry and global function. Thus, the current study was designed to evaluate the efficacy of a newly developed 4‐dimensional ultrasound (4DUS) method in detecting cardiac functional differences between two models of MI, permanent ligation (PL), and ischemia/reperfusion (I/R) in rats. We found that only 4DUS was able to detect LV global functional differences between the two models and that 4DUS‐derived surface area strain accurately detected infarcted regions within the myocardium that correlated well with histological infarct size analysis. We also found that 4DUS‐derived strain, which includes circumferential, longitudinal, and surface area strain, correlated with the peak positive of the first derivative of left ventricular pressure (+dP/dtmax). In conclusion, 4DUS strain echocardiography effectively assesses myocardial mechanics following experimentally induced ischemia in rats and accurately estimates infarct size as early as 1 day after injury. 4DUS also correlates well with +dP/dtmax, a widely used marker of cardiac contractility.

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Section: Discussionmentioning

confidence: 99%

4D ultrasound‐based strain assessment of cardiac dysfunction in male rats with reperfused and nonreperfused myocardial infarction

Omoto,

Earl,

Richards

et al. 2024

Physiological Reports

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show abstract

“…The software ‘Inveon acquisition and research workplace’ was used for technical processing. Evaluation of the reconstructed images was performed using the commercially available software QPS/QGS (QUAD, Los Angeles, CA, USA), as described previously [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Modulation of Rxrα Expression in Mononuclear Phagocytes Impacts on Cardiac Remodeling after Ischemia-Reperfusion Injury

et al. 2022

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Retinoid X receptors (RXRs), as members of the steroid/thyroid hormone superfamily of nuclear receptors, are crucial regulators of immune response during health and disease. RXR subtype expression is dependent on tissue and cell type, RXRα being the relevant isoform in monocytes and macrophages. Previous studies have assessed different functions of RXRs and positive implications of RXR agonists on outcomes after ischemic injuries have been described. However, the impact of a reduced Rxrα expression in mononuclear phagocytes on cardiac remodeling after myocardial infarction (MI) has not been investigated to date. Here, we use a temporally controlled deletion of Rxrα in monocytes and macrophages to determine its role in ischemia-reperfusion injury. We show that reduced expression of Rxrα in mononuclear phagocytes leads to a decreased phagocytic activity and an accumulation of apoptotic cells in the myocardium, reduces angiogenesis and cardiac macrophage proliferation in the infarct border zone/infarct area, and has an impact on monocyte/macrophage subset composition. These changes are associated with a greater myocardial defect 30 days after ischemia/reperfusion injury. Overall, the reduction of Rxrα levels in monocytes and macrophages negatively impacts cardiac remodeling after myocardial infarction. Thus, RXRα might represent a therapeutic target to regulate the immune response after MI in order to improve cardiac remodeling.

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“…Researchers examined a decline in left ventricular metabolic volume in the first days after MI. This phenomenon is possible because of remodelling and hypertrophic reaction to maintain LV stroke volume [117]. Despite the decrease in volume, there is a significant increase in glucose demand in the incident zone, with critical changes during the first fourteen days.…”

Section: Cardiac Metabolism Imaging Using [ 18 F]-based Tracersmentioning

confidence: 99%

Recent Advances in Cardiovascular Diseases Research Using Animal Models and PET Radioisotope Tracers

Wargocka-Matuszewska

Uhrynowski

Rozwadowska

et al. 2022

IJMS

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Cardiovascular diseases (CVD) is a collective term describing a range of conditions that affect the heart and blood vessels. Due to the varied nature of the disorders, distinguishing between their causes and monitoring their progress is crucial for finding an effective treatment. Molecular imaging enables non-invasive visualisation and quantification of biological pathways, even at the molecular and subcellular levels, what is essential for understanding the causes and development of CVD. Positron emission tomography imaging is so far recognized as the best method for in vivo studies of the CVD related phenomena. The imaging is based on the use of radioisotope-labelled markers, which have been successfully used in both pre-clinical research and clinical studies. Current research on CVD with the use of such radioconjugates constantly increases our knowledge and understanding of the causes, and brings us closer to effective monitoring and treatment. This review outlines recent advances in the use of the so-far available radioisotope markers in the research on cardiovascular diseases in rodent models, points out the problems and provides a perspective for future applications of PET imaging in CVD studies.

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Cardiac 18F-FDG Positron Emission Tomography: An Accurate Tool to Monitor In vivo Metabolic and Functional Alterations in Murine Myocardial Infarction

Cited by 6 publications

References 46 publications

4D ultrasound‐based strain assessment of cardiac dysfunction in male rats with reperfused and nonreperfused myocardial infarction

4D ultrasound‐based strain assessment of cardiac dysfunction in male rats with reperfused and nonreperfused myocardial infarction

Modulation of Rxrα Expression in Mononuclear Phagocytes Impacts on Cardiac Remodeling after Ischemia-Reperfusion Injury

Recent Advances in Cardiovascular Diseases Research Using Animal Models and PET Radioisotope Tracers

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