Cardiac and Metabolic Impact of Functional Foods with Antioxidant Properties Based on Whey Derived Proteins Enriched with Hemp Seed Oil

Abstract

The impaired ability to feed properly, evident in oncologic, elderly, and dysphagic patients, may result in malnutrition and sarcopenia. Increasing the consumption of dietary proteins by functional foods and enriching their composition by adding beneficial nutrients may represent an adjuvant therapy. We aimed to evaluate the safety and the positive effects of a standard diet (SD) supplemented with whey-derived protein puddings (WDPP), with appropriate rheological properties, and hemp seed oil (HSO), rich in polyphenols. Rats were assigned to SD, WDPP, WDPP plus hemp seed oil (HSOP), and HSO supplemented diets for eight weeks. “Anthropometric”, metabolic, and biochemical variables, oxidative stress, tissue injury, liver histology, and cardiac susceptibility to ischemia/reperfusion were analyzed. All the supplementations did not induce significant changes in biochemical and metabolic variables, also in relation to glucose tolerance, and livers did not undergo morphological alteration and injury. An improvement of cardiac post-ischemic function in the Langendorff perfused heart model and a reduction of infarct size were observed in WDPP and HSOP groups, thanks to their antioxidant effects and the activation of Akt- and AMPK-dependent protective pathways. Data suggest that (i) functional foods enriched with WDPP and HSOP may be used to approach malnutrition and sarcopenia successfully under disabling conditions, also conferring cardioprotection, and that (ii) adequate rheological properties could positively impact dysphagia-related problems.

Antioxidant Activity of Hemp (Cannabis sativa L.) Seed Oil in Drosophila melanogaster Larvae under Non-Stress and H2O2-Induced Oxidative Stress Conditions

“…After cooling to room temperature, 1 µL of the pyridine solution of TMS derivatives was injected. The GC-MS program used for this purpose was identical (70(0 )/5 • C min −1 /315 (30 )) to the one used to record the original sample prior to silylation, but also included a 7 min delay (the time that elapsed after the injection until the MS detector turn on).…”

“…Fotschki et al [ 29 ] reported that hemp seed oil induced oxidative stress in livers of obese rats, while in healthy rats it positively affected the antioxidant status. The use of whey-derived proteins enriched with hemp seed oil in the rats’ diet led to a reduction of oxidative stress marker in post-ischemic heart samples [ 30 ]. Toxic effects of copper in fish were overcome by application of dietary hemp seed oil, which was explained by a prooxidant–antioxidant balance at ω-3 and ω-6 fatty acid levels [ 31 ].…”

Importance of feeding status evaluation in older patients undergoing hemodialysis

“…Paste meals contain a relatively large amount of water to enable stirring and swallowing; consequently, substantial amounts of ingredients decreased, leading to reduced energy and protein intake. A previous study suggested that adding oil to food could be an alternative nutritional method for patients with dysphagia [ 35 ], as lipids are high in calories, and suitable for adjusting the rheological properties of foods [ 35 ]. Further studies are needed to verify the effect of adding oil to food.…”

Applications of Hemp Polymers and Extracts in Food, Textile and Packaging: A Review

“…Monoecious varieties of industrial hemp are more prevalent by plant breeding, particularly those cultivated in Europe. Due to its antioxidant and antimicrobial properties, it is also used as an active ingredient in commercial insect repellents and biopesticides [ 5 , 6 ]. Hemp has the highest Young’s modulus values of any natural fiber and is the strongest cellulose fibers.…”