Cardiac differentiation of bone-marrow-resident c-kit+ stem cells by L-carnitine increases through secretion of VEGF, IL6, IGF-1, and TGF-β as clinical agents in cardiac regeneration

Fathi, Ezzatollah; Farahzadi, Raheleh; Vietor, Ilja; Javanmardi, Sara

doi:10.1007/s12038-020-00063-0

Cited by 48 publications

(31 citation statements)

References 47 publications

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“…15 Among the somatic stem/progenitor cells studied, skeletal myoblasts are unable to transdifferentiate into CMs; 44 adipose-derived stem/progenitor cells usage in infarct heart reduces scar formation and improves perfusion and thus improves cardiac function, but does not lead to direct differentiation into CMs; 44 cardiac stem/progenitor cells may differentiate into CMs, but it is not easy to collect sufficient numbers of these cell types and their efficiency is usually low. 16 , 45 BM resident stem/progenitor cells are relatively easy to obtain, which is conducive to autologous transplantation for cell therapy. Because a few BM stem/progenitor cells can enter circulating blood, it does not rule out that some somatic stem cells including cardiac stem/progenitor cells are derived from BM stem/progenitor cells, and the BM stem/progenitor cell-based cell therapy is also to explore, using a pharmacological approach, whether and how the circulating BM resident stem/progenitor cells participate in myocardial regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…C-kit + HSCs have prominent cardiac recovery after acute myocardial infarction, 51 and in CM differentiation medium can differentiate toward CMs and secrete a variety of cytokines and growth factors. 16 , 17 In summary, among BM resident stem/progenitor cells, c-kit + cells seems to be the most well-defined CM-differentiated subpopulation, but the efficiency is low due to their multipotent differentiation potential (CMs, smooth muscle cells, and endothelial cells) 52 and poor survival and maintenance in the damaged heart tissue. 16 , 17…”

Section: Discussionmentioning

confidence: 99%

“…16 , 17 In summary, among BM resident stem/progenitor cells, c-kit + cells seems to be the most well-defined CM-differentiated subpopulation, but the efficiency is low due to their multipotent differentiation potential (CMs, smooth muscle cells, and endothelial cells) 52 and poor survival and maintenance in the damaged heart tissue. 16 , 17…”

Section: Discussionmentioning

confidence: 99%

“…Actually, several adult stem cell populations, including hematopoietic stem/progenitor cells, have been shown to possess cardiac regenerative potential, 13–18 although results from different research groups have appeared diverse. 15 In addition, some progress has been made in artificial trans-differentiation of cardiac fibroblasts into CMs.…”

Section: Introductionmentioning

confidence: 99%

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Nucleated red blood cells participate in myocardial regeneration in the toad Bufo Gargarizan Gargarizan

Hou

Zhao

et al. 2021

Exp Biol Med (Maywood)

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Heart regeneration is negligible in humans and mammals but remarkable in some ectotherms. Humans and mammals lack nucleated red blood cells (NRBCs), while ectotherms have sufficient NRBCs. This study used Bufo gargarizan gargarizan, a Chinese toad subspecies, as a model animal to verify our hypothesis that NRBCs participate in myocardial regeneration. NRBC infiltration into myocardium was seen in the healthy toad hearts. Heart needle-injury was used as an enlarged model of physiological cardiomyocyte loss. It recovered quickly and scarlessly. NRBC infiltration increased during the recovery. Transwell assay was done to in vitro explore effects of myocardial injury on NRBCs. In the transwell system, NRBCs could infiltrate into cardiac pieces and could transdifferentiate toward cardiomyocytes. Heart apex cautery caused approximately 5% of the ventricle to be injured to varying degrees. In the mildly to moderately injured regions, NRBC infiltration increased and myocardial regeneration started soon after the inflammatory response; the severely damaged region underwent inflammation, scarring, and vascularity before NRBC infiltration and myocardial regeneration, and recovered scarlessly in four months. NRBCs were seen in the newly formed myocardium. Enzyme-linked immunosorbent assay and Western blotting showed that the levels of tumor necrosis factor-α, interleukin- 1β, 6, and11, cardiotrophin-1, vascular endothelial growth factor, erythropoietin, matrix metalloproteinase- 2 and 9 in the serum and/or cardiac tissues fluctuated in different patterns during the cardiac injury-regeneration. Cardiotrophin-1 could induce toad NRBC transdifferentiation toward cardiomyocytes in vitro. Taken together, the results suggest that the NRBC is a cell source for cardiomyocyte renewal/regeneration in the toad; cardiomyocyte loss triggers a series of biological processes, facilitating NRBC infiltration and transition to cardiomyocytes. This finding may guide a new direction for improving human myocardial regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nucleated red blood cells participate in myocardial regeneration in the toad Bufo Gargarizan Gargarizan

Hou

Zhao

et al. 2021

Exp Biol Med (Maywood)

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“…AFP was measured by enzyme-linked immunosorbent assay (ELISA) (IMMULITE 1000 system by a kit provided by Siemens Medical Solutions Diagnostics, USA) [ 28 ]. Moreover, the ELISA technique was used to estimate the serum level of TGF-β1 (Human TGF-β ELISA Kit, SunRed, China) [ 29 ]. HCV RNA was detected by RT-PCR using COBAS TaqMan HCV quantitative test, version 2.0 (Roche Molecular Systems, Inc., Branchburg, NJ, USA) [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Inverse relationship between the level of miRNA 148a-3p and both TGF-β1 and FIB-4 in hepatocellular carcinoma

Dawood

Saleh

Elbahr

et al. 2021

Biochemistry and Biophysics Reports

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Background and aim Hepatocellular carcinoma (HCC) is a major health burden globally. Dysregulation of miRNA 148a-3p is engaged in carcinogenesis. TGF-β is a profibrogenic cytokine. This study assesses the expression level of miRNA 148a-3p and its relationship with serum TGF-β1 and fibrosis index based on four factors (FIB-4) in Egyptian patients with HCV-associated HCC. Subjects and Methods: The study included 72 HCC patients with HCV, 48 HCV cirrhotic patients, and 47 healthy controls. Serum TGF-β1 was assessed by ELISA and the expression of miRNA 148a-3p was measured by RT-PCR. Results Patients with HCC had lower plasma miRNA 148a-3p, higher serum TGF-β1, and higher FIB-4 levels than patients with cirrhosis and controls. miRNA 148a-3p discriminated HCC either from control (AUC: 0.997, 95.83% sensitivity, 85.11% specificity) or from cirrhosis (AUC: 0.943, 91.67% sensitivity, 81.25% specificity). Moreover, it distinguished metastatic from nonmetastatic patients (AUC: 0.800, 88.89% sensitivity, 60.0% specificity). The decreased miRNA 148a-3p and the increased TGF-β1 levels were related to distant metastasis, multinodular lesions, advanced TNM stage, and BCLC score (C). A negative correlation between miRNA 148a-3p and each of FIB-4 and TGF-β1 was detected. The decreased miRNA 148a-3p was associated with poor overall survival and poor progression-free survival. Conclusion An inverse relationship between miRNA 148a-3p and both TGF-β1 and FIB-4 was observed, which could be involved in HCC pathogenesis. Moreover, this miRNA is a potential diagnostic and prognostic biomarker for HCC.

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Stem Cell Applications in Cardiac Tissue Regeneration

Garza-Treviño

Quiroz-Reyes

Roacho-Pérez

et al. 2023

Cardiovascular Applications of Stem Cells

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Cardiac differentiation of bone-marrow-resident c-kit+ stem cells by L-carnitine increases through secretion of VEGF, IL6, IGF-1, and TGF-β as clinical agents in cardiac regeneration

Cited by 48 publications

References 47 publications

Nucleated red blood cells participate in myocardial regeneration in the toad Bufo Gargarizan Gargarizan

Nucleated red blood cells participate in myocardial regeneration in the toad Bufo Gargarizan Gargarizan

Inverse relationship between the level of miRNA 148a-3p and both TGF-β1 and FIB-4 in hepatocellular carcinoma

Stem Cell Applications in Cardiac Tissue Regeneration

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