2011
DOI: 10.2174/1875043501104010095
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Cardiac Differentiation of Human Wharton's Jelly Stem Cells – Experimental Comparison of Protocols

Abstract: Cardiomyoplasty represents a promising approach for the repair of the injured heart, but is hampered by the availability of appropriate cells. Mesenchymal stem cells derived from the human umbilical cord tissue (UCMSC) can be obtained in large amounts without medical intervention, exhibit self renewal and immunological naivity as well as multipotency. In the present study, different published protocols of cardiac differentiation designed for different stem cell types were compared to differentiate UCMSC into c… Show more

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Cited by 23 publications
(17 citation statements)
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“…After 21 days the adherent cells showed cardiomyogenic morphology and a slight spontaneous beating [108]. Hollweck et al (2011) described the effect of six different cardiac differentiation protocols on WJCs, assessing the efficiency of oxytocin with respect to 5-azacytidine [109]. Nevertheless, the use of enzyme isolation protocols may drastically induce damages on surface markers that could result in modification in phenotype characterization and regarding their response when transplanted in vivo.…”
Section: Human Umbilical Cord: the Hidden Po-tential Of A Discarded Tmentioning
confidence: 99%
“…After 21 days the adherent cells showed cardiomyogenic morphology and a slight spontaneous beating [108]. Hollweck et al (2011) described the effect of six different cardiac differentiation protocols on WJCs, assessing the efficiency of oxytocin with respect to 5-azacytidine [109]. Nevertheless, the use of enzyme isolation protocols may drastically induce damages on surface markers that could result in modification in phenotype characterization and regarding their response when transplanted in vivo.…”
Section: Human Umbilical Cord: the Hidden Po-tential Of A Discarded Tmentioning
confidence: 99%
“…Using specific in vitro protocols, WJ-MSC can be differentiated into glial cells and neurons [9], skeletal muscle cells [23], cardiomyocytes [24,25], endothelial cells [26][27][28], hepatocytes [29,30]. In addition, the expression of connexin 43 [16] suggested the possibility to differentiate WJ-MSC into cardiomyocytes or other cardiac resident cell types [31] or to be directly administered to correct conduction defects and other heart diseases [32][33][34].…”
Section: Wj-msc Phenotype and Differentiation Capacitymentioning
confidence: 99%
“…Although5-azacytidine is known as a routine agent for the induction of cardiogenic differentiation, but conflicting data about its efficacy has been reported [37]. On the other hand, oxytocin, which is another agent, has shown greater potential than that of 5-azacytidine,therefore, we selected oxytocin to increaseand improve the qualification of cardiomyocyte differentiation [26,38,39]. The dhUCM cells have shown some contractile proteins such as cardiac actin, cardiac troponin T and sarcomeric actin as well as connex in 43, which indicates the electrical coupling of them together remarkably (Fig.…”
Section: Discussionmentioning
confidence: 99%