Cardiac electrophysiology in mice: a matter of size

Kaese, Sven; Verheule, Sander

doi:10.3389/fphys.2012.00345

Cited by 158 publications

(140 citation statements)

References 287 publications

(383 reference statements)

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“…SNRT sinus node recovery time, SNRTc corrected sinus node recovery time, SNRT/SCL SNRT/sinus cycle length, AV interval atrioventricular interval, ARP atrial refractory period, VRP ventricular refractory period wild type and mutant mice. Three different modes of physical stress testing (two treadmill protocols and swimming) provoked similar maximal heart rates in both genotypes that were comparable to what has been published before [32]. In addition, sinus node recovery time tested by electrical stimulation was not altered in Panx1 -/-mice.…”

Section: Discussionsupporting

confidence: 68%

“…Again, there was no significant difference when comparing the two genotypes (Table 4). All parameters obtained in the two genotypes were in accordance with what has been reported in mice previously [32]. Finally, repeated atrial and ventricular burst stimulation with different cycle lengths before and after injection of isoprenaline was applied to provoke arrhythmia and test for atrial and ventricular vulnerability.…”

Section: Electrophysiological Studies In Panx1supporting

confidence: 65%

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Pannexin-1 Deficient Mice Have an Increased Susceptibility for Atrial Fibrillation and Show a QT-Prolongation Phenotype

Petric

Klein

Dannenberg

et al. 2016

Cell Physiol Biochem

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Background/Aims: Pannexin-1 (Panx1) is an ATP release channel that is ubiquitously expressed and coupled to several ligand-gated receptors. In isolated cardiac myocytes, Panx1 forms large conductance channels that can be activated by Ca²⁺ release from the sarcoplasmic reticulum. Here we characterized the electrophysiological function of these channels in the heart in vivo, taking recourse to mice with Panx1 ablation. Methods: Cardiac phenotyping of Panx1 knock-out mice (Panx1^-/-) was performed by employing a molecular, cellular and functional approach, including echocardiography, surface and telemetric ECG recordings with QT analysis, physical stress testing and quantification of heart rate variability. In addition, an in vivo electrophysiological study entailed programmed electrical stimulation using an intracardiac octapolar catheter. Results: Panx1 deficiency results in a higher incidence of AV-block, delayed ventricular depolarisation, significant prolongation of QT- and rate corrected QT-interval and a higher incidence of atrial fibrillation after intraatrial burst stimulation. Conclusion: Panx1 seems to play an important role in murine cardiac electrophysiology and warrants further consideration in the context of hereditary forms of atrial fibrillation.

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Section: Discussionsupporting

confidence: 68%

Section: Electrophysiological Studies In Panx1supporting

confidence: 65%

Pannexin-1 Deficient Mice Have an Increased Susceptibility for Atrial Fibrillation and Show a QT-Prolongation Phenotype

Petric

Klein

Dannenberg

et al. 2016

Cell Physiol Biochem

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“…The main findings of our Ecc analysis indicate that while the rodent heart has some clear differences compared to the human heart in aspects such as action potential repolarization and calcium handling [22,23,25], its mechanical response to the effect of various pacing modes seems to have important similarities to the large mammalian heart. While previous studies in the mouse heart have already demonstrated negative effects of acute RV pacing on Figure 6.…”

Section: Discussionmentioning

confidence: 91%

Speckle-Tracking Echocardiography Elucidates the Effect of Pacing Site on Left Ventricular Synchronization in the Normal and Infarcted Rat Myocardium

et al. 2013

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“…This interval was shown to be smaller than for humans. However, changes in the QT interval hardly present clinical repercussion in animals, as occurs in humans, such as situations of hypothermia or hypercalcemia [22].…”

Section: Discussionmentioning

confidence: 99%

Electrocardiographic Pattern of Agoutis (Dasyprocta prymnolopha) Chemically Contained by the Association of Ketamine and Xylazine

Filho

Pessoa

Rodrigues

et al. 2017

Acta Scientiae. Vet.

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Background:The agouti (Dasyprocta prymnolopha, Wagler, 1831) is an existing wild rodent in almost all of Brazil, used as a biological model in several scientific studies. Veterinary cardiology has showed great advances in the diagnostic area due to the possibility of cardiac evaluation by non-invasive methods. For the practice of scientific or handling procedures in wild animals, chemical containment is essential. Thus, it is important to know the effect of anesthetic protocols on the cardiovascular system, observed through complementary tests, such as the electrocardiogram. The objective of this study was to describe the computerized electrocardiographic tracing of agoutis chemically contained by the association of Ketamine and Xylazine. Materials, Methods & Results: Eighteen male and female clinically healthy animals, aged among 2 years, submitted to digital electrocardiographic examination, were used. The device used to obtain the tracing was the veterinary electrocardiograph (Electrocardiogram Acquisition Module for Computer) -Brazilian Electronic Technology (TEB). The analysis was always made from derivation II (DII). Chemical containment was performed by the combination of Ketamine and Xylazine, intramuscularly. The results showed that the heart rate for males was on average 113.25 bpm, while for females the value of 124.60 bpm was observed, and there was no significant difference between the genders (P > 0.05). The mean weight of males was 2.31 kg and for females 2.28 kg; there was no statistical difference for this variable (P > 0.05). For the QRS duration of 46.14 ± 5.05 ms (males) and 44.66 ± 5.94 ms (females) and PR interval of 79.94 ± 12.01ms (males) and 84.29 ± 12.37ms (females), there was no statistical difference (P > 0.05). The amplitude of the R wave of 0.42 ± 0.31 mV (males) and 0.36 ± 0.22 mV (females) showed no statistical difference (P > 0.05). The T wave showed itself both negative (10 animals) and positive (8 animals), with amplitude measured at 0.24 ± 0.16 mV for males and 0.25 ± 0.10 mV for females (P > 0.05). The anesthetic protocol was well tolerated by the animals of this experiment, and there were no episodes of arrhythmias during the time of their monitoring. Discussion: Wild animals, in general, need chemical containment, with a view to reducing stress during scientific and handling procedures. The protocols established in this work can be used in future experiments which require a longer handling time. The values found for heart rate (HR) of anesthetized agoutis were lower than those for non-anesthetized agoutis, proving the influence of the stress during retention and the depressant cardiovascular effect of the anesthetic drug used. The electrocardiographic parameters and the tracing morphology of the agoutis were like the results found for anesthetized small rodents. The QT interval was higher in males than in females, justifying the fact that it could be influenced by variations in HR, which also showed superior results for males. Taking into consideration the morphol...

show abstract

Cardiac electrophysiology in mice: a matter of size

Cited by 158 publications

References 287 publications

Pannexin-1 Deficient Mice Have an Increased Susceptibility for Atrial Fibrillation and Show a QT-Prolongation Phenotype

Pannexin-1 Deficient Mice Have an Increased Susceptibility for Atrial Fibrillation and Show a QT-Prolongation Phenotype

Speckle-Tracking Echocardiography Elucidates the Effect of Pacing Site on Left Ventricular Synchronization in the Normal and Infarcted Rat Myocardium

Electrocardiographic Pattern of Agoutis (Dasyprocta prymnolopha) Chemically Contained by the Association of Ketamine and Xylazine

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