Cardiac function and blood flow hemodynamics assessment of zebrafish (Danio rerio) using high-speed video microscopy

Benslimane, Fatiha; Zakaria, Zain Zaki; Shurbaji, Samar; Abdelrasool, Mahmoud Khatib Ali; Al-Badr, Mashael Ali H I; Absi, Enas Said Khalil Al; Yalcin, Huseyin C.

doi:10.1016/j.micron.2020.102876

Cited by 35 publications

(29 citation statements)

References 40 publications

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“…We measured several heart function/hemodynamics parameters, including cardiac output (CO), blood flow, blood velocity, vessel diameter, and heartbeat, by tracking red blood cells (RBC) movements in the DA and PCV from the trunk of the embryos. To do so, we used Danioscope (Noldus Information Technology Inc., Wageningen, The Netherlands), and we utilized MicroZebraLab blood flow from Viewpoint (version 3.4.4, Lyon, France) [ 26 , 27 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Water-Pipe Smoking on the Normal Development of Zebrafish

Zakaria

Aladwi

Benslimane

et al. 2021

IJERPH

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Background: Among all types of tobacco consumption, Water-Pipe Smoking (WPS) is the most widely used in the Middle East and second-most in several other countries. The effect of WPS on normal development is not yet fully understood, thus the aim of this study is to explore the acute toxicity effects of WPS extract on zebrafish larvae. Methods: In this study, we compared the effects of WPS smoke condensates at concentrations varying from 50 to 200 µg/mL on developmental, cardiac, and behavioural (neurotoxicity) functions. Gene expression patterns of cardiac biomarkers were also evaluated by RT-qPCR. Results: Exposing zebrafish embryos to 50, 100, 150 and 200 µg/mL WPS for three days did not affect the normal morphology of Zebrafish embryos, as the tail flicking, behavioural and locomotion assays did not show any change. However, WPS deregulated cardiac markers including atrial natriuretic peptide (ANP/NPPA) and brain natriuretic peptide (BNP/NPPB). Furthermore, it induced apoptosis in a dose-dependent manner. Conclusion: Our data demonstrate that WPS can significantly affect specific cardiac parameters during the normal development of zebrafish. Further investigations are necessary to elucidate the pathogenic outcome of WPS on different aspects of human life, including pregnancy.

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Section: Methodsmentioning

confidence: 99%

Effect of Water-Pipe Smoking on the Normal Development of Zebrafish

Zakaria

Aladwi

Benslimane

et al. 2021

IJERPH

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“…High-speed video microscopy movies of the heart and tail in 1000 frames per 10 seconds at 100X magnification were recorded for the primordial midbrain channels (PMBC) and the dorsal aorta (DA). The recorded movies were analyzed according to our previous protocols (47,48) using MicroZebraLab blood flow from Viewpoint (version 3.4.4, Lyon, France) and four cardiac parameters were calculated for each vessel: pulse, blood flow velocity, vessel diameter, and shear stress using the following formula: 𝝉 = 𝟒𝝁𝑽 𝒎𝒆𝒂𝒏 𝑫 µ: blood viscosity (dyne/cm 2 ), V: average blood velocity (µm/s), D: vessel diameter (µm) This experiment was performed multiple times, and data from three independent experiments are reported.…”

Section: Shear Stress Zebrafish Flow Parameter Measurementsmentioning

confidence: 99%

Cilia proteins are biomarkers of altered flow in the vasculature

Gupta¹,

Thirugnanam²,

Thamilarasan³

et al. 2022

JCI Insight

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Cilia, microtubule-based organelles that project from the apical luminal surface of endothelial cells (ECs), are widely regarded as a low flow-sensors. Previous reports suggest that upon high shear stress, cilia on the EC surface are lost, and more recent evidence suggests that deciliation -the physical removal of cilia from the cell surface -is a predominant mechanism for cilia loss in mammalian cells. Thus, we hypothesized that EC deciliation facilitated by changes in shear stress will manifest in increased abundance of cilia-related proteins in circulation. To test this hypothesis, we performed shear stress experiments that mimicked flow conditions from low to high shear stress in human primary cells and a zebrafish model system. In the primary cells, we showed that upon shear stress induction, indeed, ciliary fragments were observed in the effluent in vitro and effluents contained ciliary proteins normally expressed in both endothelial and epithelial cells. In zebrafish, upon shear stress induction, fewer ciliary-expressing ECs were observed. To test the translational relevance of these findings, we investigated our hypothesis using patient blood samples from sickle cell disease and found that plasma levels of ciliary proteins were elevated compared to healthy controls. Further, sickled red blood cells demonstrated high levels of ciliary protein (ARL13b) on their surface post-adhesion to brain ECs. Brain ECs post interaction with sickle RBCs show high reactive oxygen species (ROS) levels. Attenuating ROS levels in brain ECs decreases cilia protein levels on RBCs and rescues ciliary protein levels in brain ECs. Collectively, these data suggest that cilia and ciliary proteins in circulation are detectable under various altered flow conditions, which could serve as a surrogate biomarker of the damaged endothelium.

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“…To assess zebrafish cardiac function and blood flow hemodynamics, Benslimane and her colleagues have established high-speed microscopy with a combination of an analysis software protocol for practical image acquisition for studying zebrafish’s cardiovascular system. They have provided a detailed calculation of several cardiac parameters and expected ranges for these cardiac parameters [ 123 , 124 ]. Moreover, Benslimane et al, have adapted a single-mode mice Doppler echocardiography system to assess the cardiac function of normal or diseased zebrafish and to measure the flow velocities of adult zebrafish [ 125 ].…”

Section: Anti-cancer Efficacy Assessmentmentioning

confidence: 99%

Zebrafish as a Model for Anticancer Nanomedicine Studies

2021

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Nanomedicine is a new approach to fight against cancer by the development of anticancer nanoparticles (NPs) that are of high sensitivity, specificity, and targeting ability to detect cancer cells, such as the ability of Silica NPs in targeting epithelial cancer cells. However, these anticancer NPs require preclinical testing, and zebrafish is a useful animal model for preclinical studies of anticancer NPs. This model affords a large sample size, optical imaging, and easy genetic manipulation that aid in nanomedicine studies. This review summarizes the numerous advantages of the zebrafish animal model for such investigation, various techniques for inducing cancer in zebrafish, and discusses the methods to assess cancer development in the model and to test for the toxicity of the anticancer drugs and NPs. In addition, it summarizes the recent studies that used zebrafish as a model to test the efficacy of several different anticancer NPs in treating cancer.

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Cardiac function and blood flow hemodynamics assessment of zebrafish (Danio rerio) using high-speed video microscopy

Cited by 35 publications

References 40 publications

Effect of Water-Pipe Smoking on the Normal Development of Zebrafish

Effect of Water-Pipe Smoking on the Normal Development of Zebrafish

Cilia proteins are biomarkers of altered flow in the vasculature

Zebrafish as a Model for Anticancer Nanomedicine Studies

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