Cardiac function and lipid distribution in rats fed a high-fat diet: in vivo magnetic resonance imaging and spectroscopy

Nagarajan, Vijayasarathi; Gopalan, Venkatesh; Kaneko, Minoru; Angeli, Véronique; Gluckman, Peter D.; Richards, A. Mark; Kuchel, Philip W.; Velan, S. Sendhil

doi:10.1152/ajpheart.00478.2012

Cited by 19 publications

(13 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, it was shown that carnitine supplementation can be used to restore myocardial free carnitine levels, followed by normalization of total fatty acid content in LCAD knockout mice (208). In rats exposed to a high-fat diet, cardiac lipid accumulation has been shown, together with moderate diabetes and mild cardiac hypertrophy (109). The cardiac lipid accumulation may be an early indication of the cardiovascular pathophysiology associated with type 2 diabetes.…”

Section: Tgs: Results From Animal Studiesmentioning

confidence: 99%

See 1 more Smart Citation

MRS: a noninvasive window into cardiac metabolism

et al. 2015

View full text Add to dashboard Cite

A well-functioning heart requires a constant supply of a balanced mixture of nutrients to be used for the production of adequate amounts of adenosine triphosphate, which is the main energy source for most cellular functions. Defects in cardiac energy metabolism are linked to several myocardial disorders. MRS can be used to study in vivo changes in cardiac metabolism noninvasively. MR techniques allow repeated measurements, so that disease progression and the response to treatment or to a lifestyle intervention can be monitored. It has also been shown that MRS can predict clinical heart failure and death. This article focuses on in vivo MRS to assess cardiac metabolism in humans and experimental animals, as experimental animals are often used to investigate the mechanisms underlying the development of metabolic diseases. Various MR techniques, such as cardiac (31) P-MRS, (1) H-MRS, hyperpolarized (13) C-MRS and Dixon MRI, are described. A short overview of current and emerging applications is given. Cardiac MRS is a promising technique for the investigation of the relationship between cardiac metabolism and cardiac disease. However, further optimization of scan time and signal-to-noise ratio is required before broad clinical application. In this respect, the ongoing development of advanced shimming algorithms, radiofrequency pulses, pulse sequences, (multichannel) detection coils, the use of hyperpolarized nuclei and scanning at higher magnetic field strengths offer future perspective for clinical applications of MRS.

show abstract

Section: Tgs: Results From Animal Studiesmentioning

confidence: 99%

“…In rats exposed to a high‐fat diet, cardiac lipid accumulation has been shown, together with moderate diabetes and mild cardiac hypertrophy . The cardiac lipid accumulation may be an early indication of the cardiovascular pathophysiology associated with type 2 diabetes.…”

Section: Spectroscopy Of Cardiac Metabolismmentioning

confidence: 99%

MRS: a noninvasive window into cardiac metabolism

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Cardiac steatosis is also regarded a potential mechanism for dysfunction in obesity and diabetes [28]. Recent MRI and spectroscopic analysis of rats fed a high-fat diet indicated that an accumulation of lipid in the myocardium was associated with impaired cardiac function [29]. Indeed, derangements in lipid metabolism due to accumulation of myocardial triacylglycerol can impair cardiac function and performance [28, 30–34].…”

Section: Discussionmentioning

confidence: 99%

Rapid development of cardiac dysfunction in a canine model of insulin resistance and moderate obesity

et al. 2015

View full text Add to dashboard Cite

Aims/hypothesis The worldwide incidence of obesity and diabetes continues to rise at an alarming rate. A major cause of the morbidity and mortality associated with obesity and diabetes is heart disease, yet the mechanisms that lead to cardiovascular complications remain unclear. Methods We performed cardiac MRI to assess left ventricular morphology and function during the development of moderate obesity and insulin resistance in a well-established canine model (n=26). To assess the influence of dietary fat composition, we randomised animals to a traditional lard diet (rich in saturated and monounsaturated fat; n=12), a salmon oil diet (rich in polyunsaturated fat; n=8) or a control diet (n=6). Results High-fat feeding with lard increased bodyweight and fasting insulin and markedly reduced insulin sensitivity. Lard feeding also significantly reduced left ventricular function, evidenced by a worsening of circumferential strain and impairment in left ventricular torsion. High-fat feeding with salmon oil increased bodyweight; however, salmon oil feeding did not impair insulin sensitivity or cardiac function. Conclusions/interpretation These data emphasise the importance of dietary fat composition on both metabolic and cardiac function, and have important implications for the relationship between diet and health.

show abstract

“…Further studies are needed to understand these complex interactions between cardiac steatosis and all these factors. Finally, although intramyocellular and extramyocellular lipids can be distinguished in skeletal muscle and animal hearts, [35][36][37] at present we cannot reliably differentiate these 2 lipid pools in human myocardium using our methods, and therefore we measure total lipid levels. Further work is needed to develop tools that can reliably differentiate the intramyocellular lipids (IMCL) and extramyocellular lipids (EMCL) in the human heart, but at the moment this is technical research and not ready for clinical studies.…”

Section: Study Limitationsmentioning

confidence: 93%

Myocardial Steatosis and Left Ventricular Contractile Dysfunction in Patients With Severe Aortic Stenosis

Mahmod

Bull

Suttie

et al. 2013

Circ: Cardiovascular Imaging

View full text Add to dashboard Cite

Background-Aortic stenosis (AS) leads to left ventricular (LV) hypertrophy and dysfunction. We hypothesized that cardiac steatosis is involved in the pathophysiology and also assessed whether it is reversible after aortic valve replacement. Methods and Results-Thirty-nine patients with severe AS (symptomatic=25, asymptomatic=14) with normal LV ejection fraction and no significant coronary artery disease and 20 age-and sex-matched healthy controls underwent cardiac 1H-magnetic resonance spectroscopy and imaging for the determination of steatosis (myocardial triglyceride content) and cardiac function, including circumferential strain (measured by magnetic resonance tagging). Strain was lower in both symptomatic and asymptomatic AS (−16.4±2.5% and −18.1±2.9%, respectively, versus controls −20.7±2.0%, both P<0.05). Myocardial steatosis was found in both symptomatic and asymptomatic patients with AS (0.89±0.42% in symptomatic AS; 0.75±0.36% in asymptomatic AS versus controls 0.45±0.17, both P<0.05). Importantly, multivariable analysis indicated that steatosis was an independent correlate of impaired LV strain. Spectroscopic measurements of myocardial triglyceride content correlated significantly with histological analysis of biopsies obtained during aortic valve replacement. At 8.0±2.1 months after aortic valve replacement, steatosis and strain had recovered toward normal. Conclusions-Pronounced myocardial steatosis is present in severe AS, regardless of symptoms, and is independently associated with the degree of LV strain impairment. Myocardial triglyceride content measured by magnetic resonance spectroscopy correlates with histological quantification. Steatosis and strain impairment are reversible after aortic valve replacement. Our findings suggest a novel pathophysiological mechanism in AS, myocardial steatosis, which may be amenable to treatment, thus potentially delaying onset of LV dysfunction.

show abstract

Cardiac function and lipid distribution in rats fed a high-fat diet: in vivo magnetic resonance imaging and spectroscopy

Cited by 19 publications

References 50 publications

MRS: a noninvasive window into cardiac metabolism

MRS: a noninvasive window into cardiac metabolism

Rapid development of cardiac dysfunction in a canine model of insulin resistance and moderate obesity

Myocardial Steatosis and Left Ventricular Contractile Dysfunction in Patients With Severe Aortic Stenosis

Contact Info

Product

Resources

About