Cardiac neural crest contributes to cardiomyogenesis in zebrafish

Sato, Mariko; Yost, H. Joseph

doi:10.1016/s0012-1606(03)00037-x

Cited by 118 publications

(122 citation statements)

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“…2K,K′). In zebrafish, by means of cell transplantation and different labeling techniques, a NC contribution to cardiomyocytes has already been demonstrated (Sato and Yost, 2003;Li et al, 2003). We also observed clones in the adrenomedullar region (Fig.…”

Section: Nc-derived Structures In the Trunksupporting

confidence: 56%

Genetic lineage labeling in zebrafish uncovers novel neural crest contributions to the head, including gill pillar cells

et al. 2013

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SUMMARYAt the protochordate-vertebrate transition, a new predatory lifestyle and increased body size coincided with the appearance of a true head. Characteristic innovations of this head are a skull protecting and accommodating a centralized nervous system, a jaw for prey capture and gills as respiratory organs. The neural crest (NC) is a major ontogenetic source for the 'new head' of vertebrates and its contribution to the cranial skeleton has been intensively studied in different model organisms. However, the role of NC in the expansion of the respiratory surface of the gills has been neglected. Here, we use genetic lineage labeling to address the contribution of NC to specific head structures, in particular to the gills of adult zebrafish. We generated a sox10:ER T2 -Cre line and labeled NC cells by inducing Cre/loxP recombination with tamoxifen at embryonic stages. In juvenile and adult fish, we identified numerous established NC derivatives and, in the cranium, we precisely defined the crest/mesoderm interface of the skull roof. We show the NC origin of the opercular bones and of multiple cell types contributing to the barbels, chemosensory organs located in the mouth region. In the gills, we observed labeled primary and secondary lamellae. Clonal analysis reveals that pillar cells, a craniate innovation that mechanically supports the filaments and forms gill-specific capillaries, have a NC origin. Our data point to a crucial role for the NC in enabling more efficient gas exchange, thus uncovering a novel, direct involvement of this embryonic tissue in the evolution of respiratory systems at the protochordate-vertebrate transition.

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Section: Nc-derived Structures In the Trunksupporting

confidence: 56%

Genetic lineage labeling in zebrafish uncovers novel neural crest contributions to the head, including gill pillar cells

et al. 2013

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“…These figures, which are based on the assumption that we have labeled only one cardiac progenitor per experiment, are likely to be underestimates. Additional cardiac progenitors could reside in areas outside of the scope of our fate map, such as deeper layers of the DEL, or regions outside the LMZs, such as cardiac neural crest (Li et al, 2003;Sato and Yost, 2003).…”

Section: Discussionmentioning

confidence: 99%

Organization of cardiac chamber progenitors in the zebrafish blastula

2004

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Organogenesis requires the specification of a variety of cell types and the organization of these cells into a particular three-dimensional configuration. The embryonic vertebrate heart is organized into two major chambers, the ventricle and atrium, each consisting of two tissue layers, the myocardium and endocardium. The cellular and molecular mechanisms responsible for the separation of ventricular and atrial lineages are not well understood. To test models of cardiac chamber specification, we generated a high-resolution fate map of cardiac chamber progenitors in the zebrafish embryo at 40% epiboly, a stage prior to the initiation of gastrulation. Our map reveals a distinct spatial organization of myocardial progenitors: ventricular myocardial progenitors are positioned closer to the margin and to the dorsal midline than are atrial myocardial progenitors. By contrast, ventricular and atrial endocardial progenitors are not spatially organized at this stage. The relative orientations of ventricular and atrial myocardial progenitors before and after gastrulation suggest orderly movements of these populations. Furthermore, the initial positions of myocardial progenitors at 40% epiboly indicate that signals residing at the embryonic margin could influence chamber fate assignment. Indeed, via fate mapping, we demonstrate that Nodal signaling promotes ventricular fate specification near the margin, thereby playing an important early role during myocardial patterning.

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“…Besides the contribution of neural crest cells to the parasympathetic ganglia of the heart, they are required to form the aortico-pulmonary septum dividing the cardiac arterial pole into the ascending aorta and the pulmonary trunk and also they provide signals required for the maintenance and differentiation of the other cell layers in the pharyngeal arches (Brown and Baldwin, 2006;Hutson and Kirby, 2007). In zebrafish, even myocardial differentiation of cardiac neural crest cells has been described (Li et al, 2003;Sato and Yost, 2003). Recently, cardiac neural crest cells have been shown to modulate signalling in the pharynx during the lengthening of the outflow tract, and in fact the neural crest is required for the addition of new myocardium to the outflow tract from the secondary heart field (Waldo et al, 2005;Hutson et al, 2006).…”

Section: The Contribution Of the Neural Crestmentioning

confidence: 99%

Building the vertebrate heart - an evolutionary approach to cardiac development

2009

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The vertebrate heart is unique among the blood pumps described in metazoans. In contrast to the myoepithelial tubes found in most animal phyla, the vertebrate heart is made up of multilayered myocardial cells surrounded by connective tissue derived from epicardium and endocardium, and endowed with complex valvular, coronary vessel and conduction systems. Despite these profound differences, a common genetic program seems to underlie the specification and differentiation of all the cardiac tissues. In this article, we will review the similarities in the transcriptional networks and signalling mechanisms regulating cardiac development in different animals, as well as the origin of the main differences existing between vertebrate and invertebrate hearts. We will pay special attention to the hypotheses concerning the evolutionary origin of the endothelium and the epicardium from ancestral blood cells and pronephric progenitors, respectively. We can summarize the transition between the invertebrate and the vertebrate heart as the result of the thickening of the primarily myoepithelial cardiac tube which was concomitant with: 1) an inner lining by an endothelium with the ability to transform into mesenchyme; 2) an outer lining derived from an ancestral pronephric glomerular primordium with vasculogenic potential; 3) a neural crest cell population which reaches the heart from the pharyngeal region; 4) the incorporation of new myocardium at both ends from a second heart field and 5) the formation of specialized chambers. The complex interactions between all these elements originated an exceptionally powerful blood pump which allowed vertebrates to reach their characteristically large size and activity.

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Cardiac neural crest contributes to cardiomyogenesis in zebrafish

Cited by 118 publications

References 50 publications

Genetic lineage labeling in zebrafish uncovers novel neural crest contributions to the head, including gill pillar cells

Genetic lineage labeling in zebrafish uncovers novel neural crest contributions to the head, including gill pillar cells

Organization of cardiac chamber progenitors in the zebrafish blastula

Building the vertebrate heart - an evolutionary approach to cardiac development

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