Cardiac repair achieved by bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid patches in a rat of myocardial infarction model

Hsin, Nai; Ming, Yang; Chung, Tsair-Wang; Chen, Jia Yu; Chou, Nai‐Kuan; Wang, Shoei‐Shen

doi:10.1016/j.biomaterials.2012.04.030

Cited by 94 publications

(72 citation statements)

References 33 publications

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“…Polymeric matrices aim at providing a favorable environment for ex vivo cell adhesion and growth, and for facilitated implantation onto the ischemic myocardium [4,[8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Plasma-functionalized electrospun matrix for biograft development and cardiac function stabilization

et al. 2014

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Cardiac tissue engineering approaches can deliver large numbers of cells to the damaged myocardium and have thus increasingly been considered as a possible curative treatment to counteract the high prevalence of progressive heart failure after myocardial infarction (MI). Optimal scaffold architecture and mechanical and chemical properties, as well as immune-and bio-compatibility, need to be addressed. We demonstrated that radio-frequency plasma surface functionalized electrospun poly (e-caprolactone) (PCL) fibres provide a suitable matrix for bone-marrow-derived mesenchymal stem cell (MSC) cardiac implantation. Using a rat model of chronic MI, we showed that MSC-seeded plasma-coated PCL grafts stabilized cardiac function and attenuated dilatation. Significant relative decreases of 13% of the ejection fraction (EF) and 15% of the fractional shortening (FS) were observed in sham treated animals; respective decreases of 20% and 25% were measured 4 weeks after acellular patch implantation, whereas a steadied function was observed 4 weeks after MSC-patch implantation (relative decreases of 6% for both EF and FS).

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“…Polymeric matrices aim at providing a favorable environment for ex vivo cell adhesion and growth, and for facilitated implantation onto the ischemic myocardium [4,[8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Plasma-functionalized electrospun matrix for biograft development and cardiac function stabilization

et al. 2014

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“…Similar beneficial therapeutic performance of cell-laden patches has been shown in several other studies. Chi et al [30] showed that the incorporation of mesenchymal stem cells markedly improved LV remodeling and myocardial viability compared to treatment of silk fibrin/hyaluronic acid patch alone, while added systolic contractility was noted by Qiuan et al [31] in epicardially grafted cell patches.…”

Section: Discussionmentioning

confidence: 96%

Epicardial application of cardiac progenitor cells in a 3D-printed gelatin/hyaluronic acid patch preserves cardiac function after myocardial infarction

et al. 2015

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“…Fibrin glue is known in both the laboratory and clinical settings thanks to its ability to quickly aid in clot formation, as well as its potential to deliver and host MSCs [14][15][16][17]19 . For our purposes, fibrin glue served as a biocompatible approach to adhering a water-based hydrogel construct to the surface of the heart.…”

Section: Discussionmentioning

confidence: 99%

A Hydrogel Construct and Fibrin-based Glue Approach to Deliver Therapeutics in a Murine Myocardial Infarction Model.

Melhem

Jensen

Reinkensmeyer

et al. 2015

JoVE

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The murine MI model is widely recognized in the field of cardiovascular disease, and has consistently been used as a first step to test the efficacy of treatments in vivo 1 . The traditional, established protocol has been further fine-tuned to minimize the damage to the animal. Notably, the pectoral muscle layers are teased away rather than simply cut, and the thoracotomy is approached intercostally as opposed to breaking the ribs in a sternotomy, preserving the integrity of the ribcage. With these changes, the overall stress on the animal is decreased.Stem cell therapies aimed to alleviate the damage caused by MIs have shown promise over the years for their pro-angiogenic and anti-apoptotic benefits. Current approaches of delivering cells to the heart surface typically involve the injection of the cells either near the damaged site, within a coronary artery, or into the peripheral blood stream [2][3][4] . While the cells have proven to home to the damaged myocardium, functionality is limited by their poor engraftment at the site of injury, resulting in diffusion into the blood stream 5 . This manuscript highlights a procedure that overcomes this obstacle with the use of a cell-encapsulated hydrogel patch. The patch is fabricated prior to the surgical procedure and is placed on the injured myocardium immediately following the occlusion of the left coronary artery. To adhere the patch in place, biocompatible external fibrin glue is placed directly on top of the patch, allowing for it to dry to both the patch and the heart surface. This approach provides a novel adhesion method for the application of a delicate cell-encapsulating therapeutic construct. Video LinkThe video component of this article can be found at

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Cardiac repair achieved by bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid patches in a rat of myocardial infarction model

Cited by 94 publications

References 33 publications

Plasma-functionalized electrospun matrix for biograft development and cardiac function stabilization

Plasma-functionalized electrospun matrix for biograft development and cardiac function stabilization

Epicardial application of cardiac progenitor cells in a 3D-printed gelatin/hyaluronic acid patch preserves cardiac function after myocardial infarction

A Hydrogel Construct and Fibrin-based Glue Approach to Deliver Therapeutics in a Murine Myocardial Infarction Model.

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