Cardiac Unloading Alters Contractility and Calcium Homeostasis in Ventricular Myocytes

Ritter, Michael; Su, Zhi; Xu, Shixuan; Shelby, Jane; Barry, William H.

doi:10.1006/jmcc.2000.1101

Cited by 25 publications

(31 citation statements)

References 21 publications

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“…This result was similar to other studies using the same model as the normal heart. [22][23][24] Consistent with our results, clinical studies have demonstrated that excessive or prolonged ventricular unloading with an LVAD causes disuse atrophy of heart muscles. This atrophic change is thought to impede functional recovery.…”

Section: Reversal Of Morphologic Hypertrophysupporting

confidence: 95%

Determination of Optimal Duration of Mechanical Unloading for Failing Hearts to Achieve Bridge to Recovery in a Rat Heterotopic Heart Transplantation Model

Oriyanhan

Tsuneyoshi

Nishina

et al. 2007

The Journal of Heart and Lung Transplantation

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Section: Reversal Of Morphologic Hypertrophysupporting

confidence: 95%

Determination of Optimal Duration of Mechanical Unloading for Failing Hearts to Achieve Bridge to Recovery in a Rat Heterotopic Heart Transplantation Model

Oriyanhan

Tsuneyoshi

Nishina

et al. 2007

The Journal of Heart and Lung Transplantation

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“…Studies with shorter periods of unloading were not associated with contractile dysfunction. 14, 30 Oriyanhan et al recently showed a time-dependent reduction of hypertrophied cardiomyocyte size and papillary muscle contractile function after heterotopic abdominal transplantation of failing rat hearts, suggesting prolonged unloading may have detrimental functional consequences. 17 Maybaum et al reported an improvement in LV function (LV ejection fraction Ͼ40%) in heart failure patients after 30 days of LVAD support.…”

Section: Unloading-induced Atrophymentioning

confidence: 98%

Prolonged Mechanical Unloading Reduces Myofilament Sensitivity to Calcium and Sarcoplasmic Reticulum Calcium Uptake Leading to Contractile Dysfunction

Soppa

Lee

Stagg

et al. 2008

The Journal of Heart and Lung Transplantation

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“…In addition, in the current study we observed that the transplanted neonatal heart developed a rather thickened LV wall after 2 weeks of mechanical unloading. This might be another unique feature of immaturity as it is well known that adult hearts become atrophic under prolonged mechanical unloading [19,20,21]. Further analyses of the current models can potentially characterize the age-dependent differences in myocardial response to mechanical unloading.…”

Section: Discussionmentioning

confidence: 99%

Development of a Vascularized Heterotopic Neonatal Rat Heart Transplantation Model

Shimada

Nido²,

Friehs³

2016

Eur Surg Res

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Background/Purpose: Rodent adult-to-adult heterotopic heart transplantation is a well-established animal model, and the detailed surgical technique with several modifications has been previously described. In immature donor organ transplantation, however, the surgical technique needs to be revised given the smaller size and fragility of the donor graft. Here, we report our surgical technique for heterotopic abdominal (AHTx) and femoral (FHTx) neonatal rat heart transplantation based on an experience of over 300 cases. Methods: Heterotopic heart transplantation was conducted in syngeneic Lewis rats. Neonatal rats (postnatal day 2-4) served as donors. AHTx was performed by utilizing the conventional adult-to-adult transplant method with specific modifications for optimal aortotomy and venous anastomosis. In the FHTx, the donor heart was vascularized by connecting the donor's aorta and pulmonary artery to the recipient's right femoral artery and vein, respectively, in an end-to-end manner. A specifically fashioned butterfly-shaped rubber sheet was used to align the target vessels properly. The transplanted graft was visually assessed for its viability and was accepted as a technical success when the viability met specific criteria. Successfully transplanted grafts were subject to further postoperative evaluation. Forty cases (AHTx and FHTx; n = 20 each) were compared regarding perioperative parameters and outcomes. Results: Both models were technically feasible (success rate: AHTx 75% vs. FHTx 70%) by refining the conventional heterotopic transplant technique. Injury to the fragile donor aorta and congestion of the graft due to suboptimal venous connection were predominant causes of failure, leading to refractory bleeding and poor graft viability. Although the FHTx required significantly longer operation time and graft ischemic time, the in situ graft viabilities were comparable. The FHTx provided better postoperative monitoring as it enabled daily graft palpation and better echocardiographic visualization. Conclusions: We describe detailed surgical techniques for AHTx and FHTx while addressing neonatal donor-specific issues. Following our recommendations potentially reduces the learning curve to achieve reliable and reproducible results with these challenging animal models.

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Cardiac Unloading Alters Contractility and Calcium Homeostasis in Ventricular Myocytes

Cited by 25 publications

References 21 publications

Determination of Optimal Duration of Mechanical Unloading for Failing Hearts to Achieve Bridge to Recovery in a Rat Heterotopic Heart Transplantation Model

Determination of Optimal Duration of Mechanical Unloading for Failing Hearts to Achieve Bridge to Recovery in a Rat Heterotopic Heart Transplantation Model

Prolonged Mechanical Unloading Reduces Myofilament Sensitivity to Calcium and Sarcoplasmic Reticulum Calcium Uptake Leading to Contractile Dysfunction

Development of a Vascularized Heterotopic Neonatal Rat Heart Transplantation Model

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