Cardinal Orientation Selectivity Is Represented by Two Distinct Ganglion Cell Types in Mouse Retina

Nath, Amurta; Schwartz, Greg

doi:10.1523/jneurosci.4554-15.2016

Cited by 81 publications

(105 citation statements)

References 58 publications

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“…We also measured the longest axis through a polygon outlining the dendritic field in each stratum (Figure S1A). The long axes were only ~40% longer than the equivalent diameter, a sign of symmetric morphology shared by most RGCs [16–18]. We found OND RGCs in locations throughout the retina with no apparent gradient in density, function or morphology.…”

Section: Resultsmentioning

confidence: 71%

“…In fact, OND RGCs had surprisingly large receptive fields (maximal response diameter, 386 ± 30 μm, n = 16) given their dendritic diameter. Unlike most RGC types ([7,16,25]; Figure 2A), OND RGCs showed very little surround suppression.…”

Section: Resultsmentioning

confidence: 99%

“…OND RGCs stratified near the ON choline acetyl-transferase (ChAT) band and distal to the OFF ChAT band (Figure 1G). This represents another example, along with the M1 ipRGC [15] and the ON OS RGC [16] of a functionally pure ON RGC with an OFF dendritic stratification. We confirmed that OND RGCs retained pure ON polarity in photopic conditions (Figure S2).…”

Section: Resultsmentioning

confidence: 99%

“…Electrophysiology experiments were performed on retinas from wild-type mice presented with visual stimuli as described previously [16,22]. Methodological details, including data and image analysis and modeling can be found in Supplementary Experimental Procedures.…”

Section: Methodsmentioning

confidence: 99%

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Circuit Mechanisms of a Retinal Ganglion Cell with Stimulus-Dependent Response Latency and Activation Beyond Its Dendrites

Mani

Schwartz

2017

Current Biology

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SUMMARY Center-surround antagonism has been used as the canonical model to describe receptive fields of retinal ganglion cells (RGCs) for decades. We describe a newly identified RGC type in mouse, called the ON delayed (OND) RGC, with receptive field properties that deviate from center-surround organization. Responding with an unusually long latency to light stimulation, OND RGCs respond earlier as the visual stimulus increases in size. Furthermore, OND RGCs are excited by light falling far beyond their dendrites. We unravel details of the circuit mechanisms behind these phenomena, revealing new roles for inhibition in controlling both temporal and spatial receptive field properties. The non-canonical receptive field properties of the OND RGC – integration of long temporal and large spatial scales – suggest that unlike typical RGCs, it may encode a slowly varying, global property of the visual scene.

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Section: Resultsmentioning

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Circuit Mechanisms of a Retinal Ganglion Cell with Stimulus-Dependent Response Latency and Activation Beyond Its Dendrites

Mani

Schwartz

2017

Current Biology

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“…Our 73 corresponds to the On delayed cell as defined by Mani and Schwartz (2017). Our 82wi corresponds to the vertically orientation selective cell studied by Nath and Schwartz (2016). Some of the confirmed types can also be found in the survey of Helmstaedter et al (2013).…”

Section: Resultsmentioning

confidence: 86%

Digital Museum of Retinal Ganglion Cells with Dense Anatomy and Physiology

Bae

Kim

et al. 2018

Cell

231

244

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When 3D electron microscopy and calcium imaging are used to investigate the structure and function of neural circuits, the resulting datasets pose new challenges of visualization and interpretation. Here, we present a new kind of digital resource that encompasses almost 400 ganglion cells from a single patch of mouse retina. An online "museum" provides a 3D interactive view of each cell's anatomy, as well as graphs of its visual responses. The resource reveals two aspects of the retina's inner plexiform layer: an arbor segregation principle governing structure along the light axis and a density conservation principle governing structure in the tangential plane. Structure is related to visual function; ganglion cells with arbors near the layer of ganglion cell somas are more sustained in their visual responses on average. Our methods are potentially applicable to dense maps of neuronal anatomy and physiology in other parts of the nervous system.

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A novel map of the mouse eye for orienting retinal topography in anatomical space

Stabio

Sondereker

Haghgou

et al. 2018

J of Comparative Neurology

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Functionally distinct retinal ganglion cells have density and size gradients across the mouse retina, and some degenerative eye diseases follow topographic-specific gradients of cell death. Hence, the anatomical orientation of the retina with respect to the orbit and head is important for understanding the functional anatomy of the retina in both health and disease. However, different research groups use different anatomical landmarks to determine retinal orientation (dorsal, ventral, temporal, nasal poles). Variations in the accuracy and reliability in marking these landmarks during dissection may lead to discrepancies in the identification and reporting of retinal topography. The goal of this study was to compare the accuracy and reliability of the canthus, rectus muscle, and choroid fissure landmarks in reporting retinal orientation. The retinal relieving cut angle made from each landmark during dissection was calculated based on its relationship to the opsin transition zone (OTZ), determined via a custom MATLAB script that aligns retinas from immunostained s-opsin. The choroid fissure and rectus muscle landmarks were the most accurate and reliable, while burn marks using the canthus as a reference were the least. These values were used to build an anatomical map that plots various ocular landmarks in relationship to one another, to the horizontal semicircular canals, to lambda-bregma, and to the earth's horizon. Surprisingly, during normal locomotion, the mouse's opsin gradient and the horizontal semicircular canals make equivalent 6° angles aligning the OTZ near the earth's horizon, a feature which may enhance the mouse's ability to visually navigate through its environment.

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Cardinal Orientation Selectivity Is Represented by Two Distinct Ganglion Cell Types in Mouse Retina

Cited by 81 publications

References 58 publications

Circuit Mechanisms of a Retinal Ganglion Cell with Stimulus-Dependent Response Latency and Activation Beyond Its Dendrites

Circuit Mechanisms of a Retinal Ganglion Cell with Stimulus-Dependent Response Latency and Activation Beyond Its Dendrites

Digital Museum of Retinal Ganglion Cells with Dense Anatomy and Physiology

A novel map of the mouse eye for orienting retinal topography in anatomical space

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