Cardiolipin-incorporated liposomes with surface CRM197 for enhancing neuronal survival against neurotoxicity

Kuo, Yung‐Chih; Liu, Yuchuan

doi:10.1016/j.ijpharm.2014.07.003

Cited by 16 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have indicated that intracerebroventricular injection of Aβ 1–42 oligomers at a nanomolar dose can dramatically impair the learning and memory function in rats (28), accompanied by increased apoptosis in the hippocampus and high levels of pro-inflammation cytokines in the brain (29,30). Furthermore, intracerebroventricular injection of low doses of Aβ 25–35 (31) or Aβ 1–28 (32,33) has also been shown to influence the spatial memory function and Y-maze alternation behavior in mice.…”

Section: Discussionmentioning

confidence: 99%

Asiaticoside ameliorates β-amyloid-induced learning and memory deficits in rats by inhibiting mitochondrial apoptosis and reducing inflammatory factors

Zhang

et al. 2016

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. The present study aimed to investigate the effects of asiaticoside (AS) on the pathology and associated mechanisms of β-amyloid (Aβ)-induced Alzheimer's disease (AD) in rats. An AD rat model was established by lateral intracerebroventricular injection of Aβ 1-42 oligomers. Learning and memory function were evaluated by Morris water maze (MWM) test. In addition, hematoxylin and eosin (H&E) staining, transmission electron microscopy (TEM), immunohistochemistry, ELISA and western blot analysis were performed to evaluate the disease pathogenesis. The results indicated that AS exerted protective effects in rats treated with Aβ oligomers, in a dose-dependent manner, as evidenced by the improved learning and memory function in the MWM test. In addition, H&E staining of hippocampal tissue showed that the histological structure was damaged in the model group, which was restored by AS treatment. Aβ deposition was dramatically increased in the model group, and the pathological changes were reversed by AS treatment. TEM revealed that the subcellular structure was injured by Aβ oligomers, however, the structure was ameliorated by AS treatment. Furthermore, AS was found to reduce the elevated levels of pro-inflammatory cytokines, interleukin-6 and tumor necrosis factor-α, in the brains of Aβ-treated rats. In addition, AS treatment resulted in a significant decrease in the expression of caspases-3, whereas the expression of B-cell lymphoma-2 was significantly increased, in these Aβ-treated rats. According to the findings of the observed study, AS has a marked protective effect on Aβ-induced AD pathology, and the underlying mechanism may be associated with the alleviation of the mitochondrial injuries, the anti-inflammatory activities, and the influence on the expression levels of apoptosis-associated proteins.

show abstract

Section: Discussionmentioning

confidence: 99%

Asiaticoside ameliorates β-amyloid-induced learning and memory deficits in rats by inhibiting mitochondrial apoptosis and reducing inflammatory factors

Zhang

et al. 2016

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

“…Here, particular attention has been given to the diphtheria toxin receptor (DTR) and the Human Immunodeficiency Virus (HIV)-TAT proteins. A mutant of DTR with no toxicity or immunogenicity has been tested to transport nano-liposomes and polybutylcyanoacrylate NPs across the BBB, both in vitro and in vivo , and indeed, only grafted NPs were able to transcytose the barrier (van Rooy et al, 2011 ; Kuo and Chung, 2012 ; Kuo and Liu, 2014 ). The same strategy was successful when using a derivate of the HIV-TAT protein, linked to the surface of polymeric micelles or SiO 2 NPs through polyethylene glycol (PEG) molecules (Liu et al, 2008a , b ; Zhao X. et al, 2016 ).…”

Section: How To Reach the Brain: G-based Nanocarriers And The Blood-bmentioning

confidence: 99%

Interfacing Graphene-Based Materials With Neural Cells

Bramini

Alberini

Colombo

et al. 2018

Front. Syst. Neurosci.

109

114

View full text Add to dashboard Cite

The scientific community has witnessed an exponential increase in the applications of graphene and graphene-based materials in a wide range of fields, from engineering to electronics to biotechnologies and biomedical applications. For what concerns neuroscience, the interest raised by these materials is two-fold. On one side, nanosheets made of graphene or graphene derivatives (graphene oxide, or its reduced form) can be used as carriers for drug delivery. Here, an important aspect is to evaluate their toxicity, which strongly depends on flake composition, chemical functionalization and dimensions. On the other side, graphene can be exploited as a substrate for tissue engineering. In this case, conductivity is probably the most relevant amongst the various properties of the different graphene materials, as it may allow to instruct and interrogate neural networks, as well as to drive neural growth and differentiation, which holds a great potential in regenerative medicine. In this review, we try to give a comprehensive view of the accomplishments and new challenges of the field, as well as which in our view are the most exciting directions to take in the immediate future. These include the need to engineer multifunctional nanoparticles (NPs) able to cross the blood-brain-barrier to reach neural cells, and to achieve on-demand delivery of specific drugs. We describe the state-of-the-art in the use of graphene materials to engineer three-dimensional scaffolds to drive neuronal growth and regeneration in vivo, and the possibility of using graphene as a component of hybrid composites/multi-layer organic electronics devices. Last but not least, we address the need of an accurate theoretical modeling of the interface between graphene and biological material, by modeling the interaction of graphene with proteins and cell membranes at the nanoscale, and describing the physical mechanism(s) of charge transfer by which the various graphene materials can influence the excitability and physiology of neural cells.

show abstract

“…25 For the viability assay, the steps used to treat SK-N-MC cells were analogous to those for HBMECs and HAs described in the "Toxicity to HBMECs and HAs" section, except for the addition of 15 μM of fibrillar Aβ The protein content in the sample was determined using an ELISA spectrophotometer at 595 nm. The sample was heated to 95°C and added to a 25% (v/v) sample buffer containing 25 μg of protein per vial.…”

Section: Viability Of Sk-n-mc Cells After Treatment With Rmp-7-lf-qu-lsmentioning

confidence: 99%

Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin

Kuo

Tsao

2017

IJN

Self Cite

View full text Add to dashboard Cite

A drug delivery system of quercetin (QU)-encapsulated liposomes (LS) grafted with RMP-7, a bradykinin analog, and lactoferrin (Lf) was developed to permeate the blood–brain barrier (BBB) and rescue degenerated neurons, acting as an Alzheimer’s disease (AD) pharmacotherapy. This colloidal formulation of QU-encapsulated LS grafted with RMP-7 and Lf (RMP-7-Lf-QU-LS) was used to traverse human brain microvascular endothelial cells (HBMECs) regulated by human astrocytes (HAs) and to treat SK-N-MC cells after an insult with cytotoxic β-amyloid (Aβ) fibrils. We found that surface RMP-7 and Lf enhanced the ability of QU to cross the BBB without inducing strong toxicity and damaging the tight junction. In addition, RMP-7-Lf-QU-LS significantly reduced Aβ-induced neurotoxicity and improved the viability of SK-N-MC cells. Compared with free QU, RMP-7-Lf-QU-LS could also significantly inhibit the expression of phosphorylated c-Jun N terminal kinase, phosphorylated p38, and phosphorylated tau protein at serine 202 by SK-N-MC cells, indicating an important role of RMP-7, Lf, and LS in protecting neurons against apoptosis. RMP-7-Lf-QU-LS is a promising carrier targeting the BBB to prevent Aβ-insulted neurodegeneration and may have potential in managing AD in future clinical applications.

show abstract

Cardiolipin-incorporated liposomes with surface CRM197 for enhancing neuronal survival against neurotoxicity

Cited by 16 publications

References 35 publications

Asiaticoside ameliorates β-amyloid-induced learning and memory deficits in rats by inhibiting mitochondrial apoptosis and reducing inflammatory factors

Asiaticoside ameliorates β-amyloid-induced learning and memory deficits in rats by inhibiting mitochondrial apoptosis and reducing inflammatory factors

Interfacing Graphene-Based Materials With Neural Cells

Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin

Contact Info

Product

Resources

About