Cardioprotection of salvianolic acid B and ginsenoside Rg1 combination on subacute myocardial infarction and the underlying mechanism

Li, Yuanmin; Wang, Linlin; Dong, Zhihui; Wang, Shiying; Qi, Lili; Cho, Kenka; Zhou, Zhenhai; Li, Na; Hu, Yurong; Jiang, Baohong

doi:10.1016/j.phymed.2018.12.040

Cited by 25 publications

(14 citation statements)

References 25 publications

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“…Referring to the compatibility ratio of salvianolic acid B and ginsenoside Rg 1 , it was found that the content ratios of the two components were mostly between 2:3‐2:8 based on the quantitative results. Interestingly, when the compatibility ratio was set to 2:5, this components combination was reported to show superior effects on myocardial protection [19, 20]. This ratio also happened to be in the middle of the above range, thus we selected the ratio of 2:5 for combination to investigate the anti‐thrombotic activity of BRg 1 on the zebrafish model.…”

Section: Discussionmentioning

confidence: 99%

Quantitative evaluation of Danqi tablet by ultra‐performance liquid chromatography coupled with triple quadrupole mass spectrometry integrated with bioassay

Wang

Tao

et al. 2021

J of Separation Science

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Danqi tablet composed of the dried roots of Salvia miltiorrhiza and Panax notoginseng is a well‐known Chinese patent medicine commonly used for the treatment of cardio‐cerebrovascular diseases such as coronary heart disease and myocardial ischemia. Numerous chemical constituents belonging to S. miltiorrhiza and P. notoginseng were detectable in Danqi tablet. Here, we established and validated a rapid and sensitive ultra‐performance liquid chromatography coupled with triple quadrupole mass spectrometry method for simultaneous quantification of 23 components in Danqi tablet and then successfully applied to assay 12 batches of samples from ten manufacturers. Our results demonstrated that the contents of 23 components in 12 batches of Danqi tablets varied significantly and their quality indeed existed differently based on the principal component analysis. According to the quantitative data and the loading plot of principal component analysis, five abundant compounds in Danqi tablet were selected as characteristic chemical markers possibly responsible for the quality assessment. Among them, salvianolic acid B and ginsenoside Rg1 were further chosen to be combined at 2:5 ratio to evaluate the anti‐thrombotic activity on phenylhydrazine‐induced zebrafish heart thrombosis model. Expectedly, this component combination increased the heart red blood cells intensity compared with the model group and the median effective concentration was 123.4 µg/mL, suggestive of its well anti‑thrombotic effect. This study contributed to the quantitative evaluation of Danqi tablet and indicated the combination of salvianolic acid B and ginsenoside Rg1 may be capable of reflecting the effect of Danqi tablet, thereby providing a reference for further investigations on the improvement of quality control and clinical application of Danqi tablet.

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Section: Discussionmentioning

confidence: 99%

Quantitative evaluation of Danqi tablet by ultra‐performance liquid chromatography coupled with triple quadrupole mass spectrometry integrated with bioassay

Wang

Tao

et al. 2021

J of Separation Science

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“…Myocardial injury is accompanied by an increase in α-SMA and H-FABP. 7,8 At the same time, the synthesis and secretion of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) are important indicators of myocardial injury. 9 The levels of α-SMA and H-FABP in the serum, ANP and BNP in heart of the SHR group were significantly higher than the WKY group (P<0.01).…”

Section: Effect Of Geniposide On Myocardial Injury Markers In Ratsmentioning

confidence: 99%

Geniposide from Gardenia jasminoides var. radicans Makino Attenuates Myocardial Injury in Spontaneously Hypertensive Rats via Regulating Apoptotic and Energy Metabolism Signalling Pathway

Hou

Yuan

et al. 2021

DDDT

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Introduction Hypertension is closely related to myocardial injury. Long-term hypertension can cause myocardial injury. Therefore, it is very important to find drugs to treat myocardial injury caused by hypertension. The aim of present study is to investigate the effects and mechanisms of geniposide on myocardial injuries in spontaneously hypertensive rats (SHR) and H9c2 cells induced by NaCl solution. Materials and Methods Male Wistar–Kyoto (WKY) and SHR rats were given different doses of geniposide (25 mg/kg/d or 50 mg/kg/d) or distilled water for three consecutive weeks. Meanwhile, an H9c2 cell line-injury model was established using a solution of 150 µmol/L NaCl for 8 h. The cardiac function and related indexes of rats were detected. Results The results showed that geniposide decreased the levels of COI and COIII, which promoted the phosphorylation of AMPK (p-AMPK) and enhanced the energy metabolism pathway. Geniposide improved myocardial apoptosis by regulating apoptotic proteins (p38, BAX and Bcl-2). Finally, heart function was regulated, and the markers of myocardial injury were decreased. Geniposide increased the viability of H9c2 cells treated with the NaCl solution and decreased the rate of apoptosis by regulating the levels of apoptotic proteins. Geniposide could activate energy metabolism signalling pathway (AMPK/SirT1/FOXO1) and reduce H9c2 cell apoptosis. Conclusion Our results showed that the mechanisms by which geniposide improves myocardial injury in SHR may be through regulating the energy metabolism signalling pathway (AMPK/SirT1/FOXO1) and improving myocardial apoptosis by regulating apoptotic proteins.

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“…Shenfu injection (SFI), a known traditional Chinese herbs extract, includes ginsenoside and aconitine. Studies have proved that ginsenoside significantly protects against heart failure and improves cardiac function [ 14 – 16 ]. Aconitine is extracted from Fuzi, and modern pharmacological studies have proved that Fuzi exerts a protective effect on myocardial cells and could activate α and β -adrenergic receptors [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Shenfu Injection on Differentiation of Bone Marrow Mesenchymal Stem Cells into Pacemaker-Like Cells and Improvement of Pacing Function of Sinoatrial Node

Chen

Kang

et al. 2022

Oxidative Medicine and Cellular Longevity

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Sick sinus syndrome (SSS), a complex type of cardiac arrhythmia, is a major health threat to humans. Shenfu injection (SFI), a formula of traditional Chinese medicine (TCM), is effective in improving bradyarrhythmia. However, the underlying mechanism of SFI’s therapeutic effect is subject to few systematic investigations. The purpose of the present research is to examine whether SFI can boost the differentiation effectiveness of bone marrow mesenchymal stem cells (BMSCs) into pacemaker-like cells and whether the transplantation of these cells can improve the pacing function of the sinoatrial node (SAN) in a rabbit model of SSS. BMSCs from New Zealand rabbits were extracted, followed by incubation in vitro. The flow cytometry was utilized to identify the expression of CD29, CD44, CD90, and CD105 surface markers. The isolated BMSCs were treated with SFI, and the whole-cell patch-clamp method was performed to detect hyperpolarization-the activated cyclic nucleotide-gated potassium channel 4 (HCN4) channel current activation curve. The SSS rabbit model was established using the formaldehyde wet dressing method, and BMSCs treated with SFI were transplanted into the SAN of the SSS rabbit model. We detected changes in the body-surface electrocardiogram and recorded dynamic heart rate measurements. Furthermore, transplanted SFI-treated BMSCs were subjected to HE staining, TUNEL staining, qPCR, western blotting, immunofluorescence, immunohistochemistry, and enzyme-linked immunosorbent assay to study their characteristics. Our results indicate that the transplantation of SFI-treated BMSCs into the SAN of SSS rabbits improved the pacing function of the SAN. In vitro data showed that SFI induced the proliferation of BMSCs, promoted their differentiation capacity into pacemaker-like cells, and increased the HCN4 expression in BMSCs. In vivo, the transplantation of SFI treated-BMSCs preserved the function of SAN in SSS rabbits, improved the expression of the HCN4 gene and gap junction proteins (Cx43 and Cx45), and significantly upregulated the expression of cAMP in the SAN, compared to the SSS model group. In summary, the present research demonstrated that SFI might enhance the differentiation capacity of BMSCs into pacemaker-like cells, hence offering a novel approach for the development of biological pacemakers. Additionally, we confirmed the effectiveness and safety of pacemaker-like cells differentiated from BMSCs in improving the pacing function of the SAN.

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Cardioprotection of salvianolic acid B and ginsenoside Rg1 combination on subacute myocardial infarction and the underlying mechanism

Cited by 25 publications

References 25 publications

Quantitative evaluation of Danqi tablet by ultra‐performance liquid chromatography coupled with triple quadrupole mass spectrometry integrated with bioassay

Quantitative evaluation of Danqi tablet by ultra‐performance liquid chromatography coupled with triple quadrupole mass spectrometry integrated with bioassay

Geniposide from Gardenia jasminoides var. radicans Makino Attenuates Myocardial Injury in Spontaneously Hypertensive Rats via Regulating Apoptotic and Energy Metabolism Signalling Pathway

Effect of Shenfu Injection on Differentiation of Bone Marrow Mesenchymal Stem Cells into Pacemaker-Like Cells and Improvement of Pacing Function of Sinoatrial Node

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