Cardioprotection via Metabolism for Rat Heart Preservation Using the High-Pressure Gaseous Mixture of Carbon Monoxide and Oxygen

Suzuki, Chise; Hatayama, Naoyuki; Ogawa, Tadashi; Nanizawa, Eri; Otsuka, Shun; Hata, Koichiro; Seno, Hiroshi; Naito, Makoto; Hirai, Shuichi

doi:10.3390/ijms21228858

Cited by 9 publications

(4 citation statements)

References 25 publications

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“…Speculatively, Oroxylin A suppresses the R5P concentration by possibly inhibiting the pentose phosphate pathway and, consequently, purine metabolism, by decreasing the supply of PRPP, which is required for ATP generation and purine nucleotide biosynthesis in F. graminearum ( Figure 5 B) [ 28 , 29 ]. Alternatively, a downregulated pentose phosphate pathway may result in decreased NADPH generation and increased oxidative stress, which imbalances the redox system in F. graminearum , thereby retarding its growth and development [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Activity of Oroxylin A against Fusarium graminearum and Its Inhibitory Effect on Zearalenone Production

Zhou,

Hou,

Zhou

et al. 2023

Toxins

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Fusarium graminearum produces zearalenone (ZEA), a mycotoxin that is widely found in food and feed products and is toxic to humans and livestock. Piper sarmentosum extract (PSE) inhibits F. graminearum, and Oroxylin A appears to be a major antifungal compound in PSE. The aim of this study is to quantify the Oroxylin A content in PSE using UPLC-QTOF-MS/MS, and to investigate the antagonistic activity of Oroxylin A against F. graminearum and its inhibitory effect on ZEA production. The results indicate that Oroxylin A inhibits both fungal growth and ZEA production in a dose-dependent manner. Oroxylin A treatment downregulated the mRNA expression of zearalenone biosynthesis protein 1 (ZEB1) and zearalenone biosynthesis protein 2 (ZEB2). The metabolomics analysis of F. graminearum mycelia indicated that the level of ribose 5-phosphate (R5P) deceased (p < 0.05) after Oroxylin A treatment (64–128 ng/mL). Moreover, as the Oroxylin A treatment content increased from 64 to 128 ng/mL, the levels of cis-aconitate (p < 0.05) and fumarate (p < 0.01) were upregulated successively. A correlation analysis further showed that the decreased R5P level was positively correlated with ZEB1 and ZEB2 expression, while the increased cis-aconitate and fumarate levels were negatively correlated with ZEB1 and ZEB2 expression. These findings demonstrate the potential of Oroxylin A as a natural agent to control toxigenic fungi and their mycotoxin.

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Section: Discussionmentioning

confidence: 99%

Antagonistic Activity of Oroxylin A against Fusarium graminearum and Its Inhibitory Effect on Zearalenone Production

Zhou,

Hou,

Zhou

et al. 2023

Toxins

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“…The reason for this may be that excess MitoQ can stimulate the release of one-electron oxidizing species such as O 2 •− . There have been reports of excessive levels of oxidative stress induced by O 2 •− [ 34 ]. In addition, the O 2 •− anion radical is a key determinant of the overall effects of ROS [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Does Antioxidant Mitoquinone (MitoQ) Ameliorate Oxidative Stress in Frozen–Thawed Rooster Sperm?

Sun

et al. 2022

Animals

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In this study, we aimed to determine the benefit of mitoquinone (MitoQ) in rooster semen extenders on sperm quality, motility parameters, antioxidant capacities, and apoptotic changes in post-thawed rooster semen. A total of 85 ejaculates from 18 roosters were collected and then divided into five equal aliquots and cryopreserved in extenders with 1.0% soy lecithin nanoparticles that contained various concentrations of MitoQ (0 nM (M0), 50 nM (M50), 100 nM (M100), 150 nM (M150), and 200 nM (M200)). By using a computer-assisted semen analyzer, sperm motility parameters were assessed after freeze thawing. The M150 group had significantly higher percentages of total motility, progressive motility, viability, acrosome membrane integrity, and mitochondrial activity than the other groups (p < 0.05). Compared to other groups, M100 and M150 groups produced a higher percentage of plasma membrane integrity and ATP contents (p < 0.05). Additionally, the lowest levels of ROS and MDA in spermatozoa were observed in M150 group (p < 0.05), whereas the highest levels of ROS and MDA were observed in sperm in the controls or the M200 group (p < 0.05). Significantly higher values of SOD, GPx, and Cas-3 were found in the M150 group compared to other groups (p < 0.05). Overall, these results demonstrate that MitoQ at 150 nM not only ameliorates post-thawed sperm quality and motility parameters by restoring ATP levels and preventing membrane damage, but also improves redox balance and antiapoptotic activities.

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“…The mixture was found to reduce oxidative stress, resulting in cardiac protection by enhancing aerobic metabolism in vivo. Notably, the study revealed a significant increase in CA levels in the HPG group [5]. CA as a supplement in animal feed has a rich history dating back to 1937 when Pileggi et al discovered its positive effects on rat health, improving phosphorus utilization and preventing achondroplasia [6].…”

Section: Introductionmentioning

confidence: 90%

Citric Acid Promotes Immune Function by Modulating the Intestinal Barrier

Hu,

Yuan,

Guo

et al. 2024

IJMS

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Amidst increasing concern about antibiotic resistance resulting from the overuse of antibiotics, there is a growing interest in exploring alternative agents. One such agent is citric acid, an organic compound commonly used for various applications. Our research findings indicate that the inclusion of citric acid can have several beneficial effects on the tight junctions found in the mouse intestine. Firstly, the study suggests that citric acid may contribute to weight gain by stimulating the growth of intestinal epithelial cells (IE-6). Citric acid enhances the small intestinal villus–crypt ratio in mice, thereby promoting intestinal structural morphology. Additionally, citric acid has been found to increase the population of beneficial intestinal microorganisms, including Bifidobacterium and Lactobacillus. It also promotes the expression of important protein genes such as occludin, ZO-1, and claudin-1, which play crucial roles in maintaining the integrity of the tight junction barrier in the intestines. Furthermore, in infected IEC-6 cells with H9N2 avian influenza virus, citric acid augmented the expression of genes closely associated with the influenza virus infection. Moreover, it reduces the inflammatory response caused by the viral infection and thwarted influenza virus replication. These findings suggest that citric acid fortifies the intestinal tight junction barrier, inhibits the replication of influenza viruses targeting the intestinal tract, and boosts intestinal immune function.

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Cardioprotection via Metabolism for Rat Heart Preservation Using the High-Pressure Gaseous Mixture of Carbon Monoxide and Oxygen

Cited by 9 publications

References 25 publications

Antagonistic Activity of Oroxylin A against Fusarium graminearum and Its Inhibitory Effect on Zearalenone Production

Antagonistic Activity of Oroxylin A against Fusarium graminearum and Its Inhibitory Effect on Zearalenone Production

Does Antioxidant Mitoquinone (MitoQ) Ameliorate Oxidative Stress in Frozen–Thawed Rooster Sperm?

Citric Acid Promotes Immune Function by Modulating the Intestinal Barrier

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