Cardioprotective and antioxidant effects of taxifolin and vitamin C against diazinone-induced myocardial injury in rats

Abstract

In Iraq, excessive exposure to insecticides is increasingly becoming uncontrollable, the objective of this work was to assess the possible ameliorative role of taxifolin and vitamin c against diazinon-induced myocardial injury in rats. 36 sprague female rats divided into 6 groups of 6 rats each. Group 1 received diazinone 20 mg/kg gavaged for 30 days. Group 2 received taxifolin (25 mg/kg) and vitamin C (100 mg /kg) co-administered together and gavaged daily for 30 days before oral diazinone (20 mg/kg). Group 3 and group 4 were treated with taxifoline (25 mg/kg) and vitamin C (100 mg /kg) respectively, gavaged separately for 30 days before diazinone (20 mg/kg) administration. group 5 was considered as control 1 received taxifolin (25 mg/kg) and vitamin C (100 mg /kg) co-administered together and gavaged daily for 30 days. The last group, group 6 considered as control 2 which received vehicle of diazinon. Rats’ weights, water and food intake were measured and the general vital signs of rats were observed and noted. At the end of study, rats are sacrificed, blood withdrawn for biochemical analysis and heart were excised for glutathione peroxidase and pathological evaluation. Significant increase in body weights in all treated and control groups except group 1. Troponin, LDH, AST and ALT levels were significantly increased in group 1 revealing the existence of ischemia and damage in hearts. Treatment with taxifolin and vitamin C combination reduced their levels significantly to concentrations comparable to that in the control groups. Such finding supported by histopathological observation. Moreover, this study showed that diazinon administration significantly deteriorated lipid profile, co-administration of taxifolin and vitamin C was resulted in a significant improvement in lipid profile associated with increased in glutathione peroxidase values showing significant improvement in antioxidant levels. Sub-acute administration of pharmacological doses of taxifolin and vitamin C exerts cardioprotective effects against diazinon-induced myocardial injury in rats.

Cardioprotective Effect of Taxifolin against Isoproterenol-Induced Cardiac Injury through Decreasing Oxidative Stress, Inflammation, and Cell Death, and Activating Nrf2/HO-1 in Mice

“…This was evidenced by considerably lower MDA, protein carbonyl, and NO levels, while elevating SOD, CAT, and GSH in cardiac tissues of mice pre-treated with TAX prior to ISO administration. In accordance with our results, several investigations have shown that the cardioprotective action of TAX is associated with reduced oxidative markers and elevated antioxidant components, resulting in ameliorated oxidative injury to the myocardium [ 39 , 40 , 49 ]. Furthermore, TAX exhibited tissue protective effect against chemically-induced oxidative damage in various experimental models, such as acrylamide-induced heart [ 21 ] and kidney injury [ 50 ], alcohol [ 25 ] and carbon tetrachloride (CCl 4 )-induced liver injury [ 25 ], and methanol-induced oxidative optic nerve damage [ 51 ].…”

Effects of taxifolin from enzymatic hydrolysis of Rhododendron mucrotulatum on hair growth promotion

“…In addition, it has been proven to be effective in diabetic cardiomyopathy by inhibiting oxidative stress to regulate mitochondrial function and inhibit cell apoptosis ( Sun et al, 2014 ; Zheng et al, 2021 ). Moreover, taxifolin exerts cardioprotective effects against diazinon-induced myocardial injury in rats ( Najeb et al, 2022 ). However, it has been reported that taxifolin aglycone is found in complex preparations such as silymarin (LegalonTM), is presented in very small amounts, and is rarely used alone ( Kroll et al, 2007 ; Weidmann, 2012 ).…”

Enhancing the Bioavailability of Quercetin by Concomitant Administration with Enzyme Inhibitor

“…(8,12) It is one of more than 4000 plant phenolics that are naturally available, (13) in many vegetables, fruits and drinks ingested from the daily diet, (14) including onions, broccoli, apples, berries, (15) and citrus fruits, (9) it is ubiquitously present in wine and tea. (16,17) Que is well known for its vasodilator, antihypertensive effects, antiatherosclerotic, antiobesity, antihypercholesterolemic, and antidiabetic activities. (18)(19)(20) Que's mechanisms of action are pleiotropic, involves the decrease of glucose absorption from the intestine, insulin secretory and insulin-sensitizing effects, and the enhancement of glucose uptake in the muscles.…”