Cardioprotective effect of Phyllanthus amarus against high fructose diet induced myocardial and aortic stress in rat model

Putakala, Mallaiah; Gujjala, Sudhakara; Nukala, Srinivasulu; Bongu, Sasi Bhusana Rao; Chintakunta, Nagaraju; Desireddy, Saralakumari

doi:10.1016/j.biopha.2017.09.054

Cited by 13 publications

(16 citation statements)

References 53 publications

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“…This was confirmed in this study by the significant increase of MDA, a sensitive biomarker of lipid peroxidation with significant decrease of the antioxidant enzyme SOD in testicular homogenates of HFrD rats, clearly indicating oxidative stress and impaired lipid metabolism. Similar results were recorded in the liver [72], the kidney [81], the heart and aorta [61] of HFrD fed rats. This was attributed to altered cellular metabolism with overproduction of ROS and simultaneous decline of antioxidant defense mechanisms as explained by Chandramohan and Pari [21].…”

Section: Discussionsupporting

confidence: 85%

Effect of high-fructose diet-induced metabolic syndrome on the pituitary-gonadal axis from adolescence through adulthood in male albino rats and the possible protective role of ginger extract. A biochemical, histological and immunohistochemical study

El-Mehi

Faried

2020

Folia Morphol

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Background: This work was designed to clarify, for the first time up to our knowledge, the effects of high fructose diet (HFrD) induced metabolic syndrome (MetS) on the pituitarygonadal axis of adolescent male rats continuing through adulthood period and to evaluate the possible role of ginger extract in ameliorating these effects. Materials and methods:Forty 4-week-old male albino rats, treated for 8 weeks, were randomly divided into 4 equal groups; control(fed standard diet), ginger extract treated (500 mg/kg once daily orally by gavage), HFrD induced MetS (fed a diet containing 60% fructose), HFrD and ginger groups. The assessment methods included biochemical, histological and immunohistochemical studies.Results: HFrD fed rats exhibited a picture similar to MetS in the form of increased body weight and serum levels of glucose and insulin with an elevated HOMA-IR reflecting insulin resistance as well as dyslipidemia. Lipid peroxidation (increased MDA) and oxidative stress (decreased SOD) were implicated in this syndrome. This group exhibited a significant decrease of testicular weight and the levels of reproductive hormones (LH, FSH & testosterone). The pituitary gonadotrophs showed electron dense nuclei, large cytoplasmic vacuoles, destructed organelles in addition to decreased number of secretory granules. Furthermore, testicular specimens presented marked alterations. There were disorganized shrunken tubules with irregular basement membranes, reduced germinal epithelial thickness, vacuolations and degenerated mitochondria in the spermatogenic cells.Beclin1 and PCNA immunoexpressions were significantly downregulated with HFrD induced MetS. Ginger extract supplementation proved to be a potent protective agent against these harmful effects through its antioxidant, hypoglycemic, insulinotropic, androgenic and hypolipidemic effects in addition to its ability to induce testicular autophagy. Conclusions: Consumption of HFrD induced MetS and proved to have harmful effects on the pituitarytesticular axis. Moreover, this work provided a new insight into the possible use of ginger extract to alleviate the main features of MetS and protect the pituitarygonadal axis from the damaging effects of HFrD induced MetS.

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Section: Discussionsupporting

confidence: 85%

Effect of high-fructose diet-induced metabolic syndrome on the pituitary-gonadal axis from adolescence through adulthood in male albino rats and the possible protective role of ginger extract. A biochemical, histological and immunohistochemical study

El-Mehi

Faried

2020

Folia Morphol

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“…In addition to effects of QCT supplementation in pure form, 60-days treatment with Phyllanthus amarus (plant reach in QCT) showed protection of the heart from high fructose-diet induced damage. The Phyllanthus amarus treatment protected male Wistar rats from high fructose-diet-induced increase in stress markers and a decrease in non-enzymatic and enzymatic antioxidants in the heart and aorta [90]. An interesting form of QCT administration was used in study Cote et al [77], where a combination of QCT with Resveratrol in Pluronic ® F-127 micelles (mRQ) (RES:QCTin 1:1 molar ratio, capable of retaining 1.1 mg/mL of Resveratrol and 1.42 mg/mL of QCT, respectively) was prepared for application.…”

Section: In Vivo Cardioprotection Afforded By Qct and Qct-rich Plantsmentioning

confidence: 99%

Potential Implications of Quercetin and its Derivatives in Cardioprotection

Ferenczyová

Kaločayová

Barteková

2020

IJMS

106

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Quercetin (QCT) is a natural polyphenolic compound enriched in human food, mainly in vegetables, fruits and berries. QCT and its main derivatives, such as rhamnetin, rutin, hyperoside, etc., have been documented to possess many beneficial effects in the human body including their positive effects in the cardiovascular system. However, clinical implications of QCT and its derivatives are still rare. In the current paper we provide a complex picture of the most recent knowledge on the effects of QCT and its derivatives in different types of cardiac injury, mainly in ischemia-reperfusion (I/R) injury of the heart, but also in other pathologies such as anthracycline-induced cardiotoxicity or oxidative stress-induced cardiac injury, documented in in vitro and ex vivo, as well as in in vivo experimental models of cardiac injury. Moreover, we focus on cardiac effects of QCT in presence of metabolic comorbidities in addition to cardiovascular disease (CVD). Finally, we provide a short summary of clinical studies focused on cardiac effects of QCT. In general, it seems that QCT and its metabolites exert strong cardioprotective effects in a wide range of experimental models of cardiac injury, likely via their antioxidant, anti-inflammatory and molecular pathways-modulating properties; however, ageing and presence of lifestyle-related comorbidities may confound their beneficial effects in heart disease. On the other hand, due to very limited number of clinical trials focused on cardiac effects of QCT and its derivatives, clinical data are inconclusive. Thus, additional well-designed human studies including a high enough number of patients testing different concentrations of QCT are needed to reveal real therapeutic potential of QCT in CVD. Finally, several negative or controversial effects of QCT in the heart have been reported, and this should be also taken into consideration in QCT-based approaches aimed to treat CVD in humans.

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“…To scientifically support the traditional use of P. amarus, several pharmacological studies were conducted with extracts from the leaves and compounds isolated thereof. Antiallodynic [10], antiinflammatory [11], analgesic [12], cardioprotective [13], hepatoprotective [14], antioxidant [15], cytotoxic [16,17], immunosuppressive [18,19], antiparasitic [20,21], antiviral [22,23], antibacterial [24,25] antidiabetic [26], antihypertensive [27], chondroprotective [28], osteogenic [29], and diuretic properties [30] have been reported for the constituents of P. amarus.…”

Section: Prospecting and Identifying Phyllanthus Amarus Lignans With mentioning

confidence: 99%

“…Subfraction A (260 mg) which contained compounds 3 and 4 as revealed by GC-MS analysis was further purified by flash chromatography [silica gel, 50 g, 23 cm height × 3.5 cm diameter, RediSep Rf, hexane-EtOAc (90 : 10)] to provide a sample (127 mg) composed of only cubebin dimethyl ether 3 (85 mg), and 10 (174 mg) was performed as previously described [10]. These compounds were identified through GC-MS, 1 HNMR, and 13 CNMR analysis.…”

Section: Extraction and Isolationmentioning

confidence: 99%

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Prospecting and Identifying Phyllanthus amarus Lignans with Antileishmanial and Antitrypanosomal Activity

et al. 2020

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Ten lignans (1 – 10) were isolated from the hexane-ethyl acetate extract of Phyllanthus amarus leaves. Three of them, cubebin dimethyl ether (3), urinatetralin (4), and lintetralin (7) are described for the first time in this species, while phyllanthin (1), niranthin (2), 5-demethoxyniranthin (5), isolintetralin (6), hypophyllanthin (8), nirtetralin (9), and phyltetralin (10) have been already reported from P. amarus. Among the lignans tested against Trypanosoma cruzi intracellular amastigotes, 2 was the most active with an EC50 of 35.28 µM. Lignans 2, 5, 7, and 9 showed inhibitory effects against Leishmania amazonensis promastigotes with EC50 of 56.34, 51.86, 23.57, and 43.27 µM, respectively. During in vitro infection assays, 5 reduced amastigotes by 91% at 103.68 µM concentration, whereas 7 and 9 reduced amastigotes by approximately 84% at 47.5 and 86.04 µM, respectively. Lignans 5, 7, and 9 were more potent in intracellular amastigotes with EC50 of 2.76, 8.30, and 15.83 µM, respectively, than in promastigotes. CC50 for all samples was > 100 µg/mL, thus revealing low cytotoxicity against macrophages, and selectivity against the parasite. L. amazonensis promastigotes treated with compounds 2 and 9 showed decreased respiratory control of 38% and 25%, respectively, suggesting a change in mitochondrial membrane potential and lower ATP production.

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Cardioprotective effect of Phyllanthus amarus against high fructose diet induced myocardial and aortic stress in rat model

Cited by 13 publications

References 53 publications

Effect of high-fructose diet-induced metabolic syndrome on the pituitary-gonadal axis from adolescence through adulthood in male albino rats and the possible protective role of ginger extract. A biochemical, histological and immunohistochemical study

Effect of high-fructose diet-induced metabolic syndrome on the pituitary-gonadal axis from adolescence through adulthood in male albino rats and the possible protective role of ginger extract. A biochemical, histological and immunohistochemical study

Potential Implications of Quercetin and its Derivatives in Cardioprotection

Prospecting and Identifying Phyllanthus amarus Lignans with Antileishmanial and Antitrypanosomal Activity

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