Cardioprotective effect of ultrasound‐targeted destruction of Sirt3‐loaded cationic microbubbles in a large animal model of pathological cardiac hypertrophy

Ultrasound-targeted microbubble technology facilitates SAHH gene delivery to treat diabetic cardiomyopathy by activating AMPK pathway

“… 6 , 22 Indeed, translational research using large animals revealed that UTMD has great prospects for application in gene therapy as indicated by the results in porcine myocardial hypertrophy models. 12 Several genes have been shown to improve cardiac function and cardiomyopathy in diabetic rats, with SAHH recently receiving increased attention.…”

“…We showed that CMBs improved UTMD-mediated gene transfection; however, particular modifications are still required, including the development of nanocomplex-conjugated microbubbles, which will further enhance the stability or delivery efficiency and increase transgene expression in the target area. 29 UTMD-mediated gene therapy is a promising approach in the field of medicine, with potential utility not only in cardiology and cardiovascular therapies 12 , 15 but also in the treatment of tumors. 30 …”

“… 10 Ultrasound-targeted microbubble destruction (UTMD) is a novel gene-targeting delivery system that provides minimally invasive, repeatable, and targeted gene delivery to injured myocardial tissue, improving cardiac function. 11 , 12 Clinical trials have shown that ultrasound microbubbles are safe for thrombolytic therapy and tumor adjuvant chemotherapy. 13 , 14 In our previous study, we developed a positively charged carrier called cationic microbubbles (CMBs), the binding ability of which was enhanced by the incorporation of the melatonin receptor RORα.…”

Cardiac gene delivery using ultrasound: State of the field

“… Cardiomyopathy (CM) Qin et al. 2023 92 Sirt3/Porcine/#5/ear vein (i.v.) US system: EPIQ 5 (Philips) Probe/Frequency: S5-1 Pressure/Power: MI = 1.2–1.35 Pulse length: single frame Interval: triggered every 4 cardiac cycles Total duration: 30 min.…”

SIRT3 regulates cardiolipin biosynthesis in pressure overload-induced cardiac remodeling by PPARγ-mediated mechanism

“…Silent mating type information regulation 2 homolog 3 (sirtuin 3, or SIRT3), a sirtuin family member mainly located in mitochondria, has been reported to have protective effects in various HF phenotypes [ 11 , 12 ]. In our previous studies, we found that mitochondrial lipid metabolism disorders occurred in the hypertrophic myocardium of SIRT3 knockout (SIRT3 −/− ) mice.…”