Cardioprotective effects of Achillea wilhelmsii on the isolated rat heart in ischemia–reperfusion

Abstract

ContextThere are some reports about protective effects of Achillea on the heart.ObjectiveWe investigated the effect of Achillea wilhelmsii extract on cardiac function during ischemia–reperfusion (I/R) injury in the isolated rat heart.Materials and methods60 male Wistar rats were randomly divided into 6 groups; 1: Control group, 2: Control-ischemia (CI) 3: vitamin C (10 mg/kg), 4–6: Extract groups (E 100, E 200 and E 400 mg/kg). The animals received normal saline, vitamin C or A. wilhelmsii extract orally for 4 weeks. At the end of the treatment, the hearts were subjected to in vitro I/R Injury (20 min of global ischemia, followed by 40 min of reperfusion, Langendorff's mode). Heart rate (HR) and left ventricular pressure (LVP) were measured using a pressure transducer connected to a data acquisition system. Lactate dehydrogenase (LDH) and creatine kinase (CK) activities in the effluent were measured to determine the myocardial injury degree. The malondialdehyde (MDA), total thiol groups (-SH), superoxide anion dismutase (SOD) and catalase (CAT) in myocardial tissue were detected to determine the oxidative stress degree.ResultsPretreatment with Achillea wilhlemsii significantly decreased the LDH, CK activities, and MDA level, while it increased the LVDP, ±dp/dtmax, rate-pressure product (RPP), SH groups, SOD and CAT activities, and also the coronary artery flow.Discussion and conclusionOur findings indicated that Achillea wilhelmsii could provide protection for heart against the I/R injury which may be related to the improvement of myocardial oxidative stress states.

A Histopathological Study of Cisplatin-induced Acute Vascular Injuries in Vital Organs and Protective Effect of Achillea millefolium

“…Due to the effect of "Achillea millefolium" extract on increasing levels of antioxidant enzymes in the heart muscle, Mahmoudabadi et al have reported a positive effect of this extract on antioxidant enzymes level in rat model of heart ischemia-reperfusion [69] The results of our experiments also have showed the beneficial effect of this plant against the side effects of cisplatin.…”

Achillea spp.: A comprehensive review on its ethnobotany, phytochemistry, phytopharmacology and industrial applications

“…A. wilhelmsii is able to decrease the activity of lactate dehydrogenase (LDH), creatine kinase (CK) and malondialdehyde (MDA) levels, and to increase the superoxide dismutase (SOD) and catalase (CAT) activities. In summary, it was able to improve the myocardial oxidative stress states on cardiac function during ischemia/reperfusion injury in rat (15). A. phrygia ethyl acetate extracts also showed a remarkable enzyme inhibitory potential (16), and the hydroalcoholic extract of A. millefolium demonstrated in vivo antidiabetic effects, through inhibition of α-glucosidases, insulin secretion and insulin secretagogue activities (17).…”

Salvigenin, a Trimethoxylated Flavone from Achillea Wilhelmsii C. Koch, Exerts Combined Lipid-Lowering and Mitochondrial Stimulatory Effects

“…This plant holds a remarkable place in traditional Persian medicine, with a particular indication for the treatment of pulmonary affections, and it is used by the local population for a variety of ailments [4]. Recent investigations have substantiated these traditional uses with a scientific approach and activity on irritable bowel syndrome and ulcerative colitis [5,6], and improvement of oxidative stress-related myocardial injury [7] have been demonstrated. Asgary et al [8] have reported a significant activity of A. wilhelmsii extracts in inducing a decrease in triglycerides and LDL-cholesterol levels after 2-4 months of the administration, a result supported by Khazneh et al [9] in a more recent investigation.…”