Cardioprotective Effects of HO-1-Loaded Collagen-Targeted Phase Change Nanoparticles on Cardiomyocytes Following Acute Myocardial Infarction

Abstract

Acute myocardial infarction (MI) is a major cause of death worldwide. This study utilized collagen-targeted phase change material (PCM) nanoparticles (NPs) to co-encapsulate HO-1 and explored the efficacy of composite PCM NPs on cardiomyocyte progression and development of MI. In this study, we enrolled 32 acute MI patients and 32 healthy participants, and ELISA assay was used to assess the content of creatine kinase isoenzyme (CK-MB). Mice with MI received tail vein administration of HO-1-loaded PCM NPs, followed by RT-qPCR detection of expressions of hypoxia-reoxygenation related genes (SpA, SpB, SpC, Occludin, KGF, and CK18). Patients with acute MI had a higher level of CK-MB. Treatment with HO-1-loaded collagen-targeted PCM NPs decreased expressions of SpA, SpB, SpC, Occludin, KGF, and CK18, facilitating repair of damaged tissues. Of note, NPs loaded with siRNA HO-1 up-regulated the expression of these genes. Collagen-targeted PCM NPs carrying HO-1 effectively promoted the repair of myocardial cells and relieved MI through down-regulation of hypoxia-reoxygenation related genes, which may enhance prevention and treatment for acute MI.

Farnesol Protects against Cardiotoxicity Caused by Doxorubicin-Induced Stress, Inflammation, and Cell Death: An In Vivo Study in Wistar Rats

“…The cardiotoxic role of persistent oxidative and nitrative stress is well-established in various preclinical and clinical studies. Several cardiotoxic agents exhibit cardiotoxicity via inducing oxidative and nitrative stress, whereas cardioprotective agents showed its effect via scavenging these free radicals [ 41 , 42 , 43 ]. Considering these facts, in the present study, DOXO administration caused a significant increase in lipid peroxidation, exhibited by distinct elevations of TBARS, along with an increase in the level of NO [ 42 ].…”

Polydopamine Surface Engineering of Iron Single-Atom Nanozyme: a Novel Strategy for Doxorubicin Immobilization, Tumor Microenvironment Remodeling and Synergistic Multimodel Therapy

“…Combing single atom nanozymes with drugs has shown promise in achieving synergistic therapy, as evidenced by the cobalt-single-atom nanozyme when combined with doxorubicin (DOX) 24 . However, the e cacy of current anti-tumor drugs is limited by their weak anti-tumor effect and poor accumulation at the tumor site, leading to potential damage to hematopoietic stem cells and renal toxicity 25 . Therefore, developing a strategy to chemically immobilize anticancer drugs on the surface of single-atom nanozymes presents a valuable opportunity.…”