Cardiotoxic effects of Loxosceles intermedia spider venom and the recombinant venom toxin rLiD1

Dias-Lopes, Camila; Felicori, Liza; Guimarães, Gabriela; Gomes, Enéas Ricardo de Morais; Roman‐Campos, Danilo; Duarte, Hugo L.; Damasceno, Dênis Derly; Martins, Marta; Kalapothakis, Evanguedes; Almeida, Alvair P.; Granier, Claude; Cruz, Jáder Santos; Guatimosim, Sílvia; Chávez-Olórtegui, Carlos

doi:10.1016/j.toxicon.2010.08.008

Cited by 27 publications

(26 citation statements)

References 39 publications

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“…This could explain the reduction in the peak of left ventricle systolic pressure observed in the present work. Also, mice envenomed with crude Loxoceles venom showed a reduction in the force production (Dias-Lopes et al, 2010). The reduction in the maximum speed of relaxation of the left ventricle (dP/dt min ) observed here suggest that rLiD1 can affect the mechanisms responsible for the reduction of intracellular calcium concentration during diastole (calcium uptake by the sarcoplasmic reticulum and/or the activity of sodium calcium exchange, calcium pump present in the sarcolema).…”

Section: Resultsmentioning

confidence: 63%

“…Clinical findings of the systemic effects are hemolytic anemia, thrombocytopenia resulting from platelet aggregation and intravascular coagulation, events that culminate in decreased hematocrit (Peterson, 2006). The ELISA method detected circulating antigens in kidney, heart, lung and liver of poisoned rats (Dias-Lopes et al, 2010). The main reason of death in cases of loxoscelism is due to acute renal failure (Futrell, 1992;Peterson, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Hearts of mice poisoned by phospholipase D have increased the calcium current and L-type channels also increase the calcium transient, although the force of systolic contraction is decreased (Dias-Lopes et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Recombinant Toxin Phospholipase D in Cardiac Muscle of Rats

Peixoto¹,

Dias²,

Damiani³

et al. 2015

American Journal of Pharmacology and Toxicology

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Loxoceles spiders gender are worldwide spread and its bites can cause dermonecrosis or even a systemic effect (hemolysis, kidney and liver injury). It is believed that phospholipase D, the main component present in the venom, could be responsible for the injury. In this study, we used a recombinant form of phospholipase D (rLiD1) and evaluated its direct and systemic effects on the contractility of papillary muscles and in the left intra ventricular pressure of isolated perfused hearts, respectively. In papillary muscle directly exposed to rLiD1 no effects on force, maximum speed of contraction (df/dt max ) or relaxation (df/dt min ) were observed. In isolated perfused heart, the peak of systolic pressure and the rate of relaxation (dP/dt min ) were reduced in animals treated with rLiD1. However, the maximum speed of pressure developed during contraction (dP/dt max ) was unaffected. These data suggest that rLiD1 did not affect directly the excitation contraction coupling or the contractility of the myocardium but its systemic effect can induce reduction in the cardiac performance.

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Section: Resultsmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Recombinant Toxin Phospholipase D in Cardiac Muscle of Rats

Peixoto¹,

Dias²,

Damiani³

et al. 2015

American Journal of Pharmacology and Toxicology

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“…Because this cascade can be activated by interactions outside the cell, we speculate that the venom of L. similis may interact with receptors on the cell surface. Many studies have shown the binding of Loxosceles venom toxins upon the membrane of diverse cell types, showing direct induction of cytotoxicity and supporting the hypothesis of “planted antigens” to different components of cell structures (Chaim et al, 2006; Chaves-Moreira et al, 2009; Dias-Lopes et al, 2010; Wille et al, 2013). Further research should be done to investigate which venom compounds can activate the caspases cascade investigated.…”

Section: Discussionmentioning

confidence: 78%

Whole venom of Loxosceles similis activates caspases-3, -6, -7, and -9 in human primary skin fibroblasts

Dantas

Horta

Martins

et al. 2014

Toxicon

Self Cite

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Spiders of the Loxosceles genus represent a risk to human health due to the systemic and necrotic effects of their bites. The main symptoms of these bites vary from dermonecrosis, observed in the majority of cases, to occasional systemic hemolysis and coagulopathy. Although the systemic effects are well characterized, the mechanisms of cell death triggered by the venom of these spiders are poorly characterized. In this study, we investigated the cell death mechanisms induced by the whole venom of the spider Loxosceles similis in human skin fibroblasts. Our results show that the venom initiates an apoptotic process and a caspase cascade involving the initiator caspase-9 and the effector caspases-3, -6, and -7.

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“…The thorax was opened, the heart was rapidly excised and immediately cooled in iced buffer and perfused through an aortic stump with Krebs-Ringer solution (KRS) containing: 118.4 mM NaCl, 4.7 mM KCl, 1.2 mM KH 2 PO 4 , 1.2 mM MgSO 4 ·7H 2 O, 2.5 mM CaCl 2 ·2H 2 O, 11.7 mM glucose, and 26.5 mM NaHCO 3 . The high glucose concentration was employed to overcome possible reductions in glucose uptake by cardiomyocytes (15). The perfusion fluid was maintained at 37 ± 1°C with constant pressure (75 mmHg) and oxygenation (5% CO 2 /95% O 2 ).…”

Section: Methodsmentioning

confidence: 99%

Anticonvulsant and antiarrhythmic effects of nifedipine in rats prone to audiogenic seizures

Damasceno

Ferreira

Doretto

et al. 2012

Braz J Med Biol Res

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Calcium ion participates in the regulation of neural transmission and the presynaptic release of neurotransmitters. It is also involved in epileptic events, cardiac arrhythmias and abnormal conduction of stimuli. The purpose of the present study was to evaluate the effects of nifedipine, a calcium channel blocker, on epileptic seizures and on reperfusion arrhythmias in rats prone to audiogenic epileptic seizures (Wistar audiogenic rats, WAR) and in normal Wistar rats (N = 6/group). The seizure severity index was applied after an intraperitoneal injection of 20 or 40 mg/kg nifedipine (N20 and N40 groups, respectively). The Langendorff technique was used to analyze cardiac function, as well as the incidence and severity of the reperfusion arrhythmias after ligature and release of the left coronary artery in rats treated or not with nifedipine. We found that nifedipine treatment decreased seizure severity (0.94 ± 0.02 for WAR; 0.70 ± 0.10 for WAR + N20; 0.47 ± 0.08 for WAR + N40) and increased the latent period (13 ± 2 s for WAR; 35 ± 10 s for WAR + N20; 48 ± 7 s for WAR + N40) for the development of seizures in WAR. Furthermore, the incidence and severity of the reperfusion arrhythmias were lower in WAR and normal Wistar rats injected with nifedipine. In WAR, these effects were mediated, at least in part, by a decrease in heart rate. Thus, our results indicate that nifedipine may be considered to be a potential adjuvant drug for epilepsy treatment, especially in those cases associated with cardiac rhythm abnormalities.

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Cardiotoxic effects of Loxosceles intermedia spider venom and the recombinant venom toxin rLiD1

Cited by 27 publications

References 39 publications

Effects of Recombinant Toxin Phospholipase D in Cardiac Muscle of Rats

Effects of Recombinant Toxin Phospholipase D in Cardiac Muscle of Rats

Whole venom of Loxosceles similis activates caspases-3, -6, -7, and -9 in human primary skin fibroblasts

Anticonvulsant and antiarrhythmic effects of nifedipine in rats prone to audiogenic seizures

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