Cardiotoxicity of allylamine and levels of serum cardiac troponin I in the Han Wistar rat

Brady, Sally; York, Malcolm; Guionaud, Silvia; Gayner, Carrie; Griffiths, William; Roman, Ian; Stamp, Clare; Williams, Thomas; Turton, John

doi:10.1016/j.tox.2006.05.076

Cited by 3 publications

(3 citation statements)

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“…Preliminary reports of these findings have been published in abstract form (Chen et al, 2004;Brady et al, 2005).…”

Section: Introductionmentioning

confidence: 97%

“…The purposes of the present investigations were: (1) to assess the linearity and precision of the cTnI assay using the ACS: 180SE (Bayer), together with the storage stability of the rat-specific immunoreactive cTnI signal; (2) to identify the onset of the release of cTn and correlate this timepoint with the nature and severity of the histopathologic lesion in the heart from 1 to 48 hours after the administration of ISO; (3) to identify the magnitude of the cTn signal with increasing dose levels of ISO over the dose range 0.25 to 20.0 mg/kg; (4) to correlate the magnitude and duration of the cTnI signal (ACS: 180SE; Bayer Healthcare Diagnostics) with the cTnT signal (Elecsys 2010; Roche Diagnostics) and also relate these 2 signals to the cTnI signal obtained with the DPC Immulite 2000 (Diagnostic Products Corporation); and, (5) to correlate the magnitude and duration of the cTnI signal with the more traditional biomarkers of cardiomyocyte injury, and in particular with both creatine kinase (CK) and lactate dehydrogenase (LD) isoenzymes. Preliminary reports of these findings have been published in abstract form (Chen et al, 2004;Brady et al, 2005).…”

Section: Introductionmentioning

confidence: 97%

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Characterization of Troponin Responses in Isoproterenol-Induced Cardiac Injury in the Hanover Wistar Rat

et al. 2007

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The investigations aimed to evaluate the usefulness of cardiac troponins as biomarkers of acute myocardial injury in the rat. Serum from female Hanover Wistar rats treated with a single intraperitoneal (IP) injection of isoproterenol (ISO) was assayed for cardiac troponin I (cTnI) (ACS: 180SE, Bayer), cTnI (Immulite 2000, Diagnostic Products Corporation) and cardiac troponin T (cTnT) (Elecsys 2010, Roche). In a time-course study (50.0 mg/kg ISO), serum cTnI (ACS:180SE) and cTnT increased above control levels at 1 hour postdosing, peaking at 2 hours (cTnI, 4.30 microg/L; cTnT, 1.79 microg/L), and declined to baseline by 48 hours, with histologic cardiac lesions first seen at 4 hours postdosing. The Immulite 2000 assay gave minimal cTnI signals, indicating poor immunoreactivity towards rat cTnI. In a dose-response study (0.25 to 20.0 mg/kg ISO), there was a trend for increasing cTnI (ACS:180SE) values with increasing ISO dose levels at 2 hours postdosing. By 24 hours, cTnI levels returned to baseline although chronic cardiac myodegeneration was present. We conclude that serum cTnI and cTnT levels are sensitive and specific biomarkers for detecting ISO induced myocardial injury in the rat. Serum troponin values reflect the development of histopathologic lesions; however peak troponin levels precede maximal lesion severity.

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“…Preliminary reports of these findings have been published in abstract form (Chen et al, 2004;Brady et al, 2005).…”

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Characterization of Troponin Responses in Isoproterenol-Induced Cardiac Injury in the Hanover Wistar Rat

et al. 2007

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“…Preliminary findings on these studies have been recorded in abstract form (Brady et al 2005) and in a more comprehensive report (Brady 2008).…”

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confidence: 99%

Cardiac Troponin I in Isoproterenol-Induced Cardiac Injury in the Hanover Wistar Rat: Studies on Low Dose Levels and Routes of Administration

et al. 2010

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The current studies demonstrate the effect of low-dose intraperitoneal (IP) administration of isoprotenerol (ISO) and subcutaneous (SC) versus IP routes of administration of ISO on serum cardiac troponin I (cTnI) levels in female Hanover Wistar rats, providing additional evidence to support acceptance of cTnI as a cardiac biomarker. At 2 hr postdosing with 0-500 microg/kg ISO, mean serum cTnI levels were increased in a dose-related fashion at > or =10 microg/kg with no evidence of cardiac pathology. At 24 h, cTnI concentrations were generally at control levels, but histologic cardiomyocyte injury was evident in a proportion of the animals given > or =10 microg/kg. In a second experiment, rats given SC ISO at 5,000 microg/kg and necropsied at 0, 1, 2, and 4 hr postdosing had higher levels of serum cTnI than animals given the same dose IP.

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Cardiac troponin is the most effective translational safety biomarker for myocardial injury in cardiotoxicity

2008

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Cardiotoxicity of allylamine and levels of serum cardiac troponin I in the Han Wistar rat

Cited by 3 publications

References 1 publication

Characterization of Troponin Responses in Isoproterenol-Induced Cardiac Injury in the Hanover Wistar Rat

Characterization of Troponin Responses in Isoproterenol-Induced Cardiac Injury in the Hanover Wistar Rat

Cardiac Troponin I in Isoproterenol-Induced Cardiac Injury in the Hanover Wistar Rat: Studies on Low Dose Levels and Routes of Administration

Cardiac troponin is the most effective translational safety biomarker for myocardial injury in cardiotoxicity

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