Cardiotoxicity of lead at various perfusate calcium concentrations: Functional and metabolic responses of the perfused rat heart

Prentice, Robert C.; Kopp, Stephen J.

doi:10.1016/0041-008x(85)90420-x

Cited by 43 publications

(34 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, Williams et al (16) reported negative inotropic effects on perfused hearts caused by acute lead administration. Prentice and Kopp (17) reported that lead significantly attenuated positive inotropic response to increasing calcium concentrations in perfused hearts. Bernal et al (18) reported a calcium channel blocker action of lead in rat myocytes and Xenopus laevis oocytes.…”

Section: Introductionmentioning

confidence: 99%

Lead reduces tension development and the myosin ATPase activity of the rat right ventricular myocardium

Vassallo

Lebarch

Moreira

et al. 2008

Braz J Med Biol Res

View full text Add to dashboard Cite

Lead (Pb 2+ ) poisoning causes hypertension, but little is known regarding its acute effects on cardiac contractility. To evaluate these effects, force was measured in right ventricular strips that were contracting isometrically in 45 male Wistar rats (250-300 g) before and after the addition of increasing concentrations of lead acetate (3,7,10,30, 70, 100, and 300 µM) to the bath. Changes in rate of stimulation (0.1-1.5 Hz), relative potentiation after pauses of 15, 30, and 60 s, effect of Ca 2+ concentration (0.62, 1.25, and 2.5 mM), and the effect of isoproterenol (20 ng/mL) were determined before and after the addition of 100 µM Pb 2+ . Effects on contractile proteins were evaluated after caffeine treatment using tetanic stimulation (10 Hz) and measuring the activity of the myosin ATPase. Pb 2+ produced concentration-dependent force reduction, significant at concentrations greater than 30 µM. The force developed in response to increasing rates of stimulation became smaller at 0.5 and 0.8 Hz. Relative potentiation increased after 100 µM Pb 2+ treatment. Extracellular Ca 2+ increment and isoproterenol administration increased force development but after 100 µM Pb 2+ treatment the force was significantly reduced suggesting an effect of the metal on the sarcolemmal Ca 2+ influx. Concentration of 100 µM Pb 2+ also reduced the peak and plateau force of tetanic contractions and reduced the activity of the myosin ATPase. Results showed that acute Pb 2+ administration, although not affecting the sarcoplasmic reticulum activity, produces a concentration-dependent negative inotropic effect and reduces myosin ATPase activity. Results suggest that acute lead administration reduced myocardial contractility by reducing sarcolemmal calcium influx and the myosin ATPase activity. These results also suggest that lead exposure is hazardous and has toxicological consequences affecting cardiac muscle.

show abstract

Section: Introductionmentioning

confidence: 99%

Lead reduces tension development and the myosin ATPase activity of the rat right ventricular myocardium

Vassallo

Lebarch

Moreira

et al. 2008

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…Additionally, chronic and acute lead poisoning causes cardiac and vascular damage with potentially lethal consequences (8,9). Lead also has other effects on the cardiovascular system, www.bjournal.com.br Braz J Med Biol Res 43 (5) 2010 including endothelial dysfunction (10) and inhibition of the sarcolemmal sodium potassium ATPase (11).…”

mentioning

confidence: 99%

Acute lead-induced vasoconstriction in the vascular beds of isolated perfused rat tails is endothelium-dependent

Silveira

Lizardo

Souza

et al. 2010

Braz J Med Biol Res

View full text Add to dashboard Cite

Chronic lead exposure induces hypertension in humans and animals, affecting endothelial function. However, studies concerning acute cardiovascular effects are lacking. We investigated the effects of acute administration of a high concentration of lead acetate (100 µΜ) on the pressor response to phenylephrine (PHE) in the tail vascular bed of male Wistar rats. Animals were anesthetized with sodium pentobarbital and heparinized. The tail artery was dissected and cannulated for drug infusion and mean perfusion pressure measurements. Endothelium and vascular smooth muscle relaxation were tested with acetylcholine (5 µg/100 µL) and sodium nitroprusside (0.1 µg/100 µL), respectively, in arteries precontracted with 0.1 µM PHE. Concentrationresponse curves to PHE (0.001-300 µg/100 µL) were constructed before and after perfusion for 1 h with 100 µΜ lead acetate. In the presence of endothelium (E + ), lead acetate increased maximal response (E max ) (control: 364.4 ± 36, Pb 2+ : 480.0 ± 27 mmHg; P < 0.05) and the sensitivity (pD 2 ; control: 1.98 ± 0.07, 2.38 ± 0.14 log mM) to PHE. In the absence of endothelium (E -) lead had no effect but increased baseline perfusion pressure (E + : 79.5 ± 2.4, E -: 118 ± 2.2 mmHg; P < 0.05). To investigate the underlying mechanisms, this protocol was repeated after treatment with 100 µM L-NAME, 10 µM indomethacin and 1 µM tempol in the presence of lead. Lead actions on E max and pD 2 were abolished in the presence of indomethacin, and partially abolished with L-NAME and tempol. Results suggest that acute lead administration affects the endothelium, releasing cyclooxygenasederived vasoconstrictors and involving reactive oxygen species.

show abstract

“…Aliyu et al, [45] observed similar fluctuations. The Mean Corpuscular Haemoglobin Concentration is a good indicator of red blood cell swelling [46], the Mean Corpuscular Haemoglobin Concentration, which is the ratio of haemoglobin concentration as opposed to the haemotocrit, is not influenced by the blood volume for the number of cells in the blood, but can be interpreted incorrectly only when the new cells, with a different haemoglobin concentration, are released into blood circulation. The fluctuation in neutrophils and lymphocytes could possibly be as result of antibodies released in the circulatory system of the mice as a form of defense.…”

Section: Haematological Analysis and Histopathologymentioning

confidence: 99%

The effect of heavy metal air pollution arising from local metallurgical activities on albino rat

Ugya¹,

Imam²

2017

Am J Prev Med Public Health

View full text Add to dashboard Cite

Introduction:The ecological equilibrium of the earth is endangered by the chemical process industries as most industries continue to spew chemical toxicants into the environment. Since local metallurgical activity is inevitable in Nigeria. Aim: This research is aimed at assessing the effect of heavy metals air pollution arising from local metallurgical activities on Albino rat. Methods: Particular matter samples were collected for 24h at four different sites twice a month using a high volume air sampler. Point A is Bakin Dogo Market, Point B is Old Panteka market, Point C is new Panteka market and Point D is U/Ma'azu. All the collected samples were analyzed for heavy metals using standard laboratory procedures. Six hundred newly born male albino rats were collected from National Institution for Trypanosomiasis Research (NITR) and were exposed to the sampling points twenty four hours daily via out the experimental period. Mortality, haematological and histological parameters were investigated. The rat tissues were also digested for the determination of Bioconcentration factor (BCF).Result: The result obtained shows high Concentration of heavy metals (Pb, Cd, Cu and Ni) above the recommended limit by European commission (EC), World health organization (WHO) and NEPM (National Environmental Protection Measures). Higher BCF was obtained for all the heavy metals (Pb, Ni, Cu and Cd) which was attributed to the higher changes in Mortality, haematological and histological parameters if compare to the control. Conclusion:The studies shows how heavy metal air pollution could cause Mortality and also bring about changes in haematological and histological parameters, which resulted due to high BCF. It is thereby recommended that local metallurgical activities should be closely monitored by environmental agencies such as Kaduna state environmental protection agencies (KEPA) and Federal environmental protection agencies (FEPA). The government should also make regulations that will mandate the paying of environmental degradation tax owing to the daily multiplication of vehicles and other automobile materials. ARTICLE HISTORY

show abstract

Cardiotoxicity of lead at various perfusate calcium concentrations: Functional and metabolic responses of the perfused rat heart

Cited by 43 publications

References 30 publications

Lead reduces tension development and the myosin ATPase activity of the rat right ventricular myocardium

Lead reduces tension development and the myosin ATPase activity of the rat right ventricular myocardium

Acute lead-induced vasoconstriction in the vascular beds of isolated perfused rat tails is endothelium-dependent

The effect of heavy metal air pollution arising from local metallurgical activities on albino rat

Contact Info

Product

Resources

About