Cardiotrophin-like cytokine labelling using Bir A biotin ligase: A sensitive tool to study receptor expression by immune and non-immune cells

Cognet, Isabelle; Guilhot, Joëlle; Gabriac, Mélanie; Chevalier, Sylvie; Chouikh, Younes; Herman-Bert, Alexandra; Guay-Giroux, Angélique; Corneau, Sophie; Magistrelli, Giovanni; Elson, Greg; Gascan, Hugues; Gauchat, Jean‐François

doi:10.1016/j.jim.2005.03.012

Cited by 11 publications

(9 citation statements)

References 54 publications

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“…It has been suggested that a 3rd potential receptor is utilized by CLCF1 rather than CNTFR since binding of CLCF1 to B cells has been confirmed [14]. This postulated receptor remains to be identified.…”

Section: Compound Cytokine Formation: Clcf1/crlf1 Clcf1/scntfr and Pmentioning

confidence: 98%

Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders

Sims

2015

Cytokine & Growth Factor Reviews

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Section: Compound Cytokine Formation: Clcf1/crlf1 Clcf1/scntfr and Pmentioning

confidence: 98%

Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders

Sims

2015

Cytokine & Growth Factor Reviews

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“…The CLF cDNA (23) was modified by introducing a sequence coding for a FLAG epitope (DYKDDDD) at the COOH terminus of the protein. The p28-protC and CLF-FLAG cDNAs were cloned in the vector pcDNA5 (Invitrogen) alone or as a bicistronic insert containing an internal ribosome entry site and used to generate stable Flp-In-293 (Invitrogen) transfectants as previously described (24).…”

Section: Generation Of Stable Hek-293 Transfectants Expressing Human mentioning

confidence: 99%

“…Flp-In-293 transfectants expressing human p28-protC, CLF-FLAG, and p28-protC/CLF-FLAG were grown as described elsewhere (24). Supernatants were concentrated by ultrafiltration and incubated overnight at 4°C with anti-protein C agarose (Roche Applied Sciences) or anti-FLAG M2-agarose (Sigma-Aldrich).…”

Section: Immunoprecipitations and Western Blottingmentioning

confidence: 99%

The IL-27 p28 Subunit Binds Cytokine-Like Factor 1 to Form a Cytokine Regulating NK and T Cell Activities Requiring IL-6R for Signaling

Crabé

Guay-Giroux

Tormo

et al. 2009

The Journal of Immunology

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125

142

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IL-27 is formed by the association of a cytokine subunit, p28, with the soluble cytokine receptor EBV-induced gene 3 (EBI3). The IL-27R comprises gp130 and WSX-1. The marked difference between EBI3−/− and WSX-1−/− mice suggests that p28 has functions independent of EBI3. We have identified an alternative secreted complex formed by p28 and the soluble cytokine receptor cytokine-like factor 1 (CLF). Like IL-27, p28/CLF is produced by dendritic cells and is biologically active on human NK cells, increasing IL-12- and IL-2-induced IFN-γ production and activation marker expression. Experiments with Ba/F3 transfectants indicate that p28/CLF activates cells expressing IL-6Rα in addition to the IL-27R subunits. When tested on CD4 and CD8 T cells, p28/CLF induces IL-6Rα-dependent STAT1 and STAT3 phosphorylation. Furthermore, p28/CLF inhibits CD4 T cell proliferation and induces IL-17 and IL-10 secretion. These results indicate that p28/CLF may participate in the regulation of NK and T cell functions by dendritic cells. The p28/CLF complex engages IL-6R and may therefore be useful for therapeutic applications targeting cells expressing this receptor. Blocking IL-6R using humanized mAbs such as tocilizumab has been shown to be beneficial in pathologies like rheumatoid arthritis and juvenile idiopathic arthritis. The identification of a new IL-6R ligand is therefore important for a complete understanding of the mechanism of action of this emerging class of immunosuppressors.

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“…It activates a complex receptor consisting of gp130, ciliary neurotrophic factor receptor (CNTFR α ), and leukemia inhibitory factor receptor (LIFR α ) [4]. An alternative receptor has been detected on B cells by the use of labeled CLCF-1 but has not been completely characterized [31]. The requirements for interactions between CLCF-1 and related cytokines and specific receptors are under active investigation.…”

Section: Discussionmentioning

confidence: 99%

Renal and Hematological Effects of CLCF-1, a B-Cell-Stimulating Cytokine of the IL-6 Family

Savin

Sharma

Zhou

et al. 2015

Journal of Immunology Research

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CLCF-1 is a cytokine known for B-cell stimulation and for neurotrophic properties. We have identified CLCF-1 as a potential injurious factor in the human renal disease focal segmental glomerulosclerosis (FSGS). We investigated its effects on renal cells and renal function in in vitro and in vivo studies. Methods include measurement of the effect of CLCF-1 on phosphorylation of target molecules of the JAK/STAT pathway, on cytoskeleton and cell morphology in cultured podocytes, on albumin permeability of isolated rat glomeruli, and on tissue phosphorylation and urine albumin after acute or chronic CLCF-1 injection. In addition, cell sorting was performed to determine the presence of cells expressing CLCF-1 in spleen and bone marrow of normal mice and the effect of CLCF-1 infusion on splenic B-cell populations. CLCF-1 increased phosphorylation of STAT3 in multiple cell types, activated podocytes leading to formation of lamellipodia and decrease in basal stress fibers, increased glomerular albumin permeability, and increased STAT3 phosphorylation of peripheral blood cells and renal cortex. CLCF-1 increased urine albumin/creatinine ratio in mice and increased B-cell expression of IgG in mouse spleen. We conclude that CLCF-1 has potentially important systemic effects, alters podocyte function, and may contribute to renal dysfunction and albuminuria.

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Cardiotrophin-like cytokine labelling using Bir A biotin ligase: A sensitive tool to study receptor expression by immune and non-immune cells

Cited by 11 publications

References 54 publications

Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders

Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders

The IL-27 p28 Subunit Binds Cytokine-Like Factor 1 to Form a Cytokine Regulating NK and T Cell Activities Requiring IL-6R for Signaling

Renal and Hematological Effects of CLCF-1, a B-Cell-Stimulating Cytokine of the IL-6 Family

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