Cardiovascular Risk Factors and Total Serum Antioxidant Capacity in Healthy Men and in Men with Coronary Heart Disease

Abstract

Whether the incidence of coronary heart disease (CHD) is related to a decrease in total antioxidant capacity (TAC) has not yet been completely clarified. We assessed TAC of blood serum in a group of 163 men with CHD aged 34.8–77.0 years and in 163 age-matched peers without CHD. Two spectrophotometric methods were applied to assess TAC: ferric reducing ability of serum (TAC-FRAS) and 2.2-diphenyl-1-picryl-hydrazyl (TAC-DPPH) tests. In the CHD group, multivariate analysis revealed that uric acid (UA), triglycerides, and systolic blood pressure contributed independently to the TAC-FRAS variance. TAC-DPPH was favorably predicted by UA concentration, but negatively so by current smoking and glucose levels. In men without CHD, UA was the only independent determinant of both TAC-FRAS and TAC-DPPH. Presence of CHD was not an independent predictor of TAC—observed between-group differences (higher TAC in CHD patients) disappeared after adjustment for other confounders. We conclude that UA is the main determinant of TAC of blood serum in men. TAC is not directly influenced by age or CHD but is related to several indices of overweight/obesity and laboratory measures of metabolic syndrome, especially in patients with CHD.

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“…In fact, the sulfhydryl groups in Hcy were oxidized to disulfide, catalyzed by the transition metals which product several OFR (hydroperoxides: H 2 O 2 ), and initiates lipid peroxidation responsible of endothelial injury 11,28 . Erythrocyte SOD activity was significantly decreased in IHD cases wich is in agreement with several studies such as those reported by Almzaiel AJT et al 14,29,30 . SOD, FR scavenging enzyme, are the first line of cellular defense against oxidative injury 20 .…”

A comparison of native and non-urate Total Antioxidant Capacity of fasting plasma and saliva among middle-aged and older subjects

“…Plasma TAC measurements were performed using two spectrophotometric methods: FRAP [8] with some modifications [12], and the DPPH test [12,14]. The details of both methods are described elsewhere [12,16]. Both tests were executed with native plasma samples (containing UA) and pretreated with uricase and catalase (with decomposed UA), as described elsewhere [15].…”

Peritoneal and Systemic Responses of Obese TypeIIDiabetic Rats to Chronic Exposure to a Hyperbranched Polyglycerol‐Based Dialysis Solution

“…Uric acid is a major antioxidant in serum . The level of antioxidant activity in each experimental group was evaluated by measuring the serum uric acid level.…”

“…In addition to the serum cytokine profile, the change in the antioxidant capacity of plasma is related to MetS . Many serum molecules contribute to the serum antioxidant activity, and uric acid is one of the major antioxidants of serum antioxidant activity with MetS .…”