Cargo-loading of hybrid cowpea chlorotic mottle virus capsids via a co-expression approach

Timmermans, Suzanne B. P. E.; Mesman, Rob; Blezer, Kim J.R.; Niftrik, Laura van; Hest, Jan C. M. van

doi:10.1016/j.virol.2022.10.011

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…Plant viruses and their respective virus-like particles (VLP) offer an exciting nanotechnological platform for a variety of applications. Their propensity to self-assemble into monodisperse and highly symmetric nanometer-range particles makes them obvious candidates for the entrapment and delivery of biomedically active cargos in vivo [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. In addition, the rigid protein capsids may be genetically or chemically modified to introduce (bio)chemical moieties to be displayed on the viruses’ surface [ 11 , 12 , 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Efficient Purification of Cowpea Chlorotic Mottle Virus by a Novel Peptide Aptamer

Tscheuschner

Ponader

Raab

et al. 2023

Viruses

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The cowpea chlorotic mottle virus (CCMV) is a plant virus explored as a nanotechnological platform. The robust self-assembly mechanism of its capsid protein allows for drug encapsulation and targeted delivery. Additionally, the capsid nanoparticle can be used as a programmable platform to display different molecular moieties. In view of future applications, efficient production and purification of plant viruses are key steps. In established protocols, the need for ultracentrifugation is a significant limitation due to cost, difficult scalability, and safety issues. In addition, the purity of the final virus isolate often remains unclear. Here, an advanced protocol for the purification of the CCMV from infected plant tissue was developed, focusing on efficiency, economy, and final purity. The protocol involves precipitation with PEG 8000, followed by affinity extraction using a novel peptide aptamer. The efficiency of the protocol was validated using size exclusion chromatography, MALDI-TOF mass spectrometry, reversed-phase HPLC, and sandwich immunoassay. Furthermore, it was demonstrated that the final eluate of the affinity column is of exceptional purity (98.4%) determined by HPLC and detection at 220 nm. The scale-up of our proposed method seems to be straightforward, which opens the way to the large-scale production of such nanomaterials. This highly improved protocol may facilitate the use and implementation of plant viruses as nanotechnological platforms for in vitro and in vivo applications.

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Section: Introductionmentioning

confidence: 99%

Efficient Purification of Cowpea Chlorotic Mottle Virus by a Novel Peptide Aptamer

Tscheuschner

Ponader

Raab

et al. 2023

Viruses

View full text Add to dashboard Cite

show abstract

“…MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions, or products referred to in the content. monodisperse and highly symmetric nanometer-range particles makes them obvious candidates for the entrapment and delivery of biomedically active cargos in vivo [1][2][3][4][5][6][7][8][9][10]. In addition, the rigid protein capsids may be genetically or chemically modified to introduce (bio)chemical moieties to be displayed on the viruses' surface [11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Efficient Purification of Cowpea Chlorotic Mottle Virus by a Novel Peptide Aptamer

Tscheuschner¹,

Ponader²,

Raab³

et al. 2023

Preprint

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Cowpea chlorotic mottle virus (CCMV) is a plant virus explored as a nanotechnological platform. The robust self-assembly mechanism of its capsid protein allows for drug encapsulation and targeted delivery. Additionally, the capsid nanoparticle can be used as a programmable platform to display different molecular moieties. In view of future applications, efficient production and purification of plant viruses is a key step. In established protocols, the need for ultracentrifugation is a significant limitation due to cost, difficult scalability, and safety issues. In addition, the purity of the final virus isolate often remains unclear. Here, an advanced protocol for the purification of CCMV from infected plant tissue was developed, focusing on efficiency, economy, and final purity. The protocol involves precipitation with PEG 8000, followed by an affinity extraction using a novel peptide aptamer. The efficiency of the protocol was validated using size exclusion chromatography, MALDI-TOF mass spectrometry, reversed-phase HPLC, and sandwich immunoassay. It was demonstrated that the final eluate of the affinity column is of exceptional purity (98.4%) determined by HPLC and detection at 220 nm. The scale-up of our proposed method seems to be straightforward, which opens the way to the large-scale production of such nanomaterials. This highly improved protocol may facilitate the use and implementation of plant viruses as nanotechnological platforms for in vitro and in vivo applications.

show abstract

Viral nanoparticles: Current advances in design and development

et al. 2024

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Cargo-loading of hybrid cowpea chlorotic mottle virus capsids via a co-expression approach

Cited by 5 publications

References 37 publications

Efficient Purification of Cowpea Chlorotic Mottle Virus by a Novel Peptide Aptamer

Efficient Purification of Cowpea Chlorotic Mottle Virus by a Novel Peptide Aptamer

Efficient Purification of Cowpea Chlorotic Mottle Virus by a Novel Peptide Aptamer

Viral nanoparticles: Current advances in design and development

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