Cariostatic Effect of Palatinose on Experimental Dental Caries in Rats

Ooshima, Takashi; Izumitani, A; Sobue, S; Hamada, Shigeyuki

doi:10.7883/yoken1952.36.219

Cited by 72 publications

(59 citation statements)

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Section: Methodsmentioning

confidence: 99%

“…The amplified fragments were visualized in 0.7 % agarose gel in the presence of 1 mg ethidium bromide ml 21 . For all detections, strains TW871 and MT8148 (Fujiwara et al, 2001;Ooshima et al, 1983) were used as positive and negative controls, respectively.Analysis of clonal relationship using MLST. One S. mutans strain possessing the cnm gene from each subject was randomly selected and analysed by the MLST scheme, as described previously (Nakano et al, 2007).…”

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Multilocus sequence typing analysis of Streptococcus mutans strains with the cnm gene encoding collagen-binding adhesin

Lapirattanakul

Nakano

Nomura

et al. 2011

Journal of Medical Microbiology

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Streptococcus mutans is one of the oral pathogens associated with infective endocarditis (IE).With respect to bacterial binding ability to the extracellular matrix, the Cnm protein, a cell surface collagen-binding adhesin of S. mutans, is known as one of the possible virulence factors with regard to IE. In this study, we aimed to determine the distribution of the cnm gene, which encodes Cnm, in a large number of clinical isolates of S. mutans from Thai subjects. Then, the cnm-positive strains were classified using a multilocus sequence typing (MLST) scheme, which we constructed previously. In addition, the data were analysed together with our previous MLST data of cnmpositive strains from Japan and Finland in order to evaluate the clonal relationship among S. mutans strains harbouring the cnm gene. The cnm gene was detected in 12.4 % of all 750 Thai isolates, and serotype f showed the highest rate of detection (54.5 %). According to the MLST data, two clonal complex groups were revealed as the important clones related to cnm-positive S. mutans from various origins of isolation. Moreover, the collagen-binding properties of S. mutans strains with the cnm gene were significantly greater than those of strains without the gene, although four cnm-negative strains classified into two sequence types (STs), ST110 and ST136, showed extremely high collagen-binding rates suggesting the presence of additional genes involved with collagen binding in these STs. Taken together, these results provided information on both epidemiological as well as evolutional aspects of S. mutans possessing the cnm gene. INTRODUCTIONStreptococcus mutans is a well-known pathogen of dental caries (Hamada & Slade, 1980), which consists of four serotypes, c, e, f and k (Linzer et al., 1986;). The detection rate of serotype c is highest among the strains isolated from oral cavities, followed by serotype e, while serotypes f and k are regarded as being present in minor proportions . Various pieces of evidence show the association of S. mutans with some life-threatening diseases such as infective endocarditis (IE), although the major species related to IE belong to the mitis group of oral streptococci (Banas, 2004;Mylonakis & Calderwood, 2001). Moreover, continuous detections of this bacterium in specimens from patients suffering from such disease emphasize the importance of S. mutans in IE (Gauduchon et al., 2001;Nomura et al., 2006;Vose et al., 1987;Ullman et al., 1988). Indeed, studies to clarify specific components involved in IE in S. mutans have also been performed worldwide (Beg et al., 2002;Jung et al., 2009; MatsumotoNakano et al., 2009;Miller-Torbert et al., 2008).IE is initiated by the binding of bacterial surface molecules to host extracellular matrix (ECM) proteins exposed on damaged heart tissue (Moreillon & Que, 2004). In S. mutans, collagen-binding adhesin or Cnm protein, encoded by the cnm gene, was reported as a strain-specific collagen-binding molecule (Sato et al., 2004). The Cnm protein possesses a collagen-binding domain (CBD) in ...

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Multilocus sequence typing analysis of Streptococcus mutans strains with the cnm gene encoding collagen-binding adhesin

Lapirattanakul

Nakano

Nomura

et al. 2011

Journal of Medical Microbiology

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“…® e Palatinit ® , tem atraí-do a atenção de pesquisadores devido a sua aplicação industrial como substituto da sacarose em processamento de alimentos, sendo utilizado como adoçante dietético, não cariogênico e em formulações farmacêuticas [13][14][15] . Muitos produtos derivados da isomaltulose possuem potenciais aplicações industriais.…”

Section: -O-(α-d-glicopiranosil)-d-sorbitol] E [6-o-(α-dglicopiranosiunclassified

“…A sín-tese de glucana promove a aderência de Streptococcus mutans às superfícies sólidas, incluindo aquelas dos dentes. Estes microrganismos são capazes de metabolizar mono e dissacarídeos produzindo ácidos, provocando a queda do pH da saliva e na interface placa-esmalte dos dentes favorecendo a desmineralização do esmalte e, conseqüentemente, desenvolvendo a cárie 14,78 . O interesse na produção de isômeros da sacarose tem aumentado devido às similaridades sensoriais e ao menor potencial cariogênico.…”

Section: Aplicações Da Isomaltuloseunclassified

Produção de isomaltulose, um substituto da sacarose, utilizando glicosiltransferase microbiana

Kawaguti¹,

Sato²

2008

Quím. Nova

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“…Until recently (Thompson et al, 2001a), there were no reports of the bacterial utilization of the five sucrose isomers. Indeed, the inability of mutans streptococci to metabolize these comparatively sweet disaccharides suggests their use as non-cariogenic substitutes for dietary sucrose (Ooshima et al, 1983(Ooshima et al, , 1991Ziesenitz et al, 1989 ;Minami et al, 1990 ;Peltroche-Llacsahuanga et al, 2001). In light of these reports, we were surprised to discover that K. pneumoniae readily utilized all five α--glucosyl--fructoses as energy sources for growth (Thompson et al, 2001a, b), and that the enzymes encoded by the sucrose (scr) operon (Titgemeyer et al, 1996) did not participate in dissimilation of these compounds.…”

Section: Introductionmentioning

confidence: 99%

Metabolism of sucrose and its five isomers by Fusobacterium mortiferum

et al. 2002

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Fusobacterium mortiferum utilizes sucrose [glucose-fructose in α(1 2) linkage] and its five isomeric α-D-glucosyl-D-fructoses as energy sources for growth.Sucrose-grown cells are induced for both sucrose-6-phosphate hydrolase (S6PH) and fructokinase (FK), but the two enzymes are not expressed above constitutive levels during growth on the isomeric compounds. Extracts of cells grown previously on the sucrose isomers trehalulose α(1 1), turanose α(1 3), maltulose α(1 4), leucrose α(1 5) and palatinose α(1 6) contained high levels of an NAD M plus metal-dependent phospho-α-glucosidase (MalH). The latter enzyme was not induced during growth on sucrose. MalH catalysed the hydrolysis of the 6'-phosphorylated derivatives of the five isomers to yield glucose 6-phosphate and fructose, but sucrose 6-phosphate itself was not a substrate. Unexpectedly, MalH hydrolysed both α-and β-linked stereomers of the chromogenic analogue p-nitrophenyl glucoside 6-phosphate. The gene malH is adjacent to malB and malR, which encode an EII(CB) component of the phosphoenolpyruvate-dependent sugar :phosphotransferase system and a putative regulatory protein, respectively. The authors suggest that for F. mortiferum, the products of malB and malH catalyse the phosphorylative translocation and intracellular hydrolysis of the five isomers of sucrose and of related α-linked glucosides. Genes homologous to malB and malH are present in both Klebsiella pneumoniae and the enterohaemorrhagic strain Escherichia coli O157 :H7. Both these organisms grew well on sucrose, but only K. pneumoniae exhibited growth on the isomeric compounds.

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Cariostatic Effect of Palatinose on Experimental Dental Caries in Rats

Cited by 72 publications

References 10 publications

Multilocus sequence typing analysis of Streptococcus mutans strains with the cnm gene encoding collagen-binding adhesin

Multilocus sequence typing analysis of Streptococcus mutans strains with the cnm gene encoding collagen-binding adhesin

Produção de isomaltulose, um substituto da sacarose, utilizando glicosiltransferase microbiana

Metabolism of sucrose and its five isomers by Fusobacterium mortiferum

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