Carious deciduous teeth are a potential source for dental pulp stem cells

Werle, Stefanie Bressan; Lindemann, Daniele; Steffens, Daniela; Demarco, Flávio Fernando; Araújo, Fernando Borba de; Pranke, Patrícia; Casagrande, Luciano

doi:10.1007/s00784-015-1477-5

Cited by 56 publications

(46 citation statements)

References 26 publications

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“…Werle et al . () reported a successful DPSC isolation rate of 70% for carious primary teeth and 100% for healthy primary teeth. These authors did not find any contamination of culture.…”

Section: Discussionmentioning

confidence: 99%

Odontoblastic differentiation of dental pulp stem cells from healthy and carious teeth on an original PCL‐based 3D scaffold

et al. 2017

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“…Werle et al . () reported a successful DPSC isolation rate of 70% for carious primary teeth and 100% for healthy primary teeth. These authors did not find any contamination of culture.…”

Section: Discussionmentioning

confidence: 99%

Odontoblastic differentiation of dental pulp stem cells from healthy and carious teeth on an original PCL‐based 3D scaffold

et al. 2017

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“…Previous studies have shown that hDPCs can differentiate to a matrix mineralization phenotype (Gronthos et al, 2000, 2002; d'Aquino et al, 2007; El-Gendy, 2010), while controversial data have been published regarding cDPCs isolated from permanent (Alongi et al, 2010; Wang et al, 2010; Pereira et al, 2012; Ma et al, 2014; Yazid et al, 2014) or deciduous (Yu et al, 2014; Werle et al, 2015) teeth affected by deep caries. Our data clearly show that cDPCs isolated from shallow carious lesions can differentiate to this phenotype and form a mineralized matrix (Figure 3).…”

Section: Discussionmentioning

confidence: 99%

Dental Pulp Cells Isolated from Teeth with Superficial Caries Retain an Inflammatory Phenotype and Display an Enhanced Matrix Mineralization Potential

et al. 2017

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We have isolated dental pulp cells (DPCs) from three healthy (hDPCs) and three carious (cDPCs) donors and shown that compared to hDPCs cells isolated from superficial carious lesions show higher clonogenic potential; show an equivalent proportion of cells with putative stem cell surface markers; show enhanced matrix mineralization capability; have enhanced angiogenic marker expression and retain the inflammatory phenotype in vitro characteristic of superficial caries lesions in vivo. Our findings suggest that cDPCs may be used for further investigation of the cross talk between inflammatory, angiogenic and mineralization pathways in repair of carious pulp. In addition cells derived from carious pulps (almost always discarded) may have potential for future applications in mineralized tissue repair and regeneration.

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“…compared the enzyme digestion and outgrowth methods and found that cells isolated by enzyme digestion had a higher proliferation rate than those isolated by the other methods. In this study, the enzymatic digestion method used in the studies of Yu, et al [13], Bernardi, et al [14], Werle, et al [2], and their colleagues, was employed.…”

Section: Discussionmentioning

confidence: 99%

“…To evaluate the ability of the cells to differentiate, 10 4 cells/cm 2 (at the fifth passage) were plated in 12-well plates and cultivated in appropriate media osteogenic, adipogenic and chondrogenic differentiation for 2 to 4 weeks after reaching at least 70% confluence [2]. For each experiment, a negative control was used consisting of the same cells maintained in conventional culture medium.…”

Section: Cell Differentiation In Vitromentioning

confidence: 99%

Isolation, immunophenotypic characterization and pluripotency of dental pulp stem cells

Luisi¹,

Filho²,

Pranke³

2017

Dent Oral Craniofac Res

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Objective: The aim of this study has been the isolation and characterization of stem cells from dental pulp (DPSCs) in culture. Methods:The primary DPSCs cultures were obtained from human third molars. Immediately after extraction, the teeth were placed in Dulbecco's modified Eagle medium (DMEM) culture medium supplemented with fetal bovine serum and antibiotics. In a laminar flow, the pulp was removed from the tooth, incubated with collagenase for 2 hours and placed on a culture plate. Phenotypic characterization and cell pluripotency was performed in the fifth passage (P5). To evaluate the expression of surface markers, the cells were incubated with antibodies against CD14, CD29, CD34, CD44, CD45, CD73, CD90, CD105 and HLA-DR antigens. For induction of cell differentiation in vitro, 10 4 cells/cm 2 were plated in 12-well plates and cultivated in appropriate media for osteogenic, adipogenic and condrogencic differentiation after reaching at least 70% confluence. Results:The cells were positive above 95% for characteristic markers of the mesenchymal stem cells CD29, CD44, CD73 and CD90. In contrast, there was a low percentage of positivity (up to 1.1%) for the characteristic markers of hematopoietic cells such as CD14, CD34, CD45, CD184 and HLA-DR. Adipogenic differentiation was visualized by staining lipid vacuoles with Oil Red. Bone differentiation was visualized by staining calcium deposits with Alizarin Red. No chondrogenic differentiation was observed. Conclusions:The isolated cells were adherent to plastic, positive for the characteristic markers of mesenchymal stem cells and, therefore, an alternative source for tissue engineering studies.

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Carious deciduous teeth are a potential source for dental pulp stem cells

Abstract: Decayed deciduous teeth have been usually discarded once the pulp tissue could be damaged and the activity of stem cells compromised. These findings show that stem cells from carious deciduous teeth can be applicable source for cell-based therapies in tissue regeneration.

Cited by 56 publications

References 26 publications

Odontoblastic differentiation of dental pulp stem cells from healthy and carious teeth on an original PCL‐based 3D scaffold

Odontoblastic differentiation of dental pulp stem cells from healthy and carious teeth on an original PCL‐based 3D scaffold

Dental Pulp Cells Isolated from Teeth with Superficial Caries Retain an Inflammatory Phenotype and Display an Enhanced Matrix Mineralization Potential

Isolation, immunophenotypic characterization and pluripotency of dental pulp stem cells

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