Carotene production from waste cooking oil by <i>Blakeslea trispora</i> in a bubble column reactor: The role of oxidative stress

Nanou, Konstantina; Roukas, T.; Papadakis, Emmanouil-Nikolaos; Kotzekidou, P.

doi:10.1002/elsc.201600228

Cited by 12 publications

(9 citation statements)

References 34 publications

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“…The highest yield (2 gÁL À1 ) was obtained using WCO as sole carbon source supplemented with corn steep liquor and butylated hydroxytoluene, and in this condition the carotenes produced consisted of b-carotene (74.2 %), c-carotene (23.2 %) and lycopene (2.6 %). Upscaling the process to a 1.4-L bubble column reactor did not favored the production of carotenes and lower amount was attained comparatively to flask experiments (Nanou, Roukas, Papadakis, & Kotzekidou, 2017).…”

Section: Other Compoundsmentioning

confidence: 74%

Microbial valorization of waste cooking oils for valuable compounds production – a review

Lopes

Miranda

Belo

2019

Critical Reviews in Environmental Science and Technology

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Waste cooking oils (WCO) are vegetable oils discarded after food frying and great amounts are produced worldwide. Its management is a challenge, due to the environmental risk of illegally disposal into rivers and landfills. The main approaches for WCO valorization included their incorporation as component of animal feed and biodiesel manufacturing. Yet, the development of new feasible approaches is attractive from an economic and ecological standpoint. Due to their composition in triglycerides, untreated WCO can be used as feedstock for microbial growth (several species are able to use them as carbon source) and production of added-value compounds. In this way, microbial valorization of WCO is a sustainable biotechnological approach to upgrade a waste into a renewable feedstock for bio-based industry, favoring the circular economy concept. The objective of this review is to highlight the potential use of WCO in bioprocesses as an alternative to other physicochemical treatments. Firstly, an introduction to WCO problematic is presented, describing most common applications used currently. Then, an extensive review on the use of WCO by microorganisms is shown, focusing on bacterial and fungi species and its exploitation for bioprocesses development to produce metabolites of industrial interest, such as biopolymers, biosurfactants, lipases and microbial lipids. KEYWORDS Added-value compounds; microbial conversion; waste cooking oils 1. Waste cooking oils: General overview Waste cooking oils (WCO) are generated from vegetable oils (coconut, sunflower, soybean, palm tree, cottonseed, rapeseed, olive, etc.) employed to fry foods in household and HORECA (Hotels, Restaurants and Catering) segments and are no longer suitable for human consumption. Specifically, in HORECA sector, fast food restaurants (particularly those of chicken and

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Section: Other Compoundsmentioning

confidence: 74%

Microbial valorization of waste cooking oils for valuable compounds production – a review

Lopes

Miranda

Belo

2019

Critical Reviews in Environmental Science and Technology

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“…Therefore, carotenoids do not play a major physiological role in fungal cells, but they may have beneficial effects under specific conditions. Several studies showed an increase in carotenoid concentration under stress [45,57]. The potential photoprotective pigment torulene [9,58] showed low concentrations in K. petricola A95, which would imply a less important role in K. petricola A95, but an inducible production under a stress situation out of the more stable dehydrolycopene seems possible.…”

Section: Discussionmentioning

confidence: 99%

Development of an Improved Carotenoid Extraction Method to Characterize the Carotenoid Composition under Oxidative Stress and Cold Temperature in the Rock Inhabiting Fungus Knufia petricola A95

Flieger

Knabe

Toepel

2018

JoF

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Black yeasts are a highly specified group of fungi, which are characterized by a high resistance against stress factors. There are several factors enabling the cells to survive harsh environmental conditions. One aspect is the pigmentation, the melanin black yeasts often display a highly diverse carotenoid spectrum. Determination and characterization of carotenoids depend on an efficient extraction and separation, especially for black yeast, which is characterized by thick cell walls. Therefore, specific protocols are needed to ensure reliable analyses regarding stress responses in these fungi. Here we present both. First, we present a method to extract and analyze carotenoids and secondly we present the unusual carotenoid composition of the black yeast Knufia petricola A95. Mechanical treatment combined with an acetonitrile extraction gave us very good extraction rates with a high reproducibility. The presented extraction and elution protocol separates the main carotenoids (7) in K. petricola A95 and can be extended for the detection of additional carotenoids in other species. K. petricola A95 displays an unusual carotenoid composition, with mainly didehydrolycopene, torulene, and lycopene. The pigment composition varied in dependency to oxidative stress but remained relatively constant if the cells were cultivated under low temperature. Future experiments have to be carried out to determine if didehydrolycopene functions as a protective agent itself or if it serves as a precursor for antioxidative pigments like torulene and torularhodin, which could be produced after induction under stress conditions. Black yeasts are a promising source for carotenoid production and other substances. To unravel the potential of these fungi, new methods and studies are needed. The established protocol allows the determination of carotenoid composition in black yeasts.

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“…Another explanation for the stable carotenoid concentrations in K. petricola A95 would be that the mechanism of carotenoid action is more likely to consist of shielding sensitive molecules or organelles than of neutralization of harmful oxidants [51]. under stress [43,52]. The potential photoprotective pigment torulene [6,53] showed low concentrations in K.…”

Section: Discussionmentioning

confidence: 99%

Development of an Improved Carotenoid Extraction Method and Characterisation of the Carotenoid Composition under Oxidative and Cold Stress in the Rock Inhabiting Fungus <em>Knufia Petricola</em> A95

Flieger¹,

Knabe²,

Toepel³

2018

Preprint

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Black yeasts are a highly specified group of fungi, which are characterized by a high resistance against stress factors. There are several factors enabling the cells to survive harsh environmental conditions. One aspect is the pigmentation, besides the melanin black yeasts often display a highly diverse carotenoid spectrum. Determination and characterization of carotenoids depend on an efficient extraction and separation, therefore especially for black yeast, characterized by thick cell walls specific protocols are needed to ensure analyses regarding stress responses in these fungi. Here we present both, a method to extract and analyze carotenoids and the unusual carotenoid composition of the black yeast Knufia petriola A95. Mechanical treatment combined with an acetonitrile extraction gave us very good extraction rates with a high reproducibility. The presented extraction and elution protocol allows the separation of the main carotenoids (7) in K. petricola A95 and should be suitable for the detection of additional carotenoids in other species. K. petricola A95 displays an unusual carotenoid composition, with mainly didehydrolycopene, torulene and lycopene. The pigment composition varied in dependency to oxidative stress but remained relatively constant if the cells were cultivated under low temperature. Black yeasts are a promising source for carotenoid production and other substances. To unravel the potential of these fungi new methods and studies are needed. The established protocol allows the determination carotenoid composition in black yeasts. Oxidative stress results in an adaptation in pigment composition in K. petricola A95. Future experiments have to be carried out to determine if didehydrolycopene functions as a protective agent itself or if it serves as a precursor for antioxidative pigments like torulene and torularhodin, which could be produced after induction under stress conditions.

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Carotene production from waste cooking oil by Blakeslea trispora in a bubble column reactor: The role of oxidative stress

Cited by 12 publications

References 34 publications

Microbial valorization of waste cooking oils for valuable compounds production – a review

Microbial valorization of waste cooking oils for valuable compounds production – a review

Development of an Improved Carotenoid Extraction Method to Characterize the Carotenoid Composition under Oxidative Stress and Cold Temperature in the Rock Inhabiting Fungus Knufia petricola A95

Development of an Improved Carotenoid Extraction Method and Characterisation of the Carotenoid Composition under Oxidative and Cold Stress in the Rock Inhabiting Fungus <em>Knufia Petricola</em> A95

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