Carotenoid Analytical Methods

Ritter, E. De; Purcell, Albert E.

doi:10.1016/b978-0-12-082850-0.50017-2

Cited by 97 publications

(52 citation statements)

References 393 publications

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“…where the molar extinction coefficient of 17.2 Â 10 4 /M/cm is that reported by for lycopene in hexane. Most extinction coefficients that have been reported subsequently are within 1-2% of this value (e.g., DeRitter and Purcell, 1981). We chose to work with values of lycopene content expressed in terms of mg/kg since that (or the equivalent, mg/g) makes data handling easier and is a unit of concentration commonly used in the literature (Beerh and Siddappa, 1959;Perkins-Veazie et al, 2001).…”

Section: Reduced Volume Lycopene Assaymentioning

confidence: 99%

A Quantitative Assay for Lycopene That Utilizes Reduced Volumes of Organic Solvents

Fish

Perkins-Veazie

Collins

2002

Journal of Food Composition and Analysis

462

252

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Section: Reduced Volume Lycopene Assaymentioning

confidence: 99%

A Quantitative Assay for Lycopene That Utilizes Reduced Volumes of Organic Solvents

Fish

Perkins-Veazie

Collins

2002

Journal of Food Composition and Analysis

462

252

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“…Extracts were cleared by centrifugation, and absorption spectra were recorded against the solvent in a Hewlett-Packard model 8452A diode array spectrophotometer. Bacterioruberin was estimated according to the absorbance at 498 nm, using a El value of 2540 for a-bacterioruberin (De Ritter & Purcell, 1981), when necessary after correction for the contribution of carotenoids derived from Dunaliella parva cells to the overall absorption . Bacteriorhodopsin was quantified as described by Oren & Shilo (1981) .…”

Section: Protein and Pigment Analysesmentioning

confidence: 99%

Dynamics of a bloom of halophilic archaea in the Dead Sea

Oren

Гуревич

1995

Hydrobiologia

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After a period of more than ten years in which bacterial and algal community sizes were extremely small, a dense bloom of halophilic archaea developed in the upper 5-10 m of the Dead Sea water column in the summer of 1992 . The development of this bloom followed a dilution of the upper water layer by winter rainfloods, which enabled the development of a short-lived dense bloom of the unicellular green alga Dunaliella parva . The dense archaeal community (up to 3 .5 x 107 cells ml -1 in June 1992) imparted a red coloration to the Dead Sea, due to its high content of bacterioruberin . Bacteriorhodopsin was not detected . High levels of potential heterotrophic activity were associated with the bloom, as measured by the incorporation of labeled organic substrates . After the decline of the algal bloom, archaeal numbers in the lake decreased only little, and most of the community was still present at the end of 1993 . The amount of carotenoid pigment per cell, however, decreased 2-3-fold between

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“…Calculations were made by using the extinction coefficient given by De Ritter and Purcell(1981). The carotenoid yield was expressed as mg carotenoids per kg peel on a dry basis.…”

Section: Methodsmentioning

confidence: 99%

Utilisation of orange by‐products—orange peel carotenoids

et al. 1992

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Carotenoids were extracted from fresh orange peel with various solvents. Acetone was the most efficient of the solvents tested. Two successive extractions with acetone after an initial washing with either acetone or methanol were adequate to remove 89% of the total carotenoids. The extracts were concentrated, the carotenoids transferred to hexane and a crude pigment concentrate was obtained by hexane evaporation. Water washings prior to acetone extraction eliminated the solvent-solvent transfer to hexane. The extraction residue was used for pectin recovery. Carotenoid removal from the peel did not affect the yield and quality of the pectin.

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Carotenoid Analytical Methods

Cited by 97 publications

References 393 publications

A Quantitative Assay for Lycopene That Utilizes Reduced Volumes of Organic Solvents

A Quantitative Assay for Lycopene That Utilizes Reduced Volumes of Organic Solvents

Dynamics of a bloom of halophilic archaea in the Dead Sea

Utilisation of orange by‐products—orange peel carotenoids

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