Carotenoid Biosynthesis during Tomato Fruit Development (Evidence for Tissue-Specific Gene Expression)

Fraser, Paul D.; Truesdale, Mark R.; Bird, Colin R.; Schuch, Wolfgang; Bramley, Peter M.

doi:10.1104/pp.105.1.405

Cited by 481 publications

(387 citation statements)

References 23 publications

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“…We therefore hypothesize that LeGGPS1 produces substrate for TMTT biosynthesis. It is possible that LeGGPS2 provides GGPP for the production of the carotenoids in fruits, which increases from 2.2 g/g FW in green fruit to 189 g/g FW in red fruit (Fraser et al 1994). The TIGR tomato EST database contains several ESTs encoding other putative GGPP synthases.…”

Section: Leggps1 Is Not a Typical Ja-or Sa-responsive Genementioning

confidence: 99%

Induction of a leaf specific geranylgeranyl pyrophosphate synthase and emission of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene in tomato are dependent on both jasmonic acid and salicylic acid signaling pathways

Ament

Schie

Bouwmeester³

et al. 2006

Planta

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Two cDNAs encoding geranylgeranyl pyrophosphate (GGPP) synthases from tomato (Lycopersicon esculentum) have been cloned and functionally expressed in Escherichia coli. LeGGPS1 was predominantly expressed in leaf tissue and LeGGPS2 in ripening fruit and Xower tissue. LeGGPS1 expression was induced in leaves by spider mite (Tetranychus urticae)-feeding and mechanical wounding in wild type tomato but not in the jasmonic acid (JA)-response mutant def-1 and the salicylic acid (SA)-deWcient transgenic NahG line. Furthermore, LeGGPS1 expression could be induced in leaves of wild type tomato plants by JA-or methyl salicylate (MeSA)-treatment. In contrast, expression of LeGGPS2 was not induced in leaves by spider mite-feeding, wounding, JA-or MeSA-treatment. We show that emission of the GGPP-derived volatile terpenoid (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT) correlates with expression of LeGGPS1. An exception was MeSA-treatment, which resulted in induction of LeGGPS1 but not in emission of TMTT. We show that there is an additional layer of regulation, because geranyllinalool synthase, catalyzing the Wrst dedicated step in TMTT biosynthesis, was induced by JA but not by MeSA.

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Section: Leggps1 Is Not a Typical Ja-or Sa-responsive Genementioning

confidence: 99%

Induction of a leaf specific geranylgeranyl pyrophosphate synthase and emission of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene in tomato are dependent on both jasmonic acid and salicylic acid signaling pathways

Ament

Schie

Bouwmeester³

et al. 2006

Planta

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“…Lycopene accumulation arises during tomato fruit ripening as a consequence of reduced lycopene cyclization (6,7) and the presence of a ripening enhanced phytoene synthase 1 (PSY-1) (6,8). We have shown that the constitutive expression of Psy-1 in transgenic tomato resulted in a number of pleiotrophic effects (9), including dwarfism caused by the diversion of GGPP from gibberellin formation (10).…”

mentioning

confidence: 99%

Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner

Fraser

Römer

Shipton

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

440

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Phytoene synthase from the bacterium Erwinia uredovora (crtB) has been overexpressed in tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig). Fruit-specific expression was achieved by using the tomato polygalacturonase promoter, and the CRTB protein was targeted to the chromoplast by the tomato phytoene synthase-1 transit sequence. Total fruit carotenoids of primary transformants (T 0) were 2-4-fold higher than the controls, whereas phytoene, lycopene, ␤-carotene, and lutein levels were increased 2.4-, 1.8-, and 2.2-fold, respectively. The biosynthetically related isoprenoids, tocopherols plastoquinone and ubiquinone, were unaffected by changes in carotenoid levels. The progeny (T 1 and T2 generations) inherited both the transgene and phenotype. Determination of enzyme activity and Western blot analysis revealed that the CRTB protein was plastid-located and catalytically active, with 5-10-fold elevations in total phytoene synthase activity. Metabolic control analysis suggests that the presence of an additional phytoene synthase reduces the regulatory effect of this step over the carotenoid pathway. The activities of other enzymes in the pathway (isopentenyl diphosphate isomerase, geranylgeranyl diphosphate synthase, and incorporation of isopentenyl diphosphate into phytoene) were not significantly altered by the presence of the bacterial phytoene synthase.carotenoids ͉ metabolic engineering

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“…However, the lycopene content of tomato products is highly variable, being affected by factors such as variety (Hart & Scott, 1995;Abushita et al, 2000), ripeness (Fraser et al, 1994), climate and geographical site of production (Scalfi et al, 2000) and processing (Tonucci et al, 1995;Shi & Le Maguer, 2000;Re et al, 2002). For Body mass index (mean, SE) 412 24.8(0.6) 24.2(0.9) 24.8(0.9) 24.5 (0.8) 26.2 (0.9) 26.2 (0.9) 23.3 (1.0) 25.8 (1.2) 0.4 a TCP=tomato-containing products.…”

Section: Discussionmentioning

confidence: 99%

Tomato consumption and plasma lycopene concentration in people aged 65 y and over in a British national survey

Re¹,

Mishra

Thane

et al. 2003

Eur J Clin Nutr

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Objective: Assessment of consumption of raw and processed tomatoes, plasma lycopene concentration and their interrelationship in a nationally representative sample of elderly British people. Setting: A 1994-1995 National Diet and Nutrition Survey: a nationwide cross-sectional sample of people aged 65 y and over living in mainland Britain, either in the community ('free-living', n ¼ 1275) or in institutions such as nursing homes (n ¼ 412). Methods: Consumption of raw and processed tomatoes (including those in tomato-containing composite foods) was recorded by a 4-day dietary record for each participant. Plasma lycopene concentration was assayed by HPLC. Sociodemographic and lifestyle information was recorded by trained interviewers. Results: In all, 29% of free-living people and 24% of those living in institutions did not consume any raw or processed tomatoes during the 4-day recording period. Free-living participants were more likely to consume raw tomatoes (26 vs 16%). Plasma lycopene concentration was positively associated with the weight of raw and processed tomatoes consumed, especially with processed tomatoes and their products, alcohol consumption and with plasma total (as well as HDL and non-HDL) cholesterol concentrations. Among free-living participants, increased age, cigarette smoking habit and manual occupational social class were all associated with lower plasma lycopene concentration. Conclusions: In the current population, consumption of raw and processed tomatoes varied by sociodemographic and lifestyle characteristics, and made a significant contribution to plasma lycopene concentration. Determinants of plasma lycopene concentration were age, plasma cholesterol concentration and smoking habit.

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Carotenoid Biosynthesis during Tomato Fruit Development (Evidence for Tissue-Specific Gene Expression)

Cited by 481 publications

References 23 publications

Induction of a leaf specific geranylgeranyl pyrophosphate synthase and emission of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene in tomato are dependent on both jasmonic acid and salicylic acid signaling pathways

Induction of a leaf specific geranylgeranyl pyrophosphate synthase and emission of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene in tomato are dependent on both jasmonic acid and salicylic acid signaling pathways

Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner

Tomato consumption and plasma lycopene concentration in people aged 65 y and over in a British national survey

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