“…In all our work using the 3 mm C 18 column, the mobile phase consisted of acetonitrile, methanol and ethyl acetate (e.g., Azevedo-Meleiro and Kimura and Rodriguez-Amaya, 2002;Kimura et al, 2007;Kobori and Rodriguez-Amaya, 2008;Niizu and Rodriguez-Amaya, 2005;Oliveira and Rodriguez-Amaya, 2007). In most of those employing the Vydac column, acetonitrile, methanol and dichloromethane had been employed (e.g., Dias et al, 2008;Englberger et al, 2006;Hart and Scott, 1995;Marsili and Callahan, 1993;Murkovic et al, 2002). On the other hand, a combination of methanol and methyl tert-butyl ether was used in majority of those that utilized the C 30 column (e.g., Aman et al, 2005;Davey et al, 2009a;Failla et al, 2008a;Chandrika et al, 2006;Chitchumroonchokchai et al, 2004;Giuffrida et al, 2007;Goodner et al, 2001;Lessin et al, 1997;Marx et al, 2000;Matsumoto et al, 2007;Mulokozi et al, 2004;Ornelas-Paz et al, 2008;Rouseff et al, 1996).…”