Carotenoid-derived bioactive metabolites shape plant root architecture to adapt to the rhizospheric environments

Ke, Danping; Guo, Jinggong; Li, Kun; Wang, Yujie; Han, Xiaomeng; Fu, Weiwei; Miao, Yuchen; Jia, Kun-Peng

doi:10.3389/fpls.2022.986414

Cited by 10 publications

(6 citation statements)

References 94 publications

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“…CCD4 is a key regulator of carotenoid turnover in different organs of various plant species [49][50][51][52]. In addition, the catabolic products of CCD4 are important bioactives involved in abiotic stress responses [53,54]. It is thus reasonable that IbCCD4 is regulated by transcription factors involved in stress signal transduction.…”

Section: Ibccd4 May Be Involved In Abiotic Stress Responsesmentioning

confidence: 99%

Integrated metabolic and transcriptional analysis reveals the role of carotenoid cleavage dioxygenase 4 (IbCCD4) in carotenoid accumulation in sweetpotato tuberous roots

Zhang

Dong

et al. 2023

Biotechnol Biofuels

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Background Plant carotenoids are essential for human health, having wide uses in dietary supplements, food colorants, animal feed additives, and cosmetics. With the increasing demand for natural carotenoids, plant carotenoids have gained great interest in both academic and industry research worldwide. Orange-fleshed sweetpotato (Ipomoea batatas) enriched with carotenoids is an ideal feedstock for producing natural carotenoids. However, limited information is available regarding the molecular mechanism responsible for carotenoid metabolism in sweetpotato tuberous roots. Results In this study, metabolic profiling of carotenoids and gene expression analysis were conducted at six tuberous root developmental stages of three sweetpotato varieties with different flesh colors. The correlations between the expression of carotenoid metabolic genes and carotenoid levels suggested that the carotenoid cleavage dioxygenase 4 (IbCCD4) and 9-cis-epoxycarotenoid cleavage dioxygenases 3 (IbNCED3) play important roles in the regulation of carotenoid contents in sweetpotato. Transgenic experiments confirmed that the total carotenoid content decreased in the tuberous roots of IbCCD4-overexpressing sweetpotato. In addition, IbCCD4 may be regulated by two stress-related transcription factors, IbWRKY20 and IbCBF2, implying that the carotenoid accumulation in sweeetpotato is possibly fine-tuned in responses to stress signals. Conclusions A set of key genes were revealed to be responsible for carotenoid accumulation in sweetpotato, with IbCCD4 acts as a crucial player. Our findings provided new insights into carotenoid metabolism in sweetpotato tuberous roots and insinuated IbCCD4 to be a target gene in the development of new sweetpotato varieties with high carotenoid production.

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Section: Ibccd4 May Be Involved In Abiotic Stress Responsesmentioning

confidence: 99%

Integrated metabolic and transcriptional analysis reveals the role of carotenoid cleavage dioxygenase 4 (IbCCD4) in carotenoid accumulation in sweetpotato tuberous roots

Zhang

Dong

et al. 2023

Biotechnol Biofuels

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“…Retinal (vitamin A aldehyde) binding precedes the root clock and predicts sites of lateral root organogenesis. According to Ke et al [ 58 ], retinal could be produced from the oxidative cleavage of β-carotene. However, it lacks experimental evidence.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Main Constituents in Two Varieties of Proso Millet Using GC–MS

Kalinová

Tříska

Hořejší

2023

Foods

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Proso millet (Panicum miliaceum) is neglected in human nutrition. Thanks to the composition of the grains, millet is suitable for people with celiac disease and it is also useful in the prevention of cardiovascular diseases. For screening the substances in all plant parts of millet via GC–MS, two varieties, Hanacká Mana and Unicum, were used. Substances from the group saccharides, amino acids, fatty acids, carboxylic acids, phytosterols and others were identified in the roots, leaves, stems, and seeds. The highest level of saccharides was found in the stems (83%); amino acids in the roots (6.9%); fatty acids in the seeds (24.6%); carboxylic acids in the roots (3%), phytosterols in the seeds (10.51%); other substances, such as tetramethyl-2-hexadecenol (1.84%) and tocopherols (2.15%), in the leaves; retinal in the roots (1.30%) and squalene in the seeds (1.29%). Saccharides were the dominant group in all plant parts of proso millet followed by fatty acids. The dominant saccharides in all parts of the millet plant were sucrose, fructose and psicose. On the contrary, turanose, trehalose, glucose and cellobiose belonged to the least represented sugars. Additionally, amyrin, miliacin, campesterol, stigmasterol, β-sitosterol, and others were identified. Varietal variability can be assumed, e.g., in retinal, miliacin or amyrin content.

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“…This suggests that there may be some correlation between the content of red carotenoids and the development of the leaflet. The red carotenoids may promote growth, or their breakdown products may be signals to stop it, as carotenoids are known precursors of plant hormones [27]. There is no evidence yet that carotenoid cleavage dioxygenases (CCD) can use kappa carotenoids as a substrate [28].…”

Section: Changes In Red and Yellow Carotenoids During Developmentmentioning

confidence: 99%

Changes in the Carotenoids of Zamia dressleri Leaves during Development

Murillo,

Nagy,

Menchaca

et al. 2024

Plants

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It has been observed that the leaves of some Zamia species undergo a kind of “reverse ripening”; that is, they change from their original brown color to green during development. We assumed that this strange color change was due to the change in carotenoid composition, so we followed the changes for several weeks. The detailed carotenoid composition and content at different stages of development of the leaves was determined with HPLC-DAD focusing on the changes in red and yellow carotenoids. The total and relative amounts of red and yellow carotenoids were determined simultaneously from one measurement from a saponified and/or unsaponified extract. At the beginning of development, the concentration of red carotenoids was higher than that of the yellow ones; it decreased drastically until 22 days and continued to decrease slowly until they completely disappeared. The concentration of yellow carotenoids decreased at the beginning as well, but after 22 days it started to increase. The amount of red carotenoids started to decrease when the leaflet stopped growing. Lutein is the main component in old leaflets, which is not a red carotenoid precursor. Red carotenoids can always be found in their esterified form in the leaves. These findings support the hypothesis that red and yellow carotenoid accumulation are independent and probably have different functions in the leaflet. The strange color change was explained based on the compartmentalization of red and yellow carotenoids and on the changing activity of the enzyme capsanthin–capsorubin synthase responsible for the synthesis of red carotenoids capsorubin and capsanthin.

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Carotenoid-derived bioactive metabolites shape plant root architecture to adapt to the rhizospheric environments

Cited by 10 publications

References 94 publications

Integrated metabolic and transcriptional analysis reveals the role of carotenoid cleavage dioxygenase 4 (IbCCD4) in carotenoid accumulation in sweetpotato tuberous roots

Integrated metabolic and transcriptional analysis reveals the role of carotenoid cleavage dioxygenase 4 (IbCCD4) in carotenoid accumulation in sweetpotato tuberous roots

Comparison of the Main Constituents in Two Varieties of Proso Millet Using GC–MS

Changes in the Carotenoids of Zamia dressleri Leaves during Development

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