Carotenoid determination in recent marine sediments - practical problems during sample preparation and HPLC analysis

Krajewska, Magdalena; Szymczak‐Żyła, Małgorzata; Kowalewska, Grażyna

doi:10.5267/j.ccl.2017.4.003

Cited by 8 publications

(5 citation statements)

References 23 publications

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“…The pigment extraction, analysis, and quantification were carried out using the procedures described in detail elsewhere (Krajewska et al, ; Szymczak‐Żyła, ; Szymczak‐Żyła et al, , ). In brief, ultrasound‐assisted extraction of wet sediment samples with acetone was applied.…”

Section: Methodsmentioning

confidence: 99%

Present and Past‐Millennial Eutrophication in the Gulf of Gdańsk (Southern Baltic Sea)

Szymczak‐Żyła

Krajewska

Witak

et al. 2019

Paleoceanog and Paleoclimatol

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Eutrophication is manifested by increased primary production leading to oxygen depletion in near‐bottom water and toxic cyanobacteria blooms. This is an important contemporary problem of the Baltic Sea and many other coastal waters. The present eutrophication is mainly ascribed to anthropogenic activity. To compare the present trophic state with that during past millennia, two sediment cores (50 cm long and ~400 cm long covering ca. 5,500 years) were taken from the Gulf of Gdańsk (southern Baltic Sea). The core subsamples were analyzed for phytoplankton pigments (chlorophylls and their derivatives, and carotenoids). In addition, carbon (Ctot, Corg, and δ13C), 14C dating, grain size, diatoms, and selected metals in the cores were analyzed to determine conditions in the depositional environment. The results indicated that there were high primary production periods in the past, during the Littorina Sea and the Roman Climatic Optimum, accompanied by oxygen deficiency in the near‐bottom water, most probably caused by climate warming. The ratio of 132,173‐cyclopheophorbide‐a enol, a labile degradation product of chlorophyll‐a, to the sum of other chloropigments‐a (CPPB‐aE/ΣChlns‐a) is proposed as a new paleoredox proxy. Heterocystous cyanobacteria blooms of an intensity similar to or even greater than at present also occurred in past millennia and were connected with climate warming. Hence, eutrophication must have occurred in the past, which means that natural factors have a substantial influence on it.

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Section: Methodsmentioning

confidence: 99%

Present and Past‐Millennial Eutrophication in the Gulf of Gdańsk (Southern Baltic Sea)

Szymczak‐Żyła

Krajewska

Witak

et al. 2019

Paleoceanog and Paleoclimatol

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“…For exact analysis, separation of these molecules is necessary. Commonly used methods such as HPLC can discriminate molecules based on their mass and affinity to solvents and stationary phase but require chemical pretreatments, which can impair results, for example, dissolution in solvents 69 . Therefore, nondestructive analytical methods provide great advantages in studying samples in their natural states.…”

Section: Resultsmentioning

confidence: 99%

Fast screening of carotenoids of gypsum endoliths using portable Raman spectrometer (Messinian gypsum, Sicily)

Němečková

Jehlička

Culka

2020

J Raman Spectroscopy

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We tested the potential of a portable Raman spectrometer—RaPort (G) by EnSpectr with a 532‐nm laser for use in field detection of biomarkers of endolithic colonization in gypsum. This study extends our previous research on gypsum endolithic colonies using laboratory Raman microspectrometer (514.5‐ and 785‐nm lasers). In the current study, variously coloured layers were found and screened separately on the outcrop under natural conditions. In the recorded Raman spectra, stretching vibrations of carotenoids were observed—ν1 (C═C) around 1,513 cm−1, and ν2 (C—C) around 1,154 cm−1, as well as the feature at around 1,005 cm−1. The exact identification of different carotenoids by portable Raman spectrometer is limited. Nevertheless, the portable Raman spectrometer allowed fast and in situ detection of carotenoids of variously pigmented gypsum endolithic zones. Application of miniature Raman instruments is important in the context of forthcoming astrobiological missions to Mars (Exomars and Mars 2020).

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“…Zeaxanthin and canthaxanthin were extracted by acetone from either carotenoid-bound proteins or E. coli membranes as described earlier 5,48 . Absorbance spectra of carotenoids in organic solvents were registered on a Nanophotometer NP80 (Implen, Germany) using the following molar extinction coefficients: 121,300 M -1 cm -1 for βCar at 466 nm in DMSO 49 , 90,000 M -1 cm -1 for CAN at 472 nm in ethanol 50 , 125,000 M -1 cm -1 for AXT at 482 nm in DMSO 51 , 145,000 M -1 cm -1 for ZEA at 450 nm in methanol 52 . TRITC-conjugated phalloidin and Hoechst 33342 were purchased from Thermo Fisher (USA).…”

Section: Methodsmentioning

confidence: 99%

Nanocontainer derived from silkworm carotenoprotein for carotenoid extraction and presentation in biotechnology and biomedical applications

Sluchanko

Slonimskiy

Egorkin

et al. 2022

Preprint

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Found in many organisms, soluble carotenoproteins are considered as antioxidant nanocarriers for biomedical applications, although the structural basis for their carotenoid transfer function, a prerequisite for rational bioengineering, is largely unknown. We report crystal structures of the Carotenoid-Binding Protein from Bombyx mori (BmCBP) in apo- and zeaxanthin (ZEA)-bound forms. We use spectroscopy and calorimetry to characterize how ZEA and BmCBP mutually affect each other in the complex, identify key carotenoid-binding residues, confirm their roles by crystallography and carotenoid-binding capacity of BmCBP mutants and reconstitute BmCBP complexes with biomedically-relevant xanthophylls lutein, zeaxanthin, canthaxanthin and astaxanthin. By cost-effectively and scalably solubilizing xanthophylls from various crude herbal extracts, His-tagged BmCBP remains monomeric and forms a dynamic nanocontainer delivering carotenoids to liposomes and to other carotenoid-binding proteins, which in particular makes the Orange Carotenoid Protein, a promising optogenetic tool, photoactive. Furthermore, BmCBP(ZEA) administration stimulates fibroblast growth, which paves the way for its biomedical applications.

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Carotenoid determination in recent marine sediments - practical problems during sample preparation and HPLC analysis

Cited by 8 publications

References 23 publications

Present and Past‐Millennial Eutrophication in the Gulf of Gdańsk (Southern Baltic Sea)

Present and Past‐Millennial Eutrophication in the Gulf of Gdańsk (Southern Baltic Sea)

Fast screening of carotenoids of gypsum endoliths using portable Raman spectrometer (Messinian gypsum, Sicily)

Nanocontainer derived from silkworm carotenoprotein for carotenoid extraction and presentation in biotechnology and biomedical applications

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