Carotenoid levels in human lymphocytes, measured by Raman microspectroscopy

Ramanauskaite, R.; Segers-Nolten, Ine G.M.J.; Grauw, K. J. de; Sijtsema, N.M.; Maas, L. van der; Greve, Jan; Otto, Cornelis; Figdor, Carl G.

doi:10.1351/pac199769102131

Cited by 21 publications

(18 citation statements)

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“…In accord with our observations, high amounts of carotenoids at specific sites in the cytoplasm, e. g. the Gall bodies, of T lymphocytes and NK cells but not in the nucleus [18,19] have been found. In bovine luteal cells b-carotene could be detected throughout the volume of the cell, but was predominantly localized in lipid-rich cell components such as the microsomal fraction [20].…”

Section: Discussionsupporting

confidence: 91%

Visualization of astaxanthin localization in HT29 human colon adenocarcinoma cells by combined confocal resonance Raman and fluorescence microspectroscopy

Briviba¹,

Bornemann

Lemmer

2006

Molecular Nutrition Food Res

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Astaxanthin, a carotenoid found in plants and seafood, exhibits antiproliferative, antioxidant and anticarcinogenic properties. We show that astaxanthin delivered with tetrahydrofuran is effectively taken up by cultured colon adenocarcinoma cells and is localized mostly in the cytoplasm as detected by confocal resonance Raman and broad-band fluorescence microspectroscopy image analysis. Cells incubated with beta-carotene at the same concentration as astaxanthin (10 microM) showed about a 50-fold lower cellular amount of beta-carotene, as detected by HPLC. No detectable Raman signal of beta-carotene was found in cells, but a weak broad-band fluorescence signal of beta-carotene was observed. beta-Carotene, like astaxanthin, was localized mostly in the cytoplasm. The heterogeneity of astaxanthin and beta-carotene cellular distribution in cells of intestinal origin suggests that the possible defense against reactive molecules by carotenoids in these cells may also be heterogeneous.

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Section: Discussionsupporting

confidence: 91%

Visualization of astaxanthin localization in HT29 human colon adenocarcinoma cells by combined confocal resonance Raman and fluorescence microspectroscopy

Briviba¹,

Bornemann

Lemmer

2006

Molecular Nutrition Food Res

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“…121 In the 1990s, WBCs were the subject of further Raman spectroscopic analysis by other groups. It was reported that the level of carotenoids in one type of lymphocyte decreased with donor age, 122 that both reduced and oxidized forms of important enzymes cytochrome b558 and myeloperoxidase (MPO) could be distinguished, 123 and that these important enzymes could be elucidated using Raman spectroscopy (the redox state of both cytochrome b558 and MPO were changed upon cell activation, which demonstrated that processes inside living neutrophils could be monitored using Raman spectroscopy). 124,125 Raman spectroscopic studies of WBCs continued through the first decade of the 2000s and new technologies such as Raman mapping 126 and LTRS 127 were utilized.…”

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confidence: 99%

Raman Spectroscopy of Blood and Blood Components

et al. 2017

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Blood is a bodily fluid that is vital for a number of life functions in animals. To a first approximation, blood is a mildly alkaline aqueous fluid (plasma) in which a large number of free-floating red cells (erythrocytes), white cells (leucocytes), and platelets are suspended. The primary function of blood is to transport oxygen from the lungs to all the cells of the body and move carbon dioxide in the return direction after it is produced by the cells' metabolism. Blood also carries nutrients to the cells and brings waste products to the liver and kidneys. Measured levels of oxygen, nutrients, waste, and electrolytes in blood are often used for clinical assessment of human health. Raman spectroscopy is a nondestructive analytical technique that uses the inelastic scattering of light to provide information on chemical composition, and hence has a potential role in this clinical assessment process. Raman spectroscopic probing of blood components and of whole blood has been on-going for more than four decades and has proven useful in applications ranging from the understanding of hemoglobin oxygenation, to the discrimination of cancerous cells from healthy lymphocytes, and the forensic investigation of crime scenes. In this paper, we review the literature in the field, collate the published Raman spectroscopy studies of erythrocytes, leucocytes, platelets, plasma, and whole blood, and attempt to draw general conclusions on the state of the field.

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“…Research in this area are mainly related to lymphocytes due to the protective role played by carotenoids in the human immune system. In addition to studies devoted to distribution of carotenoids in lymphocytes [7][8][9], the effect of age [45] and health condition [10] of the organism was investigated by Raman spectroscopy and Raman imaging. Furthermore, Raman spectroscopy was used to assign the spectral signature of different type of cells: human colon adenocarcinoma [4], gastric carcinoma [5] and human breast cancer cells [6,46,47].…”

Section: Carotenoids In Single Human Cellsmentioning

confidence: 99%

“…the intracellular carotenoids concentration in lymphocytes decreased significantly both with age [45] and in the case of lung cancer patients [10].…”

Section: Lymphocytesmentioning

confidence: 99%

“…the increased amount of carotenoids in specific places in the cytoplasm (for example gall bodies) was observed in both types of cells. In addition, no traces of carotenoids were found in the nuclei [7][8][9][10]45]. As it was concluded for several other subjects, carotenoids as hydrophobic compounds prefer lipid-rich environment, therefore they accumulate in the cytoplasm containing less water than nuclei [4].…”

Section: Pathogenic Human Cellsmentioning

confidence: 99%

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Structural and Spatial Analysis of Carotenoids in a Single Cell Monitored by Raman Spectroscopy

Kaczor

Pilarczyk

2013

Challenges and Advances in Computational Chemistry and Physics

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Carotenoids are particularly convenient subjects to study by means of Raman spectroscopy due to very high Raman scattering cross-section associated with their chain vibrations. Raman studies of carotenoids in single cells have a variety of applications starting from monitoring of growth and morphogenesis of unicellular algae and ending with differentiation of cancerous versus non-cancerous tissues in humans. Examples illustrating the potential of Raman spectroscopy to investigate carotenoids structure and distribution with a particular impact on studies using chemometric and computational methods as a tool to analyze experimental data are reviewed in this chapter.

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Carotenoid levels in human lymphocytes, measured by Raman microspectroscopy

Abstract: Carotenoid levels in lymphocytes obtained from peripheral blood of healthy

Cited by 21 publications

References 1 publication

Visualization of astaxanthin localization in HT29 human colon adenocarcinoma cells by combined confocal resonance Raman and fluorescence microspectroscopy

Visualization of astaxanthin localization in HT29 human colon adenocarcinoma cells by combined confocal resonance Raman and fluorescence microspectroscopy

Raman Spectroscopy of Blood and Blood Components

Structural and Spatial Analysis of Carotenoids in a Single Cell Monitored by Raman Spectroscopy

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