Carotenoid Overproduction in Microalgae: Biochemical and Genetic Engineering

Scarsini, Matteo; Marchand, Justine; Schoefs, Benoı̂t

doi:10.1007/978-3-030-50971-2_5

Cited by 7 publications

(9 citation statements)

References 234 publications

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“…Therefore, the difference in lifestyle exposed the morphotypes to light variation with the fusiform cells benefiting from a higher exposure. Moreover, it is to note that most of these proteins are involved in the carotenoid biosynthesis pathway (Pds, Psy, Zep, Zds) ( Bertrand, 2010 ; Scarsini et al, 2020 ) and in photosynthesis (Gsat; Schoefs and Bertrand, 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes

et al. 2022

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Phaeodactylum tricornutum is an atypical diatom since it can display three main morphotypes: fusiform, triradiate, and oval. Such pleomorphism is possible thanks to an original metabolism, which is tightly regulated in order to acclimate to environmental conditions. Currently, studies dedicated to the comparison of each morphotype issued from one specific strain are scarce and little information is available regarding the physiological significance of this morphogenesis. In this study, we performed a comparative proteomic analysis of the three morphotypes from P. tricornutum. Cultures highly enriched in one dominant morphotype (fusiform, triradiate, or oval) of P. tricornutum Pt3 strain were used. Pairwise comparisons highlighted biological processes, which are up- and down-regulated in the oval (e.g., purine and cellular amino acid metabolism) and triradiate morphotypes (e.g., oxido-reduction and glycolytic processes) compared to the fusiform one used as a reference. Intersection analysis allowed us to identify the specific features of the oval morphotype. Results from this study confirmed previous transcriptomic RNA sequencing observation showing that the oval cells present a distinct metabolism with specific protein enrichment compared to fusiform and triradiate cells. Finally, the analysis of the secretome of each morphotype was also performed.

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Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes

et al. 2022

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“…Most of these compounds are high value molecules. For instance, the price of the natural carotenoid astaxanthin is over $14,000 US kg −1 (Scarsini et al, 2020). Microalgae can also be used in phytoremediation process or in preparation of element-enriched biomass for nutritional purposes (Babaei et al, 2017) or biofertilizers (Gojkovic et al, 2014).…”

Section: Microalgae and Biotechnologymentioning

confidence: 99%

Plasma Applications in Microalgal Biotechnology

Nguyen

Scarsini

Poncin‐Epaillard

et al. 2022

Agritech: Innovative Agriculture Using Microwaves and Plasmas

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Microalgae are very important organisms for the biosphere because they are at the basis of most of the food chains. Some taxa can also bloom and release dangerous toxins in the environment.They are also very promising for industrial applications in various sectors, including food, feed, pharmaceutical, wellness, energy, building, space, water treatment, biosensing, biotechnology etc. The development of these applications require in many cases the optimization of processing steps.Plasma technology has been already applied on biomolecules, proteins, enzymes, peptides in the biomedical field, for the preparation of bioactive, antifouling surfaces of biomaterials. Nowadays, the research prospective deals with the agriculture domain in the purpose of cleaning, sterilizing or fertilizing the soil. However, plasma technology could also be explored in the case of bioenergetic cells where the plasma chemistry enhances bioadhesion and, bioproduction without destroying the microalgae. This chapter gives illustrations on this new application of plasma technology.

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“…Microalgae have been identified as promising sources for the production of biomolecules for energy, food, feed, health, pharmaceutical, and cosmetic industries [1]. Among the biomolecules extracted from microalgae, carotenoids occupy a historical and prominent place [2] because they exhibit unique properties such as provitamin A, antioxidant and skin protection activities [3]. The powerful antioxidative effect of carotenoids is due to the scavenging of free radicals.…”

Section: Introductionmentioning

confidence: 99%

“…β-carotene is an ubiquitous pigment in microalgae due to its requirement for the photosynthetic process [2]. In a few taxa, such as the green microalga Dunaliella sp., the coupled application of nitrogen depletion and high light stress triggers the reorientation of the carbon metabolism toward the massive accumulation of β-carotene: up to 14% of dry weight (DW) [4].…”

Section: Introductionmentioning

confidence: 99%

“…Most of these steps consume time and energy intensively [6], which jeopardizes the ecology and economic viabilities of these processes unless the prices of this type of biomolecules are very high. The expensive market prices of those active compounds from microalgae ultimately reduce their uses for medical treatment and favour the use of chemically synthetized molecules [2]. To reduce production residues, in situ extraction methods or milking have been proposed.…”

Section: Introductionmentioning

confidence: 99%

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Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from Dunaliella salina

et al. 2021

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Biocompatible extraction emerges recently as a means to reduce costs of biotechnology processing of microalgae. In this frame, this study aimed at determining how specific culture conditions and the associated cell morphology impact the biocompatibility and the extraction yield of β-carotene from the green microalga Dunaliella salina using n-decane. The results highlight the relationship between the cell disruption yield and cell volume, the circularity and the relative abundance of naturally permeabilized cells. The disruption rate increased with both the cell volume and circularity. This was particularly obvious for volume and circularity exceeding 1500 µm3 and 0.7, respectively. The extraction of β-carotene was the most biocompatible with small (600 µm3) and circular cells (0.7) stressed in photobioreactor (30% of carotenoids recovery with 15% cell disruption). The naturally permeabilized cells were disrupted first; the remaining cells seems to follow a gradual permeabilization process: reversibility (up to 20 s) then irreversibility and cell disruption. This opens new carotenoid production schemes based on growing robust β-carotene enriched cells to ensure biocompatible extraction.

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Carotenoid Overproduction in Microalgae: Biochemical and Genetic Engineering

Cited by 7 publications

References 234 publications

Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes

Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes

Plasma Applications in Microalgal Biotechnology

Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from Dunaliella salina

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