Carotenoid-Producing Yeasts in the Brazilian Biodiversity: Isolation, Identification and Cultivation in Agroindustrial Waste

Otero, Deborah Murowaniecki; Bulsing, Bruna A.; Huerta, Katira da Mota; Rosa, Carlos A.; Zambiazi, Rui Carlos; Burkert, Carlos André Veiga; Burkert, Janaína Fernandes de Medeiros

doi:10.1590/0104-6632.20190361s20170433

Cited by 21 publications

(8 citation statements)

References 40 publications

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“…From this period, the biomass had a slower growth, with oscillations of up to 168 h, reaching a value of 8.0. In the studies by Otero et al [33], with the Rhodotorula genus, a rapid consumption of reducing sugars was verified, 50% reduction in the first 24 hours of cultivation in an agro-industrial coproduct (parboiled rice water and sugarcane molasses). This consumption is due to conversion to biomass that doubled in the first few hours of cultivation.…”

Section: Confirmation Experimentsmentioning

confidence: 91%

Production of microbial carotenoids by Rhodotorula lactosa isolated in the Brazilian tropical savanna / Produção de carotenoides microbianos por Rhodotorula lactosa isolada na savana tropical brasileira

Machado¹,

Bianchi²

2021

BJDV

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Exploration of new biomes to obtain microorganisms of industrial interest can reveal a number of new strains but they are not all equally efficient in their carotenoids production under different environmental conditions. Screening is needed to select the elite strain and thus commercially produce the pigment. The main objective of this study was to increase the carotenoid production capacity of a recently isolated yeast obtained from the Cerrado (Brazilian tropical savanna) biome. Four hundred and seventy yeast colonies were isolated, of which 25 were pigment-producing yeasts. The promising yeast was identified, by molecular analysis (polymerase chain reaction -PCR) as Rhodotorula lactose, presenting 4.0 g/L of biomass and 776.67 µg/L (194.17 µg/g) of carotenoids (volumetric and specific) in the commercial medium. In the optimization, the carbon source (43 g/L of glucose) was the most significant and positive factor in the carotenoid production process (1318.27 µg/L and 145.58 µg/g), followed by the concentration of nitrogen sources (0.7 g/L yeast extract and 0.7 g/L peptone). The equation generated from the optimized process was validated and showed similar values in the carotenoid production (1233.80 µg/L and 156.60 µg/g) proving it to be promising process model for a scale-up using submerged culture.

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Section: Confirmation Experimentsmentioning

confidence: 91%

Production of microbial carotenoids by Rhodotorula lactosa isolated in the Brazilian tropical savanna / Produção de carotenoides microbianos por Rhodotorula lactosa isolada na savana tropical brasileira

Machado¹,

Bianchi²

2021

BJDV

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“…Three yeast strains isolated in the Brazilian forests, belonging to the species Sporidiobolus pararoseus , Rh. mucilaginosa , and Pichia fermentans , were found to produce cryptoxanthin and β-carotene when they were cultivated in the media containing parboiled rice water and crude glycerol or parboiled rice water and sugar cane molasses [ 155 , 156 ].…”

Section: Biotechnologymentioning

confidence: 99%

Carotenoids and Some Other Pigments from Fungi and Yeasts

et al. 2021

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Carotenoids are an essential group of compounds that may be obtained by microbiological synthesis. They are instrumental in various areas of industry, medicine, agriculture, and ecology. The increase of carotenoids’ demand at the global market is now essential. At the moment, the production of natural carotenoids is more expensive than obtaining their synthetic forms, but several new approaches/directions on how to decrease this difference were developed during the last decades. This review briefly describes the information accumulated until now about the beneficial effects of carotenoids on human health protection, their possible application in the treatments of various diseases, and their use in the food and feed industry. This review also describes some issues that are linked with biotechnological production of fungal and yeasts carotenoids, as well as new approaches/directions to make their biotechnological production more efficient.

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“…These include enzymes, antibiotics, and biopigments that can be produced by submerged and/or solid fermentation of nutrientrich wastes [3][4][5]. Studies on the replacement of traditional culture media with agro-industrial wastes demonstrated increments of up to 50% pigment production by fungi [6]. Compared with fungal pigments, most bacterial pigments are still at the research and development stage; thus, it is necessary to intensify the research on bacterial pigment production to make them available on the market [7].…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of potato solid waste into antifungals and biopigments using Streptomyces spp.

et al. 2021

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Potato waste was processed and used as a sole substrate for simultaneously producing antifungals and biopigments using Streptomyces spp. Out of three different Streptomyces isolates, strain SO6 stood out due to its ability to produce antifungals against economically important fungal phytopathogens and intracellular biopigments using potato waste powders without additional nutrients. This strain also showed the potential to secrete a broad range of enzymes for fermentation of eight sugars that could be involved in potato waste bioconversion. The results of the fermentation assay indicated that Streptomyces sp. strain SO6 degrades potato wastes during submerged fermentation, diminishing total dry weight and increasing reducing sugars from 0.3 to 3.6 mg·mL−1 and total proteins from 70.6 to 187.7 μg·mL−1. The results showed that Streptomyces strain SO6 was able to convert the potato waste into 0.96 mg·g−1 of diffusible antifungals and 1.75 mg·g−1 of reddish-purple biopigments. On the contrary, an absence of pigment production was observed during the fermentation of the commercial medium used as reference. According to our results, replacement of commercial culture media with available low-cost agroindustrial wastes for producing bioactive chemicals is a real opportunity to enhance the Streptomyces pigment production and antibiotic sustainability with cost-competitiveness. To our knowledge, this is the first report on the simultaneous production of biopigments and diffusible antifungal antibiotics produced by Streptomyces spp. using potato solid waste as the sole nutrient source.

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Carotenoid-Producing Yeasts in the Brazilian Biodiversity: Isolation, Identification and Cultivation in Agroindustrial Waste

Cited by 21 publications

References 40 publications

Production of microbial carotenoids by Rhodotorula lactosa isolated in the Brazilian tropical savanna / Produção de carotenoides microbianos por Rhodotorula lactosa isolada na savana tropical brasileira

Production of microbial carotenoids by Rhodotorula lactosa isolated in the Brazilian tropical savanna / Produção de carotenoides microbianos por Rhodotorula lactosa isolada na savana tropical brasileira

Carotenoids and Some Other Pigments from Fungi and Yeasts

Bioconversion of potato solid waste into antifungals and biopigments using Streptomyces spp.

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