Carotenoid-Producing Yeasts: Selection of the Best-Performing Strain and the Total Carotenoid Extraction Procedure

Šovljanski, Olja; Saveljić, Anja; Tomić, Ana; Šeregelj, Vanja; Lončar, Biljana; Cvetković, Dragoljub; Ranitović, Aleksandra; Pezo, Lato; Ćetković, Gordana; Markov, Siniša; Čanadanović-Brunet, Jasna

doi:10.3390/pr10091699

Cited by 12 publications

(3 citation statements)

References 51 publications

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“…In order to understand the influence of the yhfS gene on the carotenoid synthesis ability of tested Bt, carotenoid contents were analyzed and compared between LLP29, LLP29 Δ‐ yhfS and LLP29 R‐ yhfS . The extraction of carotenoids was performed with acid extraction according to the method reported previously 31,32 …”

Section: Methodsmentioning

confidence: 99%

Effect of yhfS gene on Bt LLP29 antioxidant and UV ray resistance

Liu

Zhang

et al. 2023

Pest Management Science

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BACKGROUND: Bacillus thuringiensis (Bt) is a widely used microbial insecticide. However, its persistence is limited because of ultraviolet (UV) rays or other environmental factors. The yhfS gene, which encodes acetyl-CoA acyltransferase, plays an important role in lipid transport and metabolism in many organisms. To explore whether it is related to the stress resistance of Bt LLP29, the yhfS gene knockout strain LLP29 Δ-yhfS and the complementary strain LLP29 R-yhfS were generated successfully by homologous recombination technology, and the related phenotypic changes were compared in this study. RESULTS: Gene yhfS was found to be functional in response to UV radiation in Bt by comparing the survival rates of Bt LLP29 harboring yhfS or not under UV light. Enzyme activity assays of key enzymes showed the the Embden-Meyerhof-Parnas pathway was enhanced yet the tricarboxylic acid cycle as well as butanoate synthesis were repressed when the gene was deleted. At the same time, the amino acid content was decreased, but reduced nicotinamide adenine dinucleotide (NADH) and reactive oxygen species (ROS) content were increased. Most noteworthy, antioxidase (such as superoxide dismutase and peroxidase) activities and contents of some potent antioxidants (such as pyruvate, carotenoids and NADPH) were lower in LLP29 Δ-yhfS than in LLP29.CONCLUSION: These tests revealed that the loss of the yhfS gene led to metabolic disorders and reduction of the antioxidant ability of Bt. Higher ROS level and lower anti-oxidative capacity might be responsible for the reduced UV resistance when the gene was deleted. These results not only greatly enrich understanding of the mechanism of Bt UV resistance, but also provide an important theoretical basis for Bt application.

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Section: Methodsmentioning

confidence: 99%

Effect of yhfS gene on Bt LLP29 antioxidant and UV ray resistance

Liu

Zhang

et al. 2023

Pest Management Science

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“…Based on the optimized protocol described by [41], after 15, 18, 21 and 24 h of culture in YPD medium at 30 • C, cells were centrifuged at 6000× g for 10 min, and pellets were lyophilized and weighed. Lyophilized pellets were then dissolved in acetone and broken by 3 cycles of 20 s in a Fast Prep FP120 (Thermofisher, Waltham, MA, USA).…”

Section: Uv Detection Of Carotenoidsmentioning

confidence: 99%

A New Role for Yeast Cells in Health and Nutrition: Antioxidant Power Assessment

Gosselin-Monplaisir

Dagkesamanskaya

Rigal³

et al. 2023

IJMS

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As the use of antioxidant compounds in the domains of health, nutrition and well-being is exponentially rising, there is an urgent need to quantify antioxidant power quickly and easily, ideally within living cells. We developed an Anti Oxidant Power in Yeast (AOPY) assay which allows for the quantitative measurement of the Reactive Oxygen Species (ROS) and free-radical scavenging effects of various molecules in a high-throughput compatible format. Key parameters for Saccharomyces cerevisiae were investigated, and the optimal values were determined for each of them. The cell density in the reaction mixture was fixed at 0.6; the concentration of the fluorescent biosensor (TO) was found to be optimal at 64 µM, and the strongest response was observed for exponentially growing cells. Our optimized procedure allows accurate quantification of the antioxidant effect in yeast of well-known antioxidant molecules: resveratrol, epigallocatechin gallate, quercetin and astaxanthin added in the culture medium. Moreover, using a genetically engineered carotenoid-producing yeast strain, we realized the proof of concept of the usefulness of this new assay to measure the amount of β-carotene directly inside living cells, without the need for cell lysis and purification.

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“…Ghafoor et al [2] compare conventional and green extraction techniques for natural antioxidants from Saudi date fruit, optimizing the supercritical extraction process using a response surface methodology and regression analysis techniques. A team from Serbia [3] explores the carotenoid extraction protocol using yeast fermentation technology, testing various methods for cell lysis, extraction, and solvents.…”

mentioning

confidence: 99%