Carotenoid production by Rhodotorula rubra cultivated in sugarcane juice, molasses, and syrup

Banzatto, David Ariovaldo; Freita, Lidyane Aline de; Mutton, Márcia Justino Rossini

doi:10.1590/s0101-20612013000500003

Cited by 20 publications

(11 citation statements)

References 5 publications

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“…Previous studies on synthesis of carotenoids were conducted on natural substrates as carbon sources, such as grape juice, grape must, peat extract and hydrolyzate, sugar cane juice and molasses, milk whey, corn hydrolyzate (Mata-Gómez et al, 2014). For example, when incubated in molasses medium for 3 days at 30 ° C and stirring rate 200 rpm, the strain R. rubra generated 2.74 mg/L carotenoids (Banzatto et al, 2013). Production of carotenoids by R. glutinis on brewery wastes constituted 0.6 mg/L, 1.2 mg/L and 1.0 mg/L (crude wastewater, glucose enriched wastewater and synthetic sugar medium, respectively).…”

Section: Discussionmentioning

confidence: 99%

Biosynthetic Production of Carotenoids Using Yeast Strains of Genus Rhodotorula on the Cheap Beer Wort Substrate

Besarab¹,

Gerasimovich²,

Кантерова³

et al. 2018

J microb biotech food sci

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Carotenoids having primary significance for animal husbandry, medicine and food industry are objects of studies to optimize their production conditions and to establish their complex role in living systems. Synthesis of carotenoids by microorganisms is preferential for industrial use due to their natural origin and possibility of combining production with waste disposal. Native microbial strains are the basis for selecting superproducers of organic pigments. In this study, the red yeasts of Rhodotorula genus were incubated in beer wort medium at initial cultural parameters (sugar concentration 6 degrees Balling, temperature 26°C, stirring rate 200 rpm, 4 days of fermentation). Analysis of biomass and carotenoid production by Rhodotorula yeast was performed under defined conditions. Two strains of R. glutinis BIM Y-253 and BIM Y-158 synthesizing over 100 µg carotenoids per 1 g biomass, the mark corresponding to the average level of production, were selected. The concentration of carotenoids generated by strain R. glutinis BIM Y-253 in the nutrient medium exceeded 1 mg / l. Screening of red yeast for the types of synthesized carotenoids showed predominance of torulene- and torularhodin-like compounds in isolated total pigment fractions. At the following experimental stage the effect of organic supplements on production of biomass and carotenoids by the selected strain R. glutinis BIM Y-253 was studied. Yeast incubation with 0.05% malic acid – the substrate for final reaction of TCA cycle, and fine grinding of biomass resulted in average carotenoid yield 3,5 mg/l. Our findings demonstrate the potential carotenoid productivity of red yeast at the initial values of incubation parameters, and illustrate the impact of organic compounds on biosynthetic efficiency of native yeast culture.

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Section: Discussionmentioning

confidence: 99%

Biosynthetic Production of Carotenoids Using Yeast Strains of Genus Rhodotorula on the Cheap Beer Wort Substrate

Besarab¹,

Gerasimovich²,

Кантерова³

et al. 2018

J microb biotech food sci

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“…The residual sugarcane molasses was supplied by Usina Japungu Agroindustrial, a sugar and ethanol plant situated in Santa Rita, State of Paraíba, Brazil (6°59'17"S 35°01'24"W). The hydrolysis of molasses sucrose content was performed according to Banzatto et al (2013), what was done before using it in the bioprocess.…”

Section: Sugarcane Molassesmentioning

confidence: 99%

“…Also through an experimental design, Banzatto et al (2013) obtained an optimum dry biomass concentration (14.87 g L -1 ) using sugarcane molasses supplemented with a commercial nutrient named Nitrofosfo KL. The same behavior in relation to the presence of a nitrogen source was observed between the MYEC and MC cultivations, where a yeast extract supplementation (3.0 g L -1 ) increased the microbial biomass content by 15.80%.…”

Section: Rhodotorula Mucilaginosa Cct3892 Growth In Sugarcane Molassesmentioning

confidence: 99%

Oil-lipids, carotenoids and fatty acids simultaneous production by Rhodotorula mucilaginosa CCT3892 using sugarcane molasses as carbon source

Costa

Padilha

Júnior

et al. 2020

Braz. J. Food Technol.

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One of the most important classes of nutritional biomolecules is the oleaginous compounds group, which specially includes the oil-lipids, the carotenoids and the fatty acids. These biocompounds present a wide range of industrial applications because their ability to act as an energy source, antioxidants and metabolic agents for the human body. Therefore, the food industry, mainly focusing on food supplementation, is always searching for new sources of them. In this context, the present study evaluated the total lipids, carotenoids and fatty acids simultaneous production by the Rhodotorula mucilaginosa CCT3892 yeast, using residual sugarcane molasses as carbon source. The results obtained demonstrated that the cultivation of yeast in molasses medium (MC) produced the same content of total lipids and carotenoids (16.50% ± 0.68% and 0.053 ± 0.001 mg g-1, respectively) as the obtained from a synthetic medium (SC) (15.36% ± 1.36% and 0.051 ± 0.001 mg g-1 0.005). Concerning the fatty acids biosynthesis, the MC cultivation generated the most interesting profile once it presented a greater content of oleic acid (74.05%), an unsaturated compound with high nutritional value. The cultivation carried out with the molasses and yeast extract supplementation (MYEC) did not provide an improvement in microbial oil production, what indicated that in this condition there was a predominance of others sorts of substrate metabolization by the yeast cells, as confirmed by the microbial kinetics study.

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“…The agroindustrial byproducts can increase the process economic viability for being a source of nutrients like nitrogen, carbon, and minerals. Studies report the efficient use and viable economic point of view of substrates and their derivate such as glycerol [10,11], sugar cane [12][13][14], corn [15][16][17], among others. Fed-batch processes are reportedly effective and versatile in the most fermentation processes, including the carotenoid production.…”

Section: Introductionmentioning

confidence: 99%

Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

2020

Biointerface Res Appl Chem

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This work aim the carotenoid bioproduction by the yeast Phaffia rhodozyma Y-17268 in a fed-batch bioreactor with different low-cost agroindustrial substrates (crude glycerol, corn steep liquor, and rice parboiling water). The maximum concentration of total carotenoid and cell productivity were 4118 µg/L (835 µg/g) and 0.05 g/L. h, respectively, with a feed volume of 75 mL every 12 h. The medium were composed of 100 g/L crude glycerol, 100 g/L corn steep liquor, and 20 g/L rice parboiling water at 25ºC, pHinitial 4.0, agitation rate of 250 rpm, aeration rate of 1.5 vvm and 96 h of bioproduction. 0.188 h-1 of maximum specific growth speeds (μmax) was obtained for the major carotenoid - (all-E)-β-carotene (75.9%). Thus, the yeast P. rhodozyma produced in a fed-batch bioreactor demonstrated a great potential to produce the β-carotene.

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Carotenoid production by Rhodotorula rubra cultivated in sugarcane juice, molasses, and syrup

Cited by 20 publications

References 5 publications

Biosynthetic Production of Carotenoids Using Yeast Strains of Genus Rhodotorula on the Cheap Beer Wort Substrate

Biosynthetic Production of Carotenoids Using Yeast Strains of Genus Rhodotorula on the Cheap Beer Wort Substrate

Oil-lipids, carotenoids and fatty acids simultaneous production by Rhodotorula mucilaginosa CCT3892 using sugarcane molasses as carbon source

Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

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