Carotenoids located in human lymphocyte subpopulations and natural killer cells by Raman microspectroscopy

Puppels, Gerwin J.; Garritsen, Henk; Kummer, J. Alain; Greve, Jan

doi:10.1002/cyto.990140303

Cited by 96 publications

(65 citation statements)

References 26 publications

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“…Puppels et al used resonance Raman to quantify the amount of carotenoids in different sub-cellular regions [199]. This led to other studies using resonance Raman to create sub-cellular maps of specific molecules including heme-containing enzymes such as cytochrome b 558 [146] and globins such as hemoglobin and neuroglobin [200].…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

270

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

270

189

View full text Add to dashboard Cite

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“…Raman images were previously obtained from lymphocytes using preresonant Raman scattering from carotenoids 3 and red blood cells 4 by utilizing the resonant Raman scattering from hemoglobin. In addition, the lipid and cholesterol distributions inside the lens fiber cells were studied by using Raman microspectroscopy and imaging.…”

Section: Introductionmentioning

confidence: 99%

Nonresonant Raman imaging of protein distribution in single human cells

et al. 2002

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A confocal Raman microscope is used to study the protein distribution inside biological cells. It is shown that high quality Raman imaging of the protein distribution can be obtained using confocal nonresonant Raman imaging ( exc ϭ 647.1 nm). The results are shown for two different human cell types. Perpheral blood lymphocytes are used as an example of the fully maturated cells with a low level of nuclear transcription. Human eye lens epithelial cells are used as an example of cells with a high level of nuclear activity. The protein distribution in both cell types is completely different. The nuclear distribution of the protein largely varies in the peripheral blood lymphocyte cells, while proteins are more homogenously distributed over the nuclear space in the eye lens epithelial cells. The imaging time is ϳ20 min for a field of view of 10 ϫ 10 m 2 . The size of the sampling volume is 1.4 fL using a full width at halfmaximum criterion along the z axis and a 1/e 2 criterion in the xy plane. The results presented here indicate that Raman imaging is particularly of interest in the study of cellular processes, like phagocytosis, apoptosis, chromatin compaction, and cellular differentiation, which are accompanied by relatively large-scale redistributions of the materials.

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“…In 1990, Puppels et al developed a confocal Raman microscope, capable of recording Raman spectra of single human cells and polytene chromosomes (42). Since then, many biological phenomena in single human cells have been studied by Puppel's group; e.g., Raman spectra of the cell nucleus and the cell cytoplasm in human white blood cells were obtained (43)(44)(45)(46). Various groups have reported the classification of bacteria by means of Raman spectroscopy (3,11,12,17,27,(31)(32)(33)(34)47).…”

mentioning

confidence: 99%

Chemotaxonomic Identification of Single Bacteria by Micro-Raman Spectroscopy: Application to Clean-Room-Relevant Biological Contaminations

Rösch

Harz

Schmitt

et al. 2005

Appl Environ Microbiol

260

194

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Microorganisms, such as bacteria, which might be present as contamination inside an industrial food or pharmaceutical clean room process need to be identified on short time scales in order to minimize possible health hazards as well as production downtimes causing financial deficits. Here we describe the first results of single-particle micro-Raman measurements in combination with a classification method, the so-called support vector machine technique, allowing for a fast, reliable, and nondestructive online identification method for single bacteria.

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Carotenoids located in human lymphocyte subpopulations and natural killer cells by Raman microspectroscopy

Cited by 96 publications

References 26 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

Nonresonant Raman imaging of protein distribution in single human cells

Chemotaxonomic Identification of Single Bacteria by Micro-Raman Spectroscopy: Application to Clean-Room-Relevant Biological Contaminations

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