Carotid Artery Stenosis is Exacerbated in Spontaneously Obese Model Rats with Diabetes

Wajima, Daisuke; Nakagawa, Ichiro; Takamura, Yoshiaki; Aketa, Shuta; Yonezawa, Taiji; Nakase, Hiroyuki

doi:10.5551/jat.24661

Cited by 4 publications

(6 citation statements)

References 16 publications

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“…Diabetes causes vascular endothelial dysfunction and hypertrophic remodeling of the carotid artery. The alterations include vessel narrowing, thickening of the tunica media with the deposition of collagen and laminin in the vessel walls, resulting in an accelerate formation of atherosclerosis (8,35,36). In the present report, we observed both a significant increase in common carotid artery wall thickness (tunica intima + tunica media) and some Doppler parameter changes in STZ-induced diabetic rats.…”

Section: Discussionsupporting

confidence: 61%

“…In the present report, we observed both a significant increase in common carotid artery wall thickness (tunica intima + tunica media) and some Doppler parameter changes in STZ-induced diabetic rats. The measurements in our report represent total intima-media thickness and include possible hyperechoic plaques, thus accounting for an increase in size compared to other US studies performed on diabetic rats (37)(38)(39). An increase in the MDA level and a reduction of the GSH level and SOD activity in the carotid artery of diabetic rats were evidenced.…”

Section: Discussionmentioning

confidence: 65%

“…Polyphenols represent a large group of bioactive plant compounds known for their many beneficial effects on the body: antioxidant, antiatherogenic, hypoglycemic, hypocholesterolemic, hypotriglyceridemic, anti-inflammatory, antimicrobial, etc. (8). The flavonoid quercetin is one of the most important natural antioxidants found in many vegetables and fruits (9).…”

Section: Introductionmentioning

confidence: 99%

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Vascular impact of quercetin administration in association with moderate exercise training in experimental type 1 diabetes

Chis

Socaciu

Moldovan

et al. 2019

Revista Romana De Medicina De Laborator

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Hyperglycemia and oxidative stress have a major role in the pathogenesis of diabetic vascular complications. In this study, we investigated the efficacy of combining quercetin treatment with moderate exercise training in reversing diabetes-induced oxidative stress and ultrasound modifications in rat carotid arteries. The diabetic Wistar rats were divided into sedentary groups and trained groups. The trained animals went through a regular moderate exercise by swimming (5 weeks). Some non-diabetic and diabetic rats were daily treated with quercetin (30 mg/kg, for 5 weeks). At the end of the study, the imaging evaluation required to assess the effects of diabetes on carotid arteries was performed by micro-ultrasound (MU). The diabetic rats presented atherosclerotic plaques, with an increase in the echogenicity of the carotid artery wall, carotid intima-media thickness (CIMT), and carotid wall thickness, while the diabetic trained rats treated with quercetin presented normal values of these parameters. Malondialde-hyde (MDA) levels, superoxide dismutase (SOD) antioxidant enzyme activity, reduced glutathione (GSH) levels and the reduced (GSH) to oxidized (GSSG) glutathione ratio were determined in the carotid artery tissue. Diabetes caused elevated MDA levels and a decrease in SOD activity, GSH levels and GSH/GSSG ratio in the carotid artery tissue. Treating diabetic rats with quercetin combined with moderate exercise training reversed all these oxidative stress parameters. Our results show that this combination, quercetin and moderate exercise training, can be a good treatment strategy for the vascular complications of diabetes by attenuating hyperglycemia-mediated oxidative stress.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Vascular impact of quercetin administration in association with moderate exercise training in experimental type 1 diabetes

Chis

Socaciu

Moldovan

et al. 2019

Revista Romana De Medicina De Laborator

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“…Owing to hyperglycemia in diabetes, the delayed repair of vascular endothelial cells (Ecs) increases the risk of VSMc migration to the intima (13). In addition to high glucose toxicity, carotid artery stenosis is exacerbated in diabetic rats with higher levels of FFAs (14). Oleic acid (OA) is one of the most common FFAs in the blood of patients with diabetes, and a previous study demonstrated that excessive OA has an effect on vascular restenosis (15).…”

Section: Introductionmentioning

confidence: 99%

Upregulated 14‑3‑3β aggravates restenosis by promoting cell migration following vascular injury in diabetic rats with elevated levels of free fatty acids

et al. 2018

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Mono‑unsaturated free fatty acids (FFAs) can serve as a predictive indicator of vascular restenosis following interventional therapy, particularly in individuals with high‑fat diet‑induced type 2 diabetes. However, the pathogenic mechanism remains to be fully elucidated. In the present study, the levels of tyrosine 3‑monooxygenase/tryptophan 5‑monooxygenase activation protein β (YWHAB; also known as 14‑3‑3β), in vascular smooth muscle cells (VSMCs) treated with different concentrations of oleic acid (OA) were examined by reverse transcription‑quantitative polymerase chain reaction and western blot analyses. The migration of VSMCs was examined using wound‑healing and Transwell migration assays. The protein distribution of B‑cell lymphoma 2 (BCL‑2)‑associated death promoter (BAD) in VSMCs treated with OA was examined by immunofluorescence and western blot analyses. In in vivo experiments, the carotid artery morphology of rats in different groups was assessed at 14 days post‑injury by non-invasive ultrasonographic imaging and confirmed by histological staining. The expression of YWHAB was upregulated by OA in a concentration‑dependent manner in VSMCs. In the in vivo experiments, carotid stenosis was more serious among high‑FFA diabetic rats. However, silencing of YWHAB significantly alleviated carotid neointimal hyperplasia among the diabetic rats with elevated FFA levels. In addition, YWHAB silencing alleviated the migration of OA‑treated VSMCs and increased translocation of the BAD protein from the cytoplasm to the mitochondria. In conclusion, the results showed that FFA‑induced upregulation of YWHAB was involved in neointimal hyperplasia by enhancing the migration of VSMCs following carotid artery injury. The inhibition of YWHAB may serve as a novel potential pharmacological target for preventing vascular restenosis following interventional therapy in diabetic individuals with high FFA levels.

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“…CCA in rat is an excellent model for studying hypertension 25, 26 , stenosis 26 , and stroke 27 . Especially, CCA is the predilection for the development of atherosclerotic plaques, and low WSS values contribute to the initiation and progression of atherosclerotic disease 28 .…”

Section: Introductionmentioning

confidence: 99%

Optimized analysis of blood flow and wall shear stress in the common carotid artery of rat model by phase-contrast MRI

Peng

Shih

Huang

et al. 2017

Sci Rep

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The present study systemically investigated the influence of gated/non-gated sequences, velocity encoding (VENC), and spatial resolution on blood flow, wall shear stress (WSS), and artery area evaluations when scanning the common carotid artery (CCA) in rats using phase-contrast magnetic resonance imaging (PC-MRI). We first tested whether or not non-gated PC-MRI was appropriate for evaluating blood flow and WSS in rats. For both gated and non-gated techniques, VENC values in the range of 60-120 cm/s with an interval of 10 cm/s were also tested. Second, we optimized the inplane resolution of PC-MRI for blood flow and WSS measurements. Results showed the usage of a gated instrument can provide more reproducible assessments, whereas VENC had an insignificant influence on all hemodynamic measurements (all P > 0.05). Lower resolutions, such as 0.63 mm, led to significant overestimations in blood flow and artery area quantifications and to an underestimation in WSS measurements (all P < 0.05). However, a higher resolution of 0.16 mm slightly increased measurement variation. As a tradeoff between accuracy and scan time, we propose a gated PC-MRI sequence with a VENC of 120 cm/s and a resolution of 0.21 mm to be used to extract hemodynamic information about rat CCA.Evaluating local flow profiles in vessels is a useful means to assess cardiovascular function and physiopathological conditions 1, 2 . It is expected to gain increased popularity in both clinical routine and pre-clinical studies. Doppler ultrasound (US) and phase-contrast (PC) MRI are the methods of choice for measuring flow-related parameters. Doppler US has the advantage of better temporal resolution to capture the peak systolic blood flow velocity. However, US measurement requires considerable technical ability, and it is therefore susceptible to the operator dependency 3 . By using phase shifts in moving spins to quantify the velocity information in flowing vessels, PC-MRI enables the noninvasive assessment of blood rheology 4, 5 . Because it is user-independent and allows straightforward flow quantification, PC-MRI has had an immediate impact on various fields.The major use of PC-MRI is to provide insight into the hemodynamic characteristics of blood flow in large vessels, such as cerebral vessels and carotid arteries. By measuring and integrating flow volume from the main feeding arteries of the brain, e.g., the left/right internal carotid arteries (ICA) and the vertebral artery (VA), one can quantify whole brain cerebral blood flow (CBF) 6, 7 . Wall shear stress (WSS) is the frictional force exerted on the endothelium of vessel walls by the circulation. Prior studies have shown that common carotid arteries (CCA) with a relatively low WSS are prone to developing atherosclerosis 8 and are associated with other cerebrovascular diseases 9 . PC-MRI covers a wide range of significant applications and the derived parameters allow comparisons between normal and pathological blood flow. Optimizing the scanning parameters for PC-MRI is therefore nontrivial, and ...

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Carotid Artery Stenosis is Exacerbated in Spontaneously Obese Model Rats with Diabetes

Cited by 4 publications

References 16 publications

Vascular impact of quercetin administration in association with moderate exercise training in experimental type 1 diabetes

Vascular impact of quercetin administration in association with moderate exercise training in experimental type 1 diabetes

Upregulated 14‑3‑3β aggravates restenosis by promoting cell migration following vascular injury in diabetic rats with elevated levels of free fatty acids

Optimized analysis of blood flow and wall shear stress in the common carotid artery of rat model by phase-contrast MRI

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