Carp growth hormone: molecular cloning and sequencing of cDNA

Koren, Yoram; Sarid, S; Ber, Rosalie; Daniel, Violet

doi:10.1016/0378-1119(89)90078-4

Cited by 55 publications

(21 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gosline (1971), pointed out the affinity of this group with members of the former perciform Suborder Mugiloidei, which is currently in the Mugilomorpha Series, Mugiliformes Order (Stiassny, 1990). This author proposed that atherinomorphs and mugiloids are sis-GH phylogeny of euteleostean fishes 297 , S. aurata (Funkenstein et al, 1991), S. schlegeli (Genbank accession AAB49492), P. olivaceus (Momota et al, 1988), V. variegatus (Genbank accession AAC36716), S. senegalensis (Genbank accession AAA60372), F. rubripes (Venkatesh and Brenner, 1997), G. morhua (Rand-Weaver et al, 1991), O. mykiss (Rentier-Delrue et al, 1989), S. salar (Johansen et al, 1989), C. carpio (Koren et al, 1989), H. nobilis (Bernardi et al, 1993), I. punctatus (Genbank accession AAC60745) and A. japonica (Saito et al 1988).…”

Section: Resultsmentioning

confidence: 99%

A growth hormone-based phylogenetic analysis of euteleostean fishes including a representative species of the Atheriniformes Order, Odontesthes argentinensis

et al. 2003

View full text Add to dashboard Cite

The GH (growth hormone) cDNA sequence of the marine silverside fish Odontesthes argentinensis was obtained using the RACE protocol (Rapid Amplification of cDNA Ends). The marine silverside GH cDNA sequence is 928 nucleotides long and was found to encode a polypeptide of 204 amino acids, including a signal peptide of 17 amino acids. The 5' and 3' untranslated regions of the messenger are 109 and 204 nucleotides long, respectively. The deduced GH amino acid sequence was used to infer a phylogenetic tree with GH amino acid sequences from representative species belonging to the Euteleostei Subdivision using the maximum parsimony method. The topology found is according to the major phylogenetic grouping of euteleosts. The results corroborate the hypothesis that atherinids are not related to paracanthopterygians as previously suggested, and show a lack of solid synapomorphies among most of the Acanthopterygii Orders analysed indicating a complex assemblage of fishes in which the phylogenetic tree remains indeterminable.

show abstract

Section: Resultsmentioning

confidence: 99%

A growth hormone-based phylogenetic analysis of euteleostean fishes including a representative species of the Atheriniformes Order, Odontesthes argentinensis

et al. 2003

View full text Add to dashboard Cite

show abstract

“…The characterization of cGH cDNA isolated from a carp pituitary cDNA library in X-gtll has been described previously (Koren et al 1989). Construc-tion of cGH expression plasmid pO.L-2-FGH, expression of cGH in E. coli and purification of the cGH to over 95% homogeneity is described by Fine et al (1993).…”

Section: Preparation Of Recombinant Cgh and Its C123a Mutantmentioning

confidence: 99%

“…Carp growth hormone (cGH) has also been cloned (Koren et al 1989;Chao et al 1987) and, more recently, expressed in E. coli as an insoluble aggregate (refractile bodies) (Fine et al 1993). Expression in E. coli as an insoluble aggregate is commercially preferable because of high yield, simpler and cheaper purification procedures and resistance to intracellular bacterial proteases (Sugimoto et al 1991b).…”

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Preparation and comparison of biological properties of recombinant carp (Cyprinus carpio) growth hormone and its Cys-123 to Ala mutant

Fine

Sakal

Vashdi

et al. 1993

Fish Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Carp growth hormone (cGH) cDNA, in which Cys-123 was mutated to Ala, was prepared, transferred to the expression vector, expressed in Escherichia coli and the mutant was purified to homogeneity. The mutation only slightly improved yield of the monomeric fraction, indicating that Cys-123 is not involved in improper refolding. As compared to cGH, the mutant (cGH-C123A) exhibited lower binding affinity toward homologous liver receptors and lower bioactivity in a 3T3-F442A preadipocyte bioassay despite the fact that both hormones exhibited almost identical cross-reactivity with anti-cGH antibodies. These results, along with those of a structural comparison to hGH, suggest that Cys-123 is located in the hydrophobic core of the hormone, and is most likely affecting the conformation of the binding site. Dimeric forms of the hormone and its mutant were less active than their respective monomers. Homologous binding experiments using a carp liver microsomal fraction revealed a single receptor population with Kd = 0.77 nM and Bmax = 241 fmol/mg microsomal protein.

show abstract

“…In fishes, GH is also involved in different physiological processes, such as reproduction (Trudeau, 1997) and osmoregulation (Sakamoto et al, 1997). Genomic GH sequences and cDNAs from fishes have been characterized and used as a phylogenetic marker for different taxonomic groups (Koren et al, 1989;Chang et al, 1992;Lemaire et al, 1994;Marins et al, 2003;Clements et. al., 2004).…”

mentioning

confidence: 99%

[No Title Available]

et al. 2008

View full text Add to dashboard Cite

RT-PCR was used for amplifying Piaractus mesopotamicus growth hormone (GH) cDNA obtained from mRNA extracted from pituitary cells. The amplified fragment was cloned and the complete cDNA sequence was determined. The cloned cDNA encompassed a sequence of 543 nucleotides that encoded a polypeptide of 178 amino acids corresponding to mature P. mesopotamicus GH. Comparison with other GH sequences showed a gap of 10 amino acids localized in the N terminus of the putative polypeptide of P. mesopotamicus. This same gap was also observed in other members of the family. Neighbor-joining tree analysis with GH sequences from fishes belonging to different taxonomic groups placed the P. mesopotamicus GH within the Otophysi group. To our knowledge, this is the first GH sequence of a Neotropical characiform fish deposited in GenBank.

show abstract

Carp growth hormone: molecular cloning and sequencing of cDNA

Cited by 55 publications

References 14 publications

A growth hormone-based phylogenetic analysis of euteleostean fishes including a representative species of the Atheriniformes Order, Odontesthes argentinensis

A growth hormone-based phylogenetic analysis of euteleostean fishes including a representative species of the Atheriniformes Order, Odontesthes argentinensis

Preparation and comparison of biological properties of recombinant carp (Cyprinus carpio) growth hormone and its Cys-123 to Ala mutant

[No Title Available]

Contact Info

Product

Resources

About