Carpobrotus edulis (L.) N.E.Br. extract as a skin preserving agent: From traditional medicine to scientific validation

Bazzicalupo, Miriam; Cornara, Laura; Burlando, Bruno; Cascini, Alberta; Denaro, Marcella; Smeriglio, Antonella; Trombetta, Domenico

doi:10.1016/j.joim.2021.09.002

Cited by 14 publications

(19 citation statements)

References 53 publications

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“…The effects of extracts on cell viability were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, while the healing activity with scratch wound assay was evaluated as previously described [39]. Both HaCat and L929 cells were grown in culture flasks at 37 • C, in 5% CO 2 , humidified atmosphere, in Dulbecco's modified Eagle medium enriched with 10% (v/v) fetal bovine serum (EuroClone, Milan, Italy), 1% L-glutamine, and 1% antibiotic mixture (Penicillin/Streptomycin).…”

Section: Cell Viability and Wound Healing Assaysmentioning

confidence: 99%

Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch

et al. 2022

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Peucedanum ostruthium (L.) W. D. J. Koch (Apiaceae) is a worldwide perennial herb native to the mountains of central Southern Europe. The rhizome has a long tradition in popular medicine, while ethnobotanical surveys have revealed local uses of leaves for superficial injuries. To experimentally validate these uses, plant material was collected in the Gran Paradiso National Park, Aosta Valley, Italy, and the rhizome and leaves were micromorphologically and phytochemically characterized. Polyphenol-enriched hydroalcoholic rhizome and leaf extracts, used in cell-free assays, showed strong and concentration-dependent antioxidant and anti-inflammatory activities. In vitro tests revealed cyclooxygenase and lipoxygenase inhibition by the leaf extract, while the rhizome extract induced only lipoxygenase inhibition. MTT assays on HaCaT keratinocytes and L929 fibroblasts showed low cytotoxicity of extracts. In vitro scratch wound test on HaCaT resulted in a strong induction of wound closure with the leaf extract, while the effect of the rhizome extract was lower. The same test on L929 cells showed similar wound closure induction with both extracts. The results confirmed the traditional medicinal uses of the rhizome as an anti-inflammatory and wound healing remedy for superficial injuries but also highlighted that the leaves can be exploited for these purposes with equal or superior effectiveness.

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Section: Cell Viability and Wound Healing Assaysmentioning

confidence: 99%

Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch

et al. 2022

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“…The ORAC assay was carried out according to Bazzicalupo et al [ 32 ]. Briefly, 20 µL of AGE methanol solution (0.031–0.25 μg/mL) and oregonin (0.03–0.20 μg/mL), were added to 120 μL of fresh 117 nM fluorescein and were incubated for 15 min at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

New Insights on Phytochemical Features and Biological Properties of Alnus glutinosa Stem Bark

Smeriglio

D’Angelo

Cacciola

et al. 2022

Plants

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Alnus glutinosa (namely black alder or European alder) is a tree of the Betulaceae family widely spread through Europe, Southeastern Asia, the Caucasus mountains, and Western Siberia. Its bark is traditionally used for medicinal purposes as an astringent, cathartic, febrifuge, emetic, hemostatic, and tonic, suggesting that it may contain bioactive compounds useful to counteract inflammation. The aim of this study was to investigate the phytochemical profile of A. glutinosa stem bark extract (AGE) by LC-DAD-ESI-MS/MS analysis and to validate some biological activities such as antioxidant, anti-inflammatory and anti-angiogenic properties by in vitro and in vivo models (chick chorioallantoic membranes and zebrafish embryos), that can justify its use against inflammatory-based diseases. The AGE showed a high total phenols content expressed as gallic acid equivalents (0.71 g GAE/g of AGE). Diarylheptanoids have been identified as the predominant compounds (0.65 g/g of AGE) with oregonin, which alone constitutes 74.67% of the AGE. The AGE showed a strong and concentration-dependent antioxidant (IC50 0.15–12.21 µg/mL) and anti-inflammatory (IC50 5.47–12.97 µg/mL) activity. Furthermore, it showed promising anti-angiogenic activity, inhibiting both the vessel growth (IC50 23.39 µg/egg) and the release of an endogenous phosphatase alkaline enzyme (IC50 44.24 µg/embryo). In conclusion, AGE is a promising source of antioxidant, anti-inflammatory and angio-modulator compounds.

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“…Total phenols were quantified according to Bazzicalupo et al [ 77 ] using the Folin–Ciocalteu reagent, by mixing each extract solution (6.0–24.0 mg/mL for PCF, RAF, RAC, CIF, CIC, ADF and ADC; 0.5–2.0 mg/mL for PCC) with deionized water and Folin–Ciocalteu reagent in the following ratio: 1:9:10, v / v / v . After 3 min, 10% sodium carbonate (1:2, v / v ) was added and samples were incubated for 60 min in the dark at RT, mixing every 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…The carbohydrates content was determined according to Bazzicalupo et al [ 77 ]. Briefly, 10 mg of fresh and blanched PC, RA, CI and AD were placed in a glass test tube with screw cap, together with 0.5 mL of 2.5 mol/L HCl, and the mixture was incubated in a water bath at 100 °C for 3 h. After cooling, samples were neutralized with sodium carbonate and brought up to 10 mL with distilled water.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Ingredients Incorporated in the Traditional Mixed-Salad of the Capuchin Monks

Cornara

Ambu

Alberto

et al. 2022

Plants

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Recipes on the composition of the “salad of the monks” (Capuchin monks) have been reported in Italy since the 17th century. Different wild edible plants were highly regarded as an important ingredient of this mixed salad. Among these, some species played a key role for both their taste and nutritional properties: Plantago coronopus L. (PC), Rumex acetosa L., Cichorium intybus L., and Artemisia dracunculus L. In the present study, the micromorphological and phytochemical features as well as the antioxidant and anti-inflammatory properties of extracts of these fresh and blanched leaves, were investigated. The extracts obtained by blanched leaves, according to the traditionally used cooking method, showed the highest content of bioactive compounds (total phenols 1202.31–10,751.88 mg GAE/100 g DW; flavonoids 2921.38–61,141.83 mg QE/100 g DW; flavanols 17.47–685.52 mg CE/100 g DW; proanthocyanidins 2.83–16.33 mg CyE/100 g DW; total chlorophyll 0.84–1.09 mg/g FW; carbohydrates 0.14–1.92 g/100 g FW) and possess the most marked antioxidant (IC50 0.30–425.20 µg/mL) and anti-inflammatory activity (IC50 240.20–970.02 µg/mL). Considering this, our results indicate that increased consumption of the investigated plants, in particular of PC, raw or cooked briefly, could provide a healthy food source in the modern diet by the recovery and enhancement of ancient ingredients.

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Carpobrotus edulis (L.) N.E.Br. extract as a skin preserving agent: From traditional medicine to scientific validation

Cited by 14 publications

References 53 publications

Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch

Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch

New Insights on Phytochemical Features and Biological Properties of Alnus glutinosa Stem Bark

Characterization of Ingredients Incorporated in the Traditional Mixed-Salad of the Capuchin Monks

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