Carrageenans in the cell walls of Chondrus crispus Stack. (Rhodophyceae, Gigartinales). I. Localization with fluorescent antibody

Gordon-Mills, Elizabeth; McCandless, Esther L.

doi:10.2216/i0031-8884-14-4-275.1

Cited by 30 publications

(16 citation statements)

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“…K-and A-carrageenans occurring in the cell walls of the appropriate stages of the life cycle were identified by fluorescent antibody technique (GORDON-MILLS and MCCANDLESS 1975). Mild physical separation of the cell walls of Porphyra umbilicalis (GUNAWARDENA and WILLIAMSON 1975) located the agarlike polysaccharide in the intercellular matrix.…”

Section: Cell Wall Localizationmentioning

confidence: 99%

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Algal Walls — Composition and Biosynthesis

Percival¹,

McDowell²

1981

Plant Carbohydrates II

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Section: Cell Wall Localizationmentioning

confidence: 99%

“…She prepared antibodies from the serum of rabbits sensitized by injection of alginate, coupled this with a fluorescent dye and applied it to algal sections for microscopic examination. GORDON and MCCANDLESS (1973) applied this method to the detection of Kcarrageenan and GORDON-MILLS and MCCANDLESS (1975) to A-carrageenan.…”

Section: Introductionmentioning

confidence: 99%

Algal Walls — Composition and Biosynthesis

Percival¹,

McDowell²

1981

Plant Carbohydrates II

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“…The chemical composition of both the KCl-insoluble and soluble polymers did not vary in any systematic manner with photon irradiance or segment of the thallus from which they were isolated . The lambda carrageenan reported from female C. crispus may have originated from the carposporophytes, known to contain lambda carrageenan (Gordon-Mills & McCandless, 1975), that would be present in the `heavily fructified' thalli extracted by Bremond et al (1987) .…”

Section: Discussionmentioning

confidence: 97%

Influence of tissue source and growth rates on dry weight and carrageenan composition of Chondrus crispus (Gigartinales, Rhodophyta)

Rivera-Carro¹,

Shacklock²

1990

Hydrobiologia

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Carrageenan analyses were conducted on vegetative female clones of Chondrus crispus that were cultured to provide tissues with differing growth rates . Tissue dry weights increased from apex to base of fronds . Total carrageenan contents were lower in apical 1 to 2 cm segments than elsewhere in the frond, except when the alga was grown at high photon irradiances . Clone 373A contained more carrageenan than clone G8. The proportion of 0 .3 M KCl-soluble polymers in the total native carrageenans varied from 44 to 92%, being highest in older tissues of fronds cultured at high photon irradiances . The apical 1 cm segments contained less KCI-soluble carrageenans than other tissues from the corresponding fronds . The KCl-soluble carrageenans, when alkali-modified and refractionated, afforded the expected kappa-iota carrageenan in > 79% yields . The remainder consisted of a polymer containing 23 .1 % SO 3Na and 8 .4% 3,6-anhydrogalactose . Lambda carrageenan was not detected . Variations in carrageenan distribution between the apical region and other parts of the frond may reflect the increasing influence of medullary tissue developed as the immature cells differentiate .

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“…Immunofluorescence studies revealed additional information especially regarding the site of sulfate groups within these celkwall polymers (Gordon-Mills and McCandless 1975, Hosford and McCandless 1975, Dinnino and McCandless 1978a, b in McCandless 1981. However, fluorescent labelled lectins have not yet been applied as cyto-and histochemical probes to red algae, although they were shown to be well suited for the detection and localization of specific carbohydrate residues at the cell surface of various species of other algal groups (Surek and v. Sengbusch 1981, v. Sengbusch et al 1982, v. Sengbusch and Müller 1983, Callow 1985.…”

Section: Introductionmentioning

confidence: 99%

Comparative Histochemical Studies of Reproductive and Gametophytic Tissue of Marine Red Algae by Means of Fluorescent and Light Microscopy

Diannelidis¹,

Kristen²

1988

Botm

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A comparative histochemical study of the localization of galactosyl residues and lectin binding sites in the cytoplasm, the cell walls and the mucilage has been undertaken in tissue of the red algae, Hypnea musciformis, Polysiphonia deusta and Halarachnion ligulatum using fluorescent probes. The lectins revealed a unique and specific binding pattern in all species examined. The data obtained with fluorescent probes were compared with those obtained by bright field light microscopy. Neutral and acidic polysaccharides were noted in the cell walls while the mucilaginous component of all species was a highly sulfated acidic polymer. Cellulose was judged reasonably to constitute a great part in the vegetative tissue of all three species examined.

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Carrageenans in the cell walls of Chondrus crispus Stack. (Rhodophyceae, Gigartinales). I. Localization with fluorescent antibody

Cited by 30 publications

References 0 publications

Algal Walls — Composition and Biosynthesis

Algal Walls — Composition and Biosynthesis

Influence of tissue source and growth rates on dry weight and carrageenan composition of Chondrus crispus (Gigartinales, Rhodophyta)

Comparative Histochemical Studies of Reproductive and Gametophytic Tissue of Marine Red Algae by Means of Fluorescent and Light Microscopy

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