Carrageenin-Induced Periodontitis as an Experimental Model in Rats Analyzed by Micro-Computerized Tomography

Abstract

Periodontitis is a common disease worldwide and is a cause of tooth loss. For these reasons, many investigators have examined periodontitis not only by in vivo studies but also in vitro studies. For in vivo studies, it is very important which animal is used and how to make experimental periodontitis easily and efficiently in animals. Although the process of periodontitis in monkeys and dogs is similar to that in humans, rats and mice are much more useful animals for experiments because of ease of handling and low cost. For the induction of periodontitis, bacterial inoculation and wire ligature methods were examined previously; however, these methods had temporal and technical problems. Therefore, a simple, easy and reliable experimental model for periodontitis using rats and/or mice is required. In this study, the authors attempted to establish a novel experimental model for periodontitis in the rat lower jaw using carrageenin-immersed silk ligature wire, and examined the change of alveolar bone morphology by micro-computerized tomography. As a result, carrageeninimmersed silk ligature wire induced inflammation of the periodontium with severe alveolar bone loss in all experimental rats. In addition, the degree of absorption of the alveolar bone depended on the dosage times of carrageenin treatment. These findings suggested that this experimental model of periodontitis using carrageenin in rats may be a useful experimental model for periodontal research.

Assessment of Vascular Endothelial Growth Factor and Matrix Metalloproteinase‐9 in the Periodontium of Rats Treated With Atorvastatin

“…This study uses ligature‐induced experimental periodontitis, which is a well‐established model of experimental periodontitis in rats and a simple and reliable technique for disease induction. Rat models are commonly used in experimental periodontitis studies, due to the similarities in structure and organization of the periodontal tissues to those of humans 35‐37 …”

Ropinirole inhibits inflammatory cytokine production in gingival epithelial cells and suppresses alveolar bone loss in an experimental rat model of periodontitis

“…We verified cardiac and respiratory arrest. We used a previously reported carrageenan-induced rat model of periodontitis (27). While the rats were under anesthesia, buccal and lingual gingiva were exfoliated using an explorer.…”

Studies on the Effects of Protamine-Reduced Peptide on Experimental Periodontitis Analyzed by Micro-Computerized Tomography

“…In addition, it is reported that NOS inhibition prevents alveolar bone resorption 42) . According to these findings, it seems that the application of PRPs to periodontitis with weak bone loss may achieve more effective results, although the authors applied PRPs to periodontitis with heavy alveolar bone loss 23) . For this reason, further additional experiment may be necessary in future.…”

“…The carrageenin-induced experimental periodontitis model 23) was used in this study. Briefly, the buccal gingiva of the right mandibular first molar of six male rats (control group n=3, …”

“…To confirm the effect of these novel PRPs against periodontitis with heavy bone loss, the authors applied PRPs to the periodontal pocket of experimental periodontitis in rats 23) twice a week for 3 consecutive weeks. Then, 3D images were taken after microcomputerized tomography (micro-CT) and the change in alveolar bone during the experiment was observed.…”