Carrageenophyte-attached and planktonic bacterial communities in two distinct bays of Vietnam: Eutrophication indicators and insights on ice-ice disease

Kopprio, Gérmán A.; Cuong, Le Huu; Luyen, Nguyen Dinh; Duc, Tran Mai; Ha, Tran Hong; Hương, Lê Mai; Gärdes, Astrid

doi:10.1016/j.ecolind.2020.107067

Cited by 20 publications

(24 citation statements)

References 63 publications

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“…However, the biofilm attached to the Kappaphycus thallus was correlated with increasing epi-endophyte growth. Previous studies have confirmed that biofilms can be detrimental for seaweeds due to their aggregation of pathogenic microorganisms and settlement spores of the various pests (Wahl et al 2012;Potin 2012;Tsiresy et al 2016), as well as infectious bacteria causing ice-ice syndrome (Arasamuthu and Edward 2018;Kopprio et al 2021). In this study, the biofilms, irrespective of the cultivation period, were easily removed by regular farm cleaning.…”

Section: Controlling the Pests Outbreaksupporting

confidence: 67%

“…The causative agents and the pathways of the ice-ice syndrome have been identified and reported (Ward et al 2020). Elevation of seawater temperature, high variability in salinity and the availability of seaweed pests have been shown to be key factors in increasing the susceptibility of Kappaphycus/Eucheuma to the infectious microbial pathogens, which can cause an iceice syndrome (Vairappan et al 2010;Pang et al 2015;Largo et al 2020;Kopprio et al 2021). Epiphytic macroalgae are also considered to be a major pest by eucheumatoid seaweed farmers, as they are associated with a high mortality of the whole or partial thallus, reduced growth rates and decreased reproduction (Vairappan 2006;Vairappan et al 2010;Wahl et al 2012;Ingle et al 2018).…”

Section: Introductionmentioning

confidence: 99%

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Seaweed aquaculture: a preliminary assessment of biosecurity measures for controlling the ice-ice syndrome and pest outbreaks of a Kappaphycus farm

et al. 2021

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The application of biosecurity in seaweed aquaculture plays an important role in reducing the impact of disease and pest outbreaks. The continuous occurrence of seaweed pests including the macroalgal epiphytes, epi-endophytic filamentous algae and biofilms on Kappaphycus farms may also potentially induce further incidences of the ice-ice syndrome. In this study, on-farm biosecurity management measures were tested on the commercially grown seaweeds Kappaphycus malesianus and Kappaphycus alvarezii during peak ice-ice season at Gallam-Gallam Village, Sabah, Malaysia. The investigation was focused on preventative control measures including the early detection of the ice-ice syndrome and pests through propagule health checks, regular cleaning of the crop thallus and associated long-line ropes and monitoring of the environment. Farm procedures and practices were also assessed in terms of their biosecurity ‘risk’ using the hazard analysis and critical control point (HCCAP) approach. Observations were replicated in two different farm management systems; one system adopted routine biosecurity measures and the other had no biosecurity measures. The results showed that the ice-ice syndrome and pest outbreak was significantly decreased by 60–75% for K. malesianus and 29–71% for K. alvarezii at the farm which adopted the routine biosecurity measures compared with the no biosecurity treatment. The biosecurity measures also significantly improved growth rate and seaweed quality. The infection levels of the epi-endophyte Melanothamnus sp. contributed to the ice-ice syndrome in K. malesianus, whilst the epiphyte coverage was correlated to the ice-ice incidence in K. alvarezii. This study provides the first evidence of biosecurity management measures significantly decreasing the incidence of the ice-ice syndrome and pests on a commercial seaweed farm.

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Section: Controlling the Pests Outbreaksupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Seaweed aquaculture: a preliminary assessment of biosecurity measures for controlling the ice-ice syndrome and pest outbreaks of a Kappaphycus farm

et al. 2021

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“…NS4 marine group is another indicator of increased phytoplankton concentration and algal blooms aforementioned [61]. The intensive aquaculture in coastal marine cages caused the dominance of NS4 marine group in Vietnam due to its strong correlations with inorganic nutrients such as ammonium and phosphate (good predictor of algae proliferation) [62]. The Arcobacter genus on the other hand, similarly to Coraliomargarita, is a common bacterial group found in coral reef fish larvae bodies [59].…”

Section: Discussionmentioning

confidence: 99%

Digital Droplet PCR-Based Environmental DNA Tool for Monitoring Cryptocaryon irritans in a Marine Fish Farm from Hong Kong

et al. 2021

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The adoption of new investigative strategies based on environmental DNA (eDNA) can be used to monitor parasites, associated bacterial microbiomes, and physical-chemical parameters in fish farms. In this study, we used the economically important and globally distributed fish ciliate parasite Cryptocaryon irritans as a model to understand the parasite abundance and potential drivers of its presence in marine fish farms. Environmental (rainfall) and physical-chemical (temperature, oxygen, salinity, pH) data collected from a marine fish farm in Hong Kong were analyzed together with the eDNA approach targeting C. irritans abundance based on digital droplet PCR and 16S metagenomics to determine associations and triggers between parasites and specific bacterial groups. Rainfall and temperature demonstrated positive associations with high abundance of C. irritans (eDNA) at the studied marine fish cage farm. However, rainfall was the only parameter tested that demonstrated a significant association with parasite eDNA, indicating that the raining season is a risky period for fish farmers in Hong Kong. Coraliomargarita was the bacterial genus with the most significant relationship with low abundance of C. irritans in water. Understanding the environmental triggers of ciliate parasites propagation and associated bacterial microbiome could elucidate new insights into environmental control, microbial management, and promote the reduction of chemical use in marine fish farms.

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“…A new thiopeptide antibiotic as microflora modulator was detected on Cutibacterium sp. (Claesen et al ., 2020) and some species of this genus might produce antibiotics on Kappaphycus striatus (Kopprio et al ., 2021). A similar role on algal defense is suspected in the other Actinobacteria Micrococcus spp.…”

Section: Discussionmentioning

confidence: 99%

“…Although recent advances in the study of the macroalgal microbiome using next generation techniques, the information is still limited and mostly derived from culture methods with their intrinsic limitations. The 16S rRNA gene approach offers broader insights on the natural composition of bacterial communities attached to algae and plays an important role in understanding algal diseases (Egan et al ., 2014; Kopprio et al ., 2021). Aims of this work were: 1) to characterize the bacterial communities of C. lentillifera in Vietnamese aquaculture, and 2) to assess the effect of an unknown disease on its bacterial community composition.…”

Section: Introductionmentioning

confidence: 99%

Bacterial community composition of the sea grape Caulerpa lentillifera: a comparison between healthy and diseased states

Kopprio

Luyen

Cuong

et al. 2021

Preprint

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The bacterial communities of the sea grape Caulerpa lentillifera were studied during a disease outbreak in Vietnam. The Rhodobacteraceae and Rhodovulum dominated the composition of healthy C. lentillifera. Clear differences between healthy and diseased cases were observed at order, genus and Operational Taxonomic Unit (OTU) level. Bacterial diversity was lower in healthy C. lentillifera, probably because of antimicrobial compounds from the macroalgae and/or from Clostridium, Cutibacterium or Micrococcus bacteria. The likely beneficial role of Bradyrhizobium, Paracoccus and Brevundimonas strains on nutrient cycling and phytohormone production was discussed. The white coloration of diseased C. lentillifera may not only be associated with pathogens but also with an oxidative response. Aquibacter, Winogradskyella and other OTUs of the family Flavobacteriaceae were hypothesized as detrimental bacteria, this family comprises some well-known seaweed pathogens. Moreover, Thalassobius OTU 2935 and 1635 may represent detrimental Rhodobacteraceae. Phycisphaera together with other Planctomycetes and Woeseia were probably saprophytes of C. lentillifera. This study offers pioneering insights on the co-occurrence of C. lentillifera-attached bacteria, potential detrimental or beneficial microbes, and a baseline for understanding the C. lentillifera holobiont. Further metagenomic and biotechnological approaches are needed to confirm functions of some microbes on this macroalgae to enhance food security in the tropics.

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Carrageenophyte-attached and planktonic bacterial communities in two distinct bays of Vietnam: Eutrophication indicators and insights on ice-ice disease

Cited by 20 publications

References 63 publications

Seaweed aquaculture: a preliminary assessment of biosecurity measures for controlling the ice-ice syndrome and pest outbreaks of a Kappaphycus farm

Seaweed aquaculture: a preliminary assessment of biosecurity measures for controlling the ice-ice syndrome and pest outbreaks of a Kappaphycus farm

Digital Droplet PCR-Based Environmental DNA Tool for Monitoring Cryptocaryon irritans in a Marine Fish Farm from Hong Kong

Bacterial community composition of the sea grape Caulerpa lentillifera: a comparison between healthy and diseased states

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